Results 1 to 10 of about 465,638 (353)

Detection of alpha-thalassemias by multiplex polymerase chain reaction [PDF]

Clinical Chemistry, 1994
Abstract Although alpha-thalassemia is the most common genetic abnormality in the world, there is currently no routine laboratory method to definitively identify individuals who are affected. We describe a rapid and simple method that utilizes deletion-sensitive primers to amplify normal DNA sequences.
Lemuel J. Bowie, Poluru L. Reddy, M. Nagabhushan, P Sevigny   +3 more
openalex   +3 more sources

Multiplex polymerase chain reaction (M-PCR) for bacterial vaginosis detection

Vietnam Journal of Science, Technology and Engineering, 2022
Bacterial vaginosis (BV) is one of the most common vaginal infections in women of reproductive age. If not treated promptly, the disease can lead to serious complications affecting the fertility and long-term health of women.
Tien Sang Trieu, Van Khoa Tran, Ha My Nguyen, Thi Thu Ha Nguyen, Quang Tung Le, Minh Ngoc Phung, Minh Quang Diep, Huong Ly Vu   +7 more
doaj   +3 more sources

Diagnostic Accuracy of Multiplex Polymerase Chain Reaction in Early Onset Neonatal Sepsis [PDF]

Children, 2023
Early onset neonatal sepsis is a significant contributor to neonatal morbidity and mortality. Although blood cultures remain the diagnostic gold standard, they detect pathogens in only a minority of suspected cases.
Anja Stein, Daniel Soukup, Peter-Michael Rath, Ursula Felderhoff-Müser   +3 more
doaj   +2 more sources

Impact of multiplex polymerase chain reaction testing in patients with bacteremia [PDF]

Microbiology Spectrum
Rapid identification of pathogens in bacteremia is critical for optimizing antimicrobial therapy. The FilmArray blood culture identification 2 (BCID2) panel enables multiplex PCR-based detection of pathogens and resistance genes.
Daisuke Kitagawa, Taito Kitano, Takehito Kasamatsu, Naoyuki Shiraishi, Mai Yasuda, Mai Okada, Soma Suzuki, Madoka Sekine, Ryo Yamanishi, Ayu Mukai, Ritsuki Uejima, Yuki Suzuki, Akiyo Nakano, Ryuichi Nakano, Hisakazu Yano, Fumihiko Nakamura, Koichi Maeda   +16 more
doaj   +2 more sources

Multiplex polymerase chain reaction: A practical approach [PDF]

Journal of Clinical Laboratory Analysis, 2002
AbstractConsiderable time and effort can be saved by simultaneously amplifying multiple sequences in a single reaction, a process referred to as multiplex polymerase chain reaction (PCR). Multiplex PCR requires that primers lead to amplification of unique regions of DNA, both in individual pairs and in combinations of many primers, under a single set ...
P, Markoulatos, N, Siafakas, M, Moncany
openaire   +3 more sources

Brazilian ground beef authentication by multiplex polymerase chain reaction [PDF]

Ciência Rural, 2018
: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared
Andrey Carlos do Sacramento de Oliveira, Silvia Cristina da Silva Pedroso, Diogo José Cardilli, Fábio Pereira Leivas Leite, Gabrielle Virgínia Lopes Ferreira, Andréia Silva da Silva, Talita Bandeira Roos, Carina Martins de Moraes, Roberta Sales Sousa, Roseli do Socorro Dias Monteiro   +9 more
doaj   +3 more sources

Usefulness of Stool Multiplex Polymerase Chain Reaction Assays in Patients with Acute Diarrhea

The Korean Journal of Gastroenterology, 2022
Background/Aims: There is a recent increase in the use of stool multiplex PCR assay-based diagnostic tests in patients with acute diarrhea. We used multiplex PCR assays to analyze the distribution of diarrhea-causing bacteria and viruses, as well as the ...
Seo Hyun Kim, You Sun Kim, Seung Hyuk Kim, Won Eui Yoon, Hee Jun Myung, Jeong Seop Moon, Dong Hee Whang   +6 more
doaj   +3 more sources

MULTIPLEX POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF NEISSERIA GONORRHOEAE AND THE QUINOLONE RESISTANCE GENE IN PAKISTAN

Pakistan Armed Forces Medical Journal, 2021
Objective: To detect Neisseria gonorrhoeae from the urine of male patients reporting with active urethral discharge using multiplex polymerase chain reaction (PCR).
Hafsa Waseem, Afnan Naeem, Sakhawat Ali, Sharjeel Sarfraz, Javaid Usman, Mehreen Gilani   +5 more
doaj   +1 more source

Thermally multiplexed polymerase chain reaction [PDF]

Biomicrofluidics, 2015
Amplification of multiple unique genetic targets using the polymerase chain reaction (PCR) is commonly required in molecular biology laboratories. Such reactions are typically performed either serially or by multiplex PCR. Serial reactions are time consuming, and multiplex PCR, while powerful and widely used, can be prone to amplification bias, PCR ...
Christopher R. Phaneuf, Nikita Pak, D. Curtis Saunders, Gregory L. Holst, Joav Birjiniuk, Nikita Nagpal, Stephen Culpepper, Emily Popler, Andi L. Shane, Robert Jerris, Craig R. Forest   +10 more
openaire   +2 more sources

A simple, rapid and economic method for detecting multidrug-resistant tuberculosis

Brazilian Journal of Infectious Diseases, 2013
Objective: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. Methods: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 ...
Xia Wang, Junhua Jiao, Weihua Xu, Xiaoyan Chai, Zhenyun Li, Qingjiang Wang   +5 more
doaj   +3 more sources