Results 191 to 200 of about 43,575 (246)

Regulation of murine myelin proteolipid protein gene expression

Journal of Neuroscience Research, 1997
To identify putative sequences that direct cell type-specific expression and/or enhance proteolipid protein (PLP) gene expression, glial or nonglial cells were transfected with various PLP-luciferase constructs that collectively span the entire mouse PLP-specific DNA present in a transgene known to direct cell type specificity in transgenic mice. These
P A, Wight, A, Dobretsova, W B, Macklin
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Selective Expression of DM‐20, an Alternatively Spliced Myelin Proteolipid Protein Gene Product, in Developing Nervous System and in Nonglial Cells

Journal of Neurochemistry, 1992
K. Ikenaka, T. Kagawa, K. Mikoshiba
semanticscholar   +3 more sources

Myelin Proteolipid Protein Contains Thioester‐Linked Fatty Acids

Journal of Neurochemistry, 1990
Abstract: Myelin proteolipid protein (PLP) is known to contain long‐chain, covalently bound fatty acids. Previous studies, including our own, have suggested the occurrence of an oxyester type of linkage between fatty acids and PLP. However, we found that protein‐SH groups are required in the acylation reaction, suggesting the possible presence of ...
O A, Bizzozero, L K, Good
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Regulation of rodent myelin proteolipid protein gene expression

Neuroscience Letters, 1992
The regulation of rodent proteolipid protein (PLP) gene expression was studied during rat development and in cultured cells. Nuclear run-on assays demonstrate a strong transcriptional component associated with the developmental regulation of the PLP mRNA.
J L, Cook, S, Irias-Donaghey, P L, Deininger   +2 more
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Deacylation of myelin proteolipid protein in organic solvents

Journal of Neuroscience Research, 1985
AbstractA procedure has been developed for the deacylation of the hydrophobic, myelin proteolipid apoprotein using hydroxylamine in an alkaline organic solvent medium. Complete removal of covalently bound fatty acids was obtained after 4 hr of treatment.
O A, Bizzozero, F, Dominguez, J M, Pasquini, E F, Soto   +3 more
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PROTEOLIPID PROTEIN: SYNTHESIS AND ASSEMBLY INTO QUAKING MOUSE MYELIN

Journal of Neurochemistry, 1979
AbstractThe incorporation of radioactive glycine into the major myelin proteolipid protein isolated from whole brain and from purified myelin of Quaking mice and normal littermates was compared. In a typical experiment, four Quaking mice and four littermate controls were injected intracranially with 250 μCi [2‐3H]glycine and 25 μCi [U‐14C]glycine ...
Greenfield, S, Williams, N I, White, M, Brostoff, S W, Hogan, E L   +4 more
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Radioimmunoassay for Central Nervous System Myelin‐Specific Proteolipid Protein

Journal of Neurochemistry, 1981
Abstract: A double‐antibody radioimmunoassay (RIA) has been developed with antisera to purified rat brain myelin proteolipid protein (PLP). The addition of Triton X‐100 allowed antibody‐antigen interaction and immune precipitation in the presence of sodium dodecyl sulfate (SDS).
J L, Trotter, L, Lieberman, F L, Margolis, H C, Agrawal   +3 more
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Pressure-induced dissociation of aggregates of myelin proteolipid protein

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
Sedimentation velocity and equilibrium experiments have revealed an extremely pressure-sensitive aggregation of myelin proteolipid protein in the presence of Triton X-100, dissociation of the protein aggregate being observed at pressures that are several orders of magnitude lower than those effecting disaggregation of many other proteins. These results
Gow A., Winzor D.J., Smith R.
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Immunolocalization of proteolipid protein peptide 103–116 in myelin

Journal of Neuroscience Research, 1994
AbstractDetermination of the topographic orientation of proteolipid protein (PLP) within myelin is part of an overall understanding of the functions of PLP and the roles of its multiple domains in diseases that primarily affect central nervous system (CNS) myelin.
Sobel, RA, Greer, JM, Isaac, J, Fondren, G, Lees, MB   +4 more
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Proteolipid protein interactions in transfectants: Implications for myelin assembly

Journal of Neuroscience Research, 1994
AbstractThe proteolipid proteins (PLP and DM20) are major constituents of CNS myelin, but how they are delivered to and organized within the oligodendrocyte plasma membrane is incompletely understood. We have expressed both PLP and DM20 singly or together in a host cell line, HeLa.
M. P. Sinoway, K. Kitagawa, S. Timsit, G. A. Hashim, G. A. Hashim, D. R. Colman   +5 more
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