Results 41 to 50 of about 1,562 (148)

Structural basis for the MukB‐topoisomerase IV interaction and its functional implications in vivo [PDF]

The EMBO Journal, 2013
Chromosome partitioning in Escherichia coli is assisted by two interacting proteins, topoisomerase (topo) IV and MukB. MukB stimulates the relaxation of negative supercoils by topo IV; to understand the mechanism of their action and to define this functional interplay, we determined the crystal structure of a minimal MukB-topo IV complex to 2.3 Å ...
Seychelle M, Vos, Nichole K, Stewart, Martha G, Oakley, James M, Berger   +3 more
openaire   +2 more sources

Global dissemination of a multidrug resistant Escherichia coli clone. [PDF]

, 2014
Escherichia coli sequence type 131 (ST131) is a globally disseminated, multidrug resistant (MDR) clone responsible for a high proportion of urinary tract and bloodstream infections. The rapid emergence and successful spread of E.
A. Pascual, Alikhan, B. A. Rogers, B. M. Forde, Bruant, Canton, Castresana, Coque, Croucher, D. Gomes Moriel, D. L. Paterson, Darling, Didelot, E. Skippington, G. Dougan, Harris, Holt, J. D. D. Pitout, J. Rodriguez-Bano, Johnson, K. M. Peters, Lavigne, M. A. Schembri, M. Davies, M. Stanton-Cook, M. Totsika, M. Upton, M.-D. Phan, Martincorena, N. K. Petty, N. L. Ben Zakour, Ren, S. A. Beatson, Selkrig, T. R. Walsh, Totsika, Touchon, Uchida, Whitfield   +38 more
core   +3 more sources

Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli [PDF]

The EMBO Journal, 1999
mukF, mukE and mukB genes are essential for the process of chromosome partitioning in Escherichia coli. We have studied protein-protein interactions among MukB, MukE and MukF proteins by co-immunoprecipitation and sucrose gradient sedimentation experiments, using mukFEB null cells harboring plasmids carrying the wild-type or mutant-type mukFEB operon ...
M, Yamazoe, T, Onogi, Y, Sunako, H, Niki, K, Yamanaka, T, Ichimura, S, Hiraga   +6 more
openaire   +2 more sources

Carboxyl terminal region of the MukB protein in Escherichia coli is essential for DNA binding activity [PDF]

FEMS Microbiology Letters, 1996
The purified MukB protein of Escherichia coli has DNA binding activity and nucleotide binding activity. We have isolated a mutation, mukB1013, causing a substitution of valine at position 1379 to leucine. This mutant MukB protein was defective for DNA binding, while the ATP binding activity remained unaffected. A truncated MukB protein that is short of
A Z, Saleh, K, Yamanaka, H, Niki, T, Ogura, M, Yamazoe, S, Hiraga   +5 more
openaire   +3 more sources

Escherichia coli CrfC Protein, a Nucleoid Partition Factor, Localizes to Nucleoid Poles via the Activities of Specific Nucleoid-Associated Proteins

Frontiers in Microbiology, 2019
The Escherichia coli CrfC protein is an important regulator of nucleoid positioning and equipartition. Previously we revealed that CrfC homo-oligomers bind the clamp, a DNA-binding subunit of the DNA polymerase III holoenzyme, promoting colocalization of
Saki Taniguchi, Kazutoshi Kasho, Shogo Ozaki, Tsutomu Katayama   +3 more
doaj   +1 more source

Biochemical characterization of the Structural Maintenance of Chromosomes (SMC) complex from Bacillus subtilis [PDF]

, 2004
Structural Maintenance of Chromosomes (SMC) proteins play a key role in the chromosome dynamics throughout the cell cycle in almost all species from bacteria to eukaryotes.
Graumann, Peter (PD Dr.), Volkov, Arsen
core   +1 more source

The Localization and Action of Topoisomerase IV in Escherichia coli Chromosome Segregation Is Coordinated by the SMC Complex, MukBEF

Cell Reports, 2015
The type II topoisomerase TopoIV, which has an essential role in Escherichia coli chromosome decatenation, interacts with MukBEF, an SMC (structural maintenance of chromosomes) complex that acts in chromosome segregation.
Pawel Zawadzki, Mathew Stracy, Katarzyna Ginda, Katarzyna Zawadzka, Christian Lesterlin, Achillefs N. Kapanidis, David J. Sherratt   +6 more
doaj   +1 more source

Are SMC complexes loop extruding factors? Linking theory with fact [PDF]

, 2019
The extreme length of chromosomal DNA requires organizing mechanisms to both promote functional genetic interactions and ensure faithful chromosome segregation when cells divide.
Akai, Alipour, Alt, Anderson, Badrinarayanan, Beckouët, Brackley, Brackley, Busslinger, Bürmann, Davidson, Diebold-Durand, Dixon, Eeftens, Fennell-Fezzie, Fudenberg, Ganji, Gerlich, Gibcus, Gligoris, Goloborodko, Goloborodko, Gruber, Guacci, Haarhuis, Hadjur, Haering, Hirano, Hirano, Hopfner, Hornig, Houlard, Hu, Kamada, Kanke, Kimura, Kimura, Kimura, Kinoshita, Kojic, Kschonsak, Lavoie, Lengronne, Li, Lipp, Marko, Minnen, Mizuguchi, Murayama, Murayama, Murayama, Nasmyth, Nasmyth, Naumova, Nora, Ono, Ono, Parelho, Paulson, Petela, Piazza, Racko, Rao, Rao, Robellet, Rojowska, Rolef Ben-Shahar, Rowland, Saitoh, Saka, Sanborn, Schalbetter, Schleiffer, Schwarzer, Sexton, Shintomi, Shintomi, Srinivasan, St-Pierre, Stigler, Strunnikov, Sullivan, Sutani, Tada, Terakawa, Uhlmann, Uhlmann, Uhlmann, Unal, Vian, Walther, Wang, Wang, Wang, Wells, Wendt, Wutz, Yamamoto, Yoshimura, Zabrady, Zawadzka, Çamdere, Çamdere   +102 more
core   +1 more source

RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli [PDF]

Journal of Bacteriology, 1996
We have isolated suppressor mutants that suppress temperature-sensitive colony formation and anucleate cell production of a mukB mutation. A linkage group (smbB) of the suppressor mutations is located in the rne/ams/hmp gene encoding the processing endoribonuclease RNase E. All of the rne (smbB) mutants code for truncated RNase E polypeptides lacking a
M, Kido, K, Yamanaka, T, Mitani, H, Niki, T, Ogura, S, Hiraga   +5 more
openaire   +2 more sources

Two mutant alleles ofmukB, a gene essential for chromosome partition inEscherichia coli [PDF]

FEMS Microbiology Letters, 1994
The MukB protein is essential for chromosome partitioning in Escherichia coli and consists of 1484 amino acid residues (170 kDa). We have determined the base changes at the mutated sites of the mukB106 mutant and a newly isolated mutant, mukB33. These mutant mukB genes were each found to carry a single base-pair transition which leads to an amino acid ...
K, Yamanaka, T, Mitani, J, Feng, T, Ogura, H, Niki, S, Hiraga   +5 more
openaire   +3 more sources