Frontiers in Genetics, 2021 Germline mosaicism should be suspected when the same de novo mutations are identified in a second pregnancy with asymptomatic parents. Our study aims to find a feasible approach to reveal the existence of germline mosaicism.
Pengzhen Jin +17 more
doaj +1 more source
Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt [PDF]
Veterinary World, 2016 Aim: This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella ...
Hazem Mohammed Ibrahim +3 more
doaj +1 more source
Chemically modified primers for improved multiplex polymerase chain reaction [PDF]
Analytical Biochemistry, 2009 Multiplex polymerase chain reaction (PCR), the amplification of multiple targets in a single reaction, presents a new set of challenges that further complicate more traditional PCR setups. These complications include a greater probability for nonspecific amplicon formation and for imbalanced amplification of different targets, each of which can ...
Jonathan, Shum, Natasha, Paul
openaire +2 more sources
Multiplex Polymerase Chain Reaction for diagnosis of gastrointestinal tuberculosis [PDF]
JGH Open, 2018 Background and AimTo evaluate the role of multiplex polymerase chain reaction (PCR) for diagnosis of gastrointestinal tuberculosis (GITB).MethodsThis was a prospective observational study conducted from July 2015 to November 2016 at a tertiary care teaching institution in north India.
Sarthak Malik +9 more
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Interspecies comparison of probiotics isolated from different animals [PDF]
Veterinary World, 2018 Aim: The aim of the current study was to isolate and identify naturally occurring probiotic Lactobacillus species in different animals with the different environmental background including fish, and farm animals to investigate interspecies differences in
Amr M. Abdou +5 more
doaj +1 more source
Comparison of PCR fingerprinting techniques for the discrimination of Salmonella enterica subsp. enterica serovar Weltevreden isolated from indigenous vegetables in Malaysia. [PDF]
, 2008 Salmonella enterica subsp. enterica (S.) serovar Weltevreden has emerged as a public health problem in many countries. Genomic DNA of S. Weltevreden from indigenous vegetables namely 'selom' (Oenanthe stolonifera), 'pegaga' (Centella asiatica), 'kesum ...
Awang Saleh, Noorzaleha +5 more
core +1 more source
Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection. [PDF]
, 2019 BACKGROUND:Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants
A Bak +35 more
core +1 more source
A search for Clostridium difficile ribotypes 027 and 078 in Brazil
Brazilian Journal of Infectious Diseases, 2014 Toxigenic strains of Clostridium difficile may be disseminating. Here we prospectively screened patients with nosocomial diarrhoea in two hospitals in Brazil. To identify C.
Alexandre de Almeida Monteiro +4 more
doaj +1 more source
Species-specific Real Time-PCR primers/probe systems to identify fish parasites of the genera Anisakis, Pseudoterranova and Hysterothylacium (Nematoda: Ascaridoidea) [PDF]
, 2018 Ascaridoid nematodes belonging to the genera Anisakis and Pseudoterranova are heteroxenous parasites, involving marine mammals as definitive hosts in their life-cycles, whereas crustaceans (krill), fish and squids acting as intermediate/paratenic hosts.
Colantoni, Alessandra +5 more
core +1 more source
Virology Journal, 2019 Background Conventional assays to titrate polioviruses usually test serial dilutions inoculated into replicate cell cultures to determine a 50% cytopathic endpoint, a process that is both time-consuming and laborious.
Hasmik Manukyan +5 more
doaj +1 more source