Results 61 to 70 of about 347,517 (297)

Hijacking emergency granulopoiesis: Neutrophil ontogeny and reprogramming in cancer

Molecular Oncology, EarlyView.
Neutrophils are highly plastic innate immune cells; their functions in cancer extend beyond the tumour microenvironment. This Review summarises current understanding of neutrophil maturation and heterogeneity and highlights tumour‐induced granulopoiesis as a systemic programme that expands immature, immunosuppressive neutrophils via tumour‐derived ...
Gabriela Marinescu, Yi Feng
wiley   +1 more source

Site-directed mutagenesis of the proposed catalytic amino acids of the Sindbis virus capsid protein autoprotease [PDF]

, 1990
The structural proteins of Sindbis virus are translated as a polyprotein precursor that is cleaved upon translation. The capsid protein is postulated to be a serine protease that releases itself from the N terminus of the nascent polyprotein by ...
Hahn, Chang S., Strauss, James H.
core  

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Consensus mutagenesis reveals that non-helical regions influence thermal stability of horseradish peroxidase [PDF]

, 2008
The enzyme horseradish peroxidase has many uses in biotechnology but a stabilized derivative would have even wider applicability. To enhance thermal stability, we applied consensus mutagenesis (used successfully with other proteins) to recombinant ...
Amin, Arnold, Azevedo, Barry J. Ryan, Bonnaffe, Carlsson, Ciarán Ó'Fágáin, Dunford, Duroux, Eijsink, Eremin, Fishman, Flores, French, Gajhede, Garcia, Gonera, Grigorenko, Ignatenko, Kamal, Kamal, Kuhlmeyer, Lehmann, Lehmann, Lei, Lesk, Lin, Lipovsek, Liu, Magliery, Maniatis, Mary J. O'Connell, McEldoon, Miland, Miland, Miyazaki, Morawski, Nielsen, O'Brien, O'Brien, Pina, Ryan, Ryan, Ryan, Ryan, Ryan, Smith, Steipe, Tams, Thompson, van den Burg, Veitch, Wang, Watanabe, Welinder, Yang, Yang   +56 more
core   +1 more source

Engineering tandem VHHs to target different epitopes to enhance antibody‐dependent cell‐mediated cytotoxicity

FEBS Open Bio, EarlyView.
Tandem VHH targeting distinct EGFR epitopes were engineered into a monovalent bispecific antibody (7D12‐EGA1‐Fc) with more potent ADCC without increasing affinity to EGFR. Structural modeling of 7D12‐EGA1‐Fc showed cross‐linking of separate EGFR domains to enhance CD16a engagement on NK cells.
Yuqiang Xu, Hao Jiang, Limin Chen, Fulai Zhou, Ying Jin, Mark L. Chiu   +5 more
wiley   +1 more source

A single amino acid substitution in the alpha 3 domain of an H-2 class I molecule abrogates reactivity with CTL. [PDF]

, 1987
We previously described a somatic cell expressing a variant H-2Dd molecule that did not serve as a target for alloreactive anti-Dd CTL. The mutant cell line had been isolated by its failure to express a serological epitope present on the H-2Dd alpha 3 ...
Bluestone, JA, Potter, TA, Rajan, TV
core   +2 more sources

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Selection Strategy for Site-Directed Mutagenesis Based on Altered β-Lactamase Specificity

BioTechniques, 1998
Conventional approaches to oligonucleotide-directed mutagenesis rely upon the application of a selection strategy to maximize mutagenesis efficiencies.
Christine A. Andrews, Scott A. Lesley
doaj   +1 more source

Converting CooA from a Carbon Monoxide to an Oxygen-Sensing Heme Protein Transcription Factor: Investigations into the Structure and Mechanism of Gas Binding [PDF]

, 2011
CooA is a carbon monoxide-sensing (CO-sensing) heme protein transcription factor that regulates gene activation in several bacteria and, importantly, is a convenient model for studying analogous proteins in the human body.
Sedoris, Amanda, Wagoner, Josh
core   +2 more sources

Plasmodium falciparum glyoxalase II: Theorell-Chance product inhibition patterns, rate-limiting substrate binding via Arg(257)/Lys(260), and unmasking of acid-base catalysis [PDF]

, 2009
Glyoxalase II (GloII) is a ubiquitous thioester hydrolase catalyzing the last step of the glutathione-dependent conversion of 2-oxoaldehydes to 2-hydroxycarboxylic acids.
Elia A.C., Hamilton D.S., Penninckx M.J., Ridderström M., Ridderström M., Thornalley P.J., Thornalley P.J.   +6 more
core   +1 more source