Results 51 to 60 of about 5,512 (205)

Recombinase polymerase amplification assay for rapid detection of Monkeypox virus [PDF]

, 2019
In this study, a rapid method for the detection of Central and West Africa clades of Monkeypox virus (MPXV) using recombinase polymerase amplification (RPA) assay targeting the G2R gene was developed.
Abd El Wahed, Ahmed, Ademowo, Olusegun George, Böhlken-Fascher, Susanne, Czerny, Claus-Peter, Davi, Saskia Dede, Faye, Martin, Faye, Oumar, Faye, Ousmane, Hufert, Frank T, Kissenkötter, Jonas, Sall, Amadou A, Stahl-Hennig, Christiane, Weidmann, Manfred   +12 more
core   +1 more source

Comparative virology and pathology of Monkeypox virus in Syrian hamsters, C57BL/6, and BALB/c mice: A framework for preclinical model selection

Animal Models and Experimental Medicine, EarlyView.
This study compared monkeypox virus (MPXV) infection in three small animal models: Syrian hamsters, C57BL/6 mice, and BALB/c mice. Following intraperitoneal inoculation, Syrian hamsters exhibited the highest susceptibility, with rapid systemic viral dissemination, elevated splenic and renal viral burdens, and severe histopathological damage, followed ...
Xiaohui Wei, Jing Wu, Hekai Yang, Na Li, Binbin Zhao, Wanjun Peng, Na Rong, Wei Zhang, Jing Xue, Jiangning Liu   +9 more
wiley   +1 more source

Monkeypox virus (MPXV) genomics: A mutational and phylogenomic analyses of B.1 lineages

Travel Medicine and Infectious Disease, 2023
The recent increase in monkeypox (MPX) cases has attracted attention of public health authorities due to its quick spread and transmission across non-endemic regions. This outbreak, unlike previous ones, displays different epidemiological features and transmission dynamics, which appear to be largely influenced by the newly divergent MPX lineages (B.1).
Nicolas Luna, Marina Muñoz, D. Katterine Bonilla-Aldana, Luz H. Patiño, Yana Kasminskaya, Alberto Paniz-Mondolfi, Juan David Ramírez   +6 more
openaire   +2 more sources

Performance of a Novel Real-Time PCR-Based Assay for Rapid Monkeypox Virus Detection in Human Samples

Microorganisms, 2023
The ongoing epidemic of mpox, namely human monkeypox virus (MPXV) infection, requires rapid and reliable laboratory diagnosis. We report on the QIAstat-Dx viral vesicular panel PCR assay that allows the detection of (within 75 min) six vesicular disease ...
Flora Marzia Liotti, Simona Marchetti, Federico Falletta, Sara D’Onghia, Maurizio Sanguinetti, Rosaria Santangelo, Brunella Posteraro   +6 more
doaj   +1 more source

Prevalence and Molecular Profiling of Merkel Cell Polyomavirus in Patients With Monkeypox Virus Infection

Journal of Medical Virology, Volume 98, Issue 4, April 2026.
ABSTRACT Mpox, caused by Monkeypox virus (MPXV), is associated with mucosal involvement and immune modulation that may influence viral coinfections. Merkel Cell Polyomavirus (MCPyV), a ubiquitous virus capable of lifelong persistence, was investigated in 66 Mpox patients enrolled at Lazzaro Spallanzani National Institute for Infectious Diseases (Rome ...
Sara Passerini, Davide Mariotti, Sara Messina, Valentina Mazzotta, Giulia Matusali, Andrea Antinori, Valeria Pietropaolo, Fabrizio Maggi, Luigi Rosa   +8 more
wiley   +1 more source

Clinical characteristics, viral dynamics, and antibody response of monkeypox virus infections among men with and without HIV infection in Guangzhou, China

Frontiers in Cellular and Infection Microbiology
BackgroundMonkeypox virus (MPXV) is spreading globally and nearly half of the infected people were human immunodeficiency virus (HIV) positive. Therefore, an in-depth understanding of the effects of HIV infection on the outcomes of MPXV infection is ...
Huiqin Yang, Xiaoqing Xie, Mou Zeng, Yinghui Cao, Qinghong Fan, Mengling Jiang, Chunliang Lei, Jian Wang, Feng Li, Xiaoping Tang, Haisheng Yu, Linghua Li   +11 more
doaj   +1 more source

IgA Antibodies Reveal Covert Infection With Mpox Virus in People Living With HIV in the Community: A Prospective Longitudinal Cohort Survey

MedComm, Volume 7, Issue 4, April 2026.
This study identified covert infection of MPXV among people living with HIV and MPXV‐IgA was the effective indicator. The combined use of two antigen protein set of MPXV (B6R plus E8L and E8L plus H3L) yield IgA test results comparable to those obtained with the MPXV.
Rui Song, Danyang Li, Lan Chen, Xiao Wang, Qiao Zhang, Zhixia Gu, Xueqi Chi, Yuanyuan Zhang, Jing Han, Li Guo, Ronghua Jin, Lili Ren, Jianwei Wang   +12 more
wiley   +1 more source

Development and validation of a nanoplate-based digital PCR assay for absolute MPXV quantification

Journal of Virological Methods, 2023
Quantification of mpox virus (MPXV) across different human body anatomical sites can provide insights about the most likely transmission routes, so methods able to release absolute and exact quantitative values of MPXV DNA are crucial. Here, we optimized a new QIAcuity digital PCR (dPCR) protocol for the detection and quantification of MPXV DNA in ...
Specchiarello E, Carletti F, Matusali G, Abbate I, Rozera G, Minosse C, Petrivelli E, Ferraioli V, Sciamanna R, Maggi F.   +9 more
openaire   +2 more sources

Evaluation of Stability, Inactivation, and Disinfection Effectiveness of Mpox Virus

Viruses
Background: Mpox virus (MPXV) infections have increased in many countries since May 2022, increasing demand for diagnostic tests and research on the virus.
Yuwei Li, Shiyun Lv, Yan Zeng, Zhuo Chen, Fei Xia, Hao Zhang, Demiao Dan, Chunxia Hu, Yi Tang, Qiao Yang, Yaqi Ji, Jia Lu, Zejun Wang   +12 more
doaj   +1 more source

Development of a Loop-Mediated Isothermal Amplification Method for Rapid and Visual Detection of Monkeypox Virus

Microbiology Spectrum, 2022
Monkeypox virus (MPXV) is a human pathogenic virus that belongs to the genus Orthopoxvirus. In 2022, MPXV caused an unprecedented number of infections in many countries.
Junxia Feng, Guanhua Xue, Xiaohu Cui, Bing Du, Yanling Feng, Jinghua Cui, Hanqing Zhao, Lin Gan, Zheng Fan, Tongtong Fu, Ziying Xu, Shuheng Du, Yao Zhou, Rui Zhang, Hanyu Fu, Ziyan Tian, Qun Zhang, Chao Yan, Jing Yuan   +18 more
doaj   +1 more source