Results 91 to 100 of about 335,784 (179)
γ-TuRC asymmetry induces local protofilament mismatch at the RanGTP-stimulated microtubule minus end
The EMBO JournalThe γ-tubulin ring complex (γ-TuRC) is a structural template for de novo microtubule assembly from α/β-tubulin units. The isolated vertebrate γ-TuRC assumes an asymmetric, open structure deviating from microtubule geometry, suggesting that γ-TuRC closure
Bram JA Vermeulen +10 more
doaj +1 more source
Myomegalin is necessary for the formation of centrosomal and Golgi-derived microtubules
Biology Open, 2012 Summary The generation of cellular microtubules is initiated at specific sites such as the centrosome and the Golgi apparatus that contain nucleation complexes rich in γ-tubulin.
Régine Roubin +6 more
doaj +1 more source
Nucleation and the kinetics of microtubule assembly. [PDF]
Proceedings of the National Academy of Sciences, 1980 A kinetic model was developed for the purpose of interpreting scanning calorimetric data of microtubule assembly. The model consists of two steps. The first step is a highly exothermic nucleation with a strong dependence upon temperature and concentration.
M B, Jackson, S A, Berkowitz
openaire +2 more sources
The Journal of Physiology, EarlyView.Abstract figure legend Schematic outlining the activation of hypoxia‐sensitive pathways, the influence of hypoxia and associated pathways on the cytoskeleton, and the impact of these on disease progression. Abstract A highly‐regulated and dynamic cytoskeleton is vital for functional cellular physiology and the maintenance of homeostasis.
Darragh Flood, Cormac T. Taylor
wiley +1 more source
Molecular insight into microtubule nucleation by the XMAP215/γ-TuRC module
Nature CommunicationsIt has become increasingly evident in recent years that nucleation of microtubules from a diverse set of microtubule organizing centers (MTOCs) requires both the γ-tubulin ring complex (γ-TuRC) and the microtubule polymerase XMAP215.
Collin T. McManus +4 more
doaj +1 more source
Microtubules are organized independently of the centrosome in
Neural Development, 2011 Background The best-studied arrangement of microtubules is that organized by the centrosome, a cloud of microtubule nucleating and anchoring proteins is clustered around centrioles.
Nguyen Michelle M +2 more
doaj +1 more source
Nature Cell Biology, 2018 How microtubules (MTs) are generated in the cell is a major question in understanding how the cytoskeleton is assembled. For several decades, γ-tubulin has been accepted as the universal MT nucleator of the cell. Although there is evidence that γ-tubulin
Akanksha Thawani +2 more
semanticscholar +1 more source
Unveiling the Functional Role of KIF21B in Microglia Inflammatory Response
Glia, Volume 74, Issue 9, September 2026.Microglia express KIF21B in both sexes. KIF21B binds to microglia cytoskeleton; inflammation alters such interaction promoting a phagocytic state in females. In male microglia KIF21B modulates migration. ABSTRACT Microglia are brain immune cells that maintain homeostasis and respond to injury, changing cell morphology to drive inflammation, migration ...
Danny Ganchala +6 more
wiley +1 more source
eLife, 2019 Determining how microtubules (MTs) are nucleated is essential for understanding how the cytoskeleton assembles. While the MT nucleator, γ-tubulin ring complex (γ-TuRC) has been identified, precisely how γ-TuRC nucleates a MT remains poorly understood ...
Akanksha Thawani +6 more
semanticscholar +1 more source
Deep Mutational Scanning for the Study and Engineering of Protein Assemblies
Chemistry–Methods, Volume 6, Issue 8, August 2026.This review explores how deep mutational scanning (DMS) can be used to improve understanding and accelerate engineering of protein assemblies, from natural fibers to engineered nanocages. We discuss various types of protein assemblies and their characterization, provide an introduction to the DMS technique, and then highlight examples in which DMS has ...
Jenna B. Wolfanger +2 more
wiley +1 more source