Results 81 to 90 of about 266,976 (317)

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Kank Is an EB1 Interacting Protein that Localises to Muscle-Tendon Attachment Sites in Drosophila [PDF]

, 2014
Little is known about how microtubules are regulated in different cell types during development. EB1 plays a central role in the regulation of microtubule plus ends.
Hiroyuki Ohkura (90476), Sara M R Clohisey, Clohisey Sara M. R., Ohkura, Hiroyuki, Nikola S. Dzhindzhev (207024), Hiroyuki Ohkura, Dzhindzhev Nikola S., Clohisey, Sara M R, Nikola S Dzhindzhev, Dzhindzhev, Nikola S, Ohkura Hiroyuki, Sara M. R. Clohisey (627652)   +11 more
core   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Cryo-EM structure of VASH1-SVBP bound to microtubules

eLife, 2020
The dynamic tyrosination-detyrosination cycle of α-tubulin regulates microtubule functions. Perturbation of this cycle impairs mitosis, neural physiology, and cardiomyocyte contraction.
Faxiang Li, Yang Li, Xuecheng Ye, Haishan Gao, Zhubing Shi, Xuelian Luo, Luke M Rice, Hongtao Yu   +7 more
doaj   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source

Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends

eLife, 2020
Intracellular transport relies on multiple kinesins, but it is poorly understood which kinesins are present on particular cargos, what their contributions are and whether they act simultaneously on the same cargo.
Andrea Serra-Marques, Maud Martin, Eugene A Katrukha, Ilya Grigoriev, Cathelijn AE Peeters, Qingyang Liu, Peter Jan Hooikaas, Yao Yao, Veronika Solianova, Ihor Smal, Lotte B Pedersen, Erik Meijering, Lukas C Kapitein, Anna Akhmanova   +13 more
doaj   +1 more source

Ectopic A-lattice seams destabilize microtubules [PDF]

, 2014
Natural microtubules typically include one A-lattice seam within an otherwise helically symmetric B-lattice tube. It is currently unclear how A-lattice seams influence microtubule dynamic instability.
Cross, R. A., Katsuki, Miho, Drummond, Douglas R.   +2 more
core   +1 more source

Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth

, 2021
International audienceMitotic spindle function depends on the precise regulation of microtubule dynamics and microtubule sliding. Throughout mitosis, both processes have to be orchestrated to establish and maintain spindle stability.
Gélin, Matthieu, Tran, Phong, Krüger, Lara Katharina, Houdusse, Anne, Liang Ji, Phong T Tran, Lara Katharina Krüger, Blanchoin, Laurent, Anne Houdusse, Manuel Théry, Matthieu Gélin, Théry, Manuel, Ji, Liang, Carlos Kikuti, Laurent Blanchoin, Kikuti, Carlos   +15 more
core   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Dynamic Dissection of Dynein and Kinesin-1 Cooperatively Mediated Intercellular Transport of Porcine Epidemic Diarrhea Coronavirus along Microtubule Using Single Virus Tracking

Virulence, 2021
It is now clear that the intercellular transport on microtubules by dynein and kinesin-1 motors has an important role in the replication and spread of many viruses.
Wei Hou, Wenjie Kang, Yangyang Li, Yanke Shan, Shouyu Wang, Fei Liu   +5 more
doaj   +1 more source