Results 11 to 20 of about 465,638 (353)

Multiplex Polymerase Chain Reaction for diagnosis of gastrointestinal tuberculosis [PDF]

JGH Open, 2018
Background and AimTo evaluate the role of multiplex polymerase chain reaction (PCR) for diagnosis of gastrointestinal tuberculosis (GITB).MethodsThis was a prospective observational study conducted from July 2015 to November 2016 at a tertiary care teaching institution in north India.
Sarthak Malik, Kusum Sharma, Kim Vaiphei, Narendra Dhaka, Neha Berry, Pankaj Gupta, Megha Sharma, Bipadabhanjan Mallick, Rakesh Kochhar, Saroj K Sinha   +9 more
openaire   +3 more sources

Role of multiplex polymerase chain reaction in diagnosing tubercular meningitis

Journal of Laboratory Physicians, 2017
AbstractTuberculous meningitis (TBM) is one of the most serious manifestations of extrapulmonary tuberculosis. Timely and accurate diagnosis provides a favorable prognosis in patients with TBM. The study evaluated the use of multiplex polymerase chain reaction (PCR) in the diagnosis of TBM. A study was conducted on 74 patients clinically suspected with
Berwal, Anupam, Chawla, Kiran, Vishwanath, Shashidhar, Shenoy, Vishnu Prasad   +3 more
openaire   +3 more sources

The Detection of Foodborne Pathogenic Bacteria in Seafood Using a Multiplex Polymerase Chain Reaction System. [PDF]

Foods, 2022
Multiplex polymerase chain reaction (PCR) assays are mainly used to simultaneously detect or identify multiple pathogenic microorganisms. To achieve high specificity for detecting foodborne pathogenic bacteria, specific primers need to be designed for ...
Li P, Feng X, Chen B, Wang X, Liang Z, Wang L.   +5 more
europepmc   +2 more sources

Analysis of a Novel Multiplex Polymerase Chain Reaction Assay as a Sensitive Tool for the Diagnosis of Indeterminate and Tuberculoid Forms of Leprosy

International Journal of Mycobacteriology, 2017
Objective/Background: Clinical diagnosis of indeterminate and tuberculoid leprosy is often difficult due to limited and confounding signs and symptoms. In the current study, we evaluated the utility of new multiplex polymerase chain reaction (PCR) using ...
V.S. Chaitanya, Luz Cuello, Madhusmita Das, A Sudharsan, Pavithrra Ganesan, K. Kanmani, Lakshmi Rajan, Mannam Ebenezer   +7 more
openalex   +2 more sources

Detection and characterization of meat adulteration in various types of meat products by using a high-efficiency multiplex polymerase chain reaction technique

Frontiers in Nutrition, 2022
Identification of meat authenticity is a matter of increasing concerns due to religious, economical, legal, and public health reasons. However, little is known about the inspection of eight meat species in one tube reaction due to technological challenge
Caijiao Yang, Guowei Zhong, Song Zhou, Yi-tong Guo, D. Pan, Sha Wang, Qianqian Liu, Q. Xia, Zhendong Cai   +8 more
semanticscholar   +1 more source

Examining the clinical impact of rapid multiplex polymerase chain reaction‐based diagnostic testing for bloodstream infections in a national cohort of the Veterans Health Administration

Pharmacotherapy, 2022
Bloodstream infections (BSIs) are a significant cause of mortality. Use of a rapid multiplex polymerase chain reaction‐based blood culture identification panel (BCID) may improve antimicrobial utilization and clinical outcomes by shortening the time to ...
Nicholas S. Britt, K. Khader, Tao He, Tina M. Willson, Atim Effiong, T. Timbrook, Emily M. Potter, T. Lodise   +7 more
semanticscholar   +1 more source

Review of the role of gastrointestinal multiplex polymerase chain reaction in the management of diarrheal illness

Journal of Gastroenterology and Hepatology, 2021
Acute and chronic diarrheal illness secondary to gastrointestinal infection is a significant cause of morbidity and mortality around the world. A cornerstone of management includes prompt diagnosis and appropriate treatment of culprit pathogens.
Readon Teh, Tee De, E. Tan, K. Fan, Calvin J. Koh, P. Tambyah, J. Oon, N. Tee, K. Siah   +8 more
semanticscholar   +1 more source

Identification of Dermatophytes Using Multiplex Polymerase Chain Reaction [PDF]

Annals of Dermatology, 2011
Multiplex polymerase chain reaction (PCR) allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other organisms simultaneously within a single test tube, and the scope of its use has been expanding widely in the field of clinical microbiology in recent years.To assess the ...
Kim, Ji Young, Choe, Yong Beom, Ahn, Kyu Joong, Lee, Yang Won   +3 more
openaire   +2 more sources

Enhanced detection and serotyping of using multiplex polymerase chain reaction [PDF]

Korean Journal of Pediatrics, 2012
PurposeMethods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient ...
Jong Gyun Ahn, Seong Yeol Choi, Dong Soo Kim, Ki Hwan Kim   +3 more
doaj   +1 more source

Case Report: Identification of Maternal Low-Level Mosaicism in the Dystrophin Gene by Droplet Digital Polymerase Chain Reaction

Frontiers in Genetics, 2021
Germline mosaicism should be suspected when the same de novo mutations are identified in a second pregnancy with asymptomatic parents. Our study aims to find a feasible approach to reveal the existence of germline mosaicism.
Pengzhen Jin, Pengzhen Jin, Xiaoyang Gao, Xiaoyang Gao, Miaomiao Wang, Miaomiao Wang, Yeqing Qian, Yeqing Qian, Jingjin Yang, Jingjin Yang, Yanmei Yang, Yanmei Yang, Yuqing Xu, Yuqing Xu, Yanfei Xu, Yanfei Xu, Minyue Dong, Minyue Dong   +17 more
doaj   +1 more source