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Advances in semi-rational design of terpene synthases and their modifying enzymes. [PDF]
AMB ExpressLiang X +8 more
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Identification of fidelity-determined residues of Porcine reproductive and respiratory syndrome virus through structural alignment. [PDF]
VirulenceGao X +8 more
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2010
The technique of site-directed mutagenesis has been used to characterize gene and protein structure-function relationships, protein-protein interactions, binding domains of proteins, or active sites of enzymes for the last three decades. In this technique, a nucleotide sequence of interest is experimentally altered using synthetic oligonucleotides. The
Carrigan, Patricia E. +2 more
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The technique of site-directed mutagenesis has been used to characterize gene and protein structure-function relationships, protein-protein interactions, binding domains of proteins, or active sites of enzymes for the last three decades. In this technique, a nucleotide sequence of interest is experimentally altered using synthetic oligonucleotides. The
Carrigan, Patricia E. +2 more
openaire +2 more sources
2013
Site-directed mutagenesis is a PCR-based method to mutate specified nucleotides of a sequence within a plasmid vector. This technique allows one to study the relative importance of a particular amino acid for protein structure and function. Typical mutations are designed to disrupt or map protein-protein interactions, mimic or block posttranslational ...
openaire +3 more sources
Site-directed mutagenesis is a PCR-based method to mutate specified nucleotides of a sequence within a plasmid vector. This technique allows one to study the relative importance of a particular amino acid for protein structure and function. Typical mutations are designed to disrupt or map protein-protein interactions, mimic or block posttranslational ...
openaire +3 more sources