Results 41 to 50 of about 347,517 (297)

Site-Directed Mutagenesis

, 1983
In the preceding chapters dealing with modifications of nucleic acids it is assumed that, in general, these chemical reactions are randomly located along the genome. Exceptions occur when protein-nucleic interactions or nucleic acid secondary structure playa role.
B. Singer, D. Grunberger
openaire   +3 more sources

High-throughput site-directed mutagenesis using oligonucleotides synthesized on DNA chips

BioTechniques, 2005
Site-directed mutagenesis has greatly helped researchers both to understand the precise role of specific residues in coding sequences and to generate variants of proteins that have acquired new characteristics.
Didier Saboulard, Vincent Dugas, Mehdi Jaber, Jérôme Broutin, Eliane Souteyrand, Julien Sylvestre, Marc Delcourt   +6 more
doaj   +1 more source

Different Approaches For Protein Engineering In Industrial Biotechnology [PDF]

, 2011
Protein engineering is the novel field which has wide applications from pharmaceutics, industry, commercial, laundry and research. It may apply rational design or non rational design or both.
Israr Khan, Muhammad Waheed Akhtar
core   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Involvement of BcYak1 in the Regulation of Vegetative Differentiation and Adaptation to Oxidative Stress of Botrytis cinerea

Frontiers in Microbiology, 2018
Yak1, a member of the dual-specificity tyrosine phosphorylation-regulated protein kinases, plays an important role in diverse cellular processes in fungi. However, to date, the role of BcYak1 in Botrytis cinerea, the causal agent of gray mold diseases in
Qianqian Yang, Jianan Zhang, Jicheng Hu, Xue Wang, Binna Lv, Wenxing Liang   +5 more
doaj   +1 more source

An efficient one-step site-directed and site-saturation mutagenesis protocol [PDF]

, 2017
We have developed a new primer design method based on the QuickChange™ site-directed mutagenesis protocol, which significantly improves the PCR amplification efficiency.
Baumann, Ulrich, Reymond, Jean-Louis, Zheng, Lei   +2 more
core  

Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027 [PDF]

, 2016
Comparative analysis of the enediyne biosynthetic gene clusters revealed sets of conserved genes serving as outstanding candidates for the enediyne core.
Babnigg, Gyorgy, Bigelow, Lance, Bingman, Craig A., Chang, Changsoo, Chang, Chin-Yuan, Clancy, Shonda, Crnovcic, Ivana, Huang, Tingting, Joachimiak, Andrzej, Kim, Youngchang, Lohman, Jeremy R., Ma, Ming, Phillips, George N. Jr., Rudolf, Jeffrey D., Shen, Ben, Yan, Xiaohui, Yang, Dong, Yennamalli, Ragothaman M.   +17 more
core   +2 more sources

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Identification of amino acid residues in the ligand binding repeats of LDL receptor important for PCSK9 binding[S]

Journal of Lipid Research, 2019
Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes LDL receptor (LDLR) degradation, increasing plasma levels of LDL cholesterol and the risk of cardiovascular disease.
Shi-jun Deng, Adekunle Alabi, Hong-mei Gu, Ayinuer Adijiang, Shucun Qin, Da-wei Zhang   +5 more
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source