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Highly Efficient Site-Specific and Cassette Mutagenesis of Plasmids Harboring GC-Rich Sequences [PDF]
CellsGC-rich sequences affect DNA replication, recombination and repair, as well as RNA transcription in vivo. Such sequences may also impede site-directed mutagenesis in vitro.
Paulina Varela-Castillo +4 more
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Site-directed mutagenesis: colony growth [PDF]
BioTechniques, 2011 This month's question from the Molecular Biology Forums (online at molecularbiology. forums.biotechniques.com) comes from the "General Methods" section. Entries have been edited for concision and clarity.
Kristie Nybo
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General Method for Site-Directed Mutagenesis [PDF]
BioTechniques, 2001 Reiner Andag, Ekkehard Schütz
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BioTechniques, 2022 Despite the development of various methods and commercial kits, site-directed mutagenesis of large plasmids remains a challenge in many laboratories.
Zhibo Yang, Zan Chen, Yueping Zhang
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Site-directed mutagenesis [PDF]
Biochemical Journal, 1986 The development of genetic engineering, whereby a specific gene or cDNA (c is copy or complementary) can be isolated as part of a minichromosome that can replicate and be expressed (by transcription and translation) in a living cell, has made possible in vitro techniques for micromanipulation (i.e.
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Scientific Reports, 2021 Site-directed mutagenesis for large plasmids is a difficult task that cannot easily be solved by the conventional methods used in many laboratories. In this study, we developed an effective method for Site-directed Mutagenesis for Large Plasmids (SMLP ...
Kewei Zhang +11 more
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BioTechniques, 2005 Several primer prediction programs have been developed for a variety of applications. However, none of these tools allows the prediction of a large set of primers for whole gene site-directed mutagenesis experiments using the megaprimer method. We report
Ulrich Krauss, Thorsten Eggert
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Site-Directed Mutagenesis of Large Plasmids
BioTechniques, 1998 A protocol combining recombination PCR and long-distance PCR is demonstrated to be highly accurate and rapid for sitedirected mutagenesis of large (>10 kb) plasmids.
Susan A. Nadin-Davis, Shirley C. Chang
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REMP software to introduce a screening REstriction site in site-directed Mutagenesis Primer
SoftwareX, 2021 Insertion of silent mutations allowing for restriction site modification aids in the screening of successful mutants during site directed mutagenesis. Introducing a new restriction site requires the analysis of degenerate sequences within mutant primer ...
Madhavi Latha Yadav Bangaru, PhD +3 more
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Combining rational and random strategies in β-glucosidase Zm-p60.1 protein library construction. [PDF]
PLoS ONE, 2014 Saturation mutagenesis is a cornerstone technique in protein engineering because of its utility (in conjunction with appropriate analytical techniques) for assessing effects of varying residues at selected positions on proteins' structures and functions.
Dušan Turek +3 more
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