Occurrence of Pectinatus and Megasphaera in the major UK breweries [PDF]
The occurrence of beer spoilage bacteria belonging to the genera Pectinatus and Megasphaera in ten major UK breweries was investigated. The sampling points were selected from fermentation areas, beer conditioning areas and beer bottling and canning sites.
Hill, Anne Elizabeth +2 more
core +1 more source
Molecular analysis of PKU-associated PAH mutations: a fast and simple genotyping test [PDF]
: Neonatal screening for phenylketonuria (PKU, OMIM: 261600) was introduced at the end of the 1960s. We developed a rapid and simple molecular test for the most frequent phenylalanine hydroxylase (PAH, Gene ID: 5053) mutations.
Antonio Angeloni +9 more
core +1 more source
Results of Multiplex Polymerase Chain Reaction Assay to Identify Urethritis Pathogens
Objective: The purpose of this study was to evaluate the results of multiplex polymerase chain reaction (PCR) test applied to identify the pathogens in male patients who attended our urology clinic with a pre-diagnosis of urethritis related with sexual ...
Mehmet Sarıer +4 more
doaj +1 more source
Introduction: The rapidly increasing extended-spectrum β-lactamase-producing Pseudomonas aeruginosa is a threat to health. This study aims to detect the rpoD gene and blaOXA-10 and blaOXA-48 genes in imipenem-resistant P.
Aghdas Bibi Hashemi +3 more
doaj +1 more source
Outcomes and clinical relevance of stool multiplex bacterial polymerase chain reaction in patients with acute diarrhea: single center experience [PDF]
Background/Aims Diagnostic stool multiplex polymerase chain reaction (PCR) testing has attracted considerable interest, because of its high sensitivity, short turnaround time, and ability to detect multiple organisms simultaneously.
Won Gun Kwack +4 more
doaj +1 more source
Human ascariasis: diagnostics update [PDF]
Soil-transmitted helminths (STHs) infect over one billion people worldwide. Ascariasis may mimic a number of conditions, and individual clinical diagnosis often requires a thorough work-up.
Jourdan, Peter M., Lamberton, Poppy H.L.
core +1 more source
Specific detection of Salmonella spp. by multiplex polymerase chain reaction [PDF]
Three sets of oligonucleotide primers were used in the polymerase chain reaction (PCR) assay to detect Salmonella species. phoP primers specific to the phoP/phoQ loci of coliform pathogenic bacteria such as Salmonella, Shigella, Escherichia coli, and Citrobacter species served as presumptive indicators of enteric bacteria.
J S, Way +5 more
openaire +2 more sources
Detection of Microsatellite Instability by Fluorescence Multiplex Polymerase Chain Reaction [PDF]
We have created a clinical molecular diagnostic assay to test for microsatellite instability (MSI) at multiple loci simultaneously in paraffin-embedded surgical pathology colon resection specimens. This fluorescent multiplex polymerase chain reaction (PCR) assay analyzes the five primary microsatellite loci recommended at the 1997 National Cancer ...
K D, Berg +5 more
openaire +2 more sources
Molecular methods for genomic analyses of variant PML-RARA or other RARA-related chromosomal translocations in acute promyelocytic leukemia [PDF]
TO THE EDITOR: We read an interesting paper by Palta et al. in a recent issue of the Korean Journal of Hematology titled, "ZBTB16-RARA variant of acute promyelocytic leukemia with tuberculosis: a case report and review of literature" [1].
Kim, Min Jin +4 more
core +1 more source
NGS-PrimerPlex: High-throughput primer design for multiplex polymerase chain reactions [PDF]
Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including developing new targeted next-generation sequencing (NGS) panels. We present NGS-PrimerPlex, an efficient and versatile command-line application that designs primers for different refined types of amplicon-based genome target ...
Andrey Kechin +5 more
openaire +4 more sources

