Results 21 to 30 of about 5,726 (179)
Absence of miRNA-146a Differentially Alters Microglia Function and Proteome [PDF]
Frontiers in Immunology, 2020 Background: MiR-146a is an important regulator of innate inflammatory responses and is also implicated in cell death and survival.Methods: By sorting CNS resident cells, microglia were the main cellular source of miR-146a.
Nellie A. Martin +20 more
doaj +2 more sources
Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton
, 2012 Myelin, the multilayered membrane that enwraps and insulates neuronal axons for fast signal propagation, is a plasma membrane specialization of oligodendrocytes and Schwann cells in the central and peripheral nervous system, respectively. Here we provide our lab protocols for the puri fi cation of myelin from mouse brains and for gel-based and gel-free
Jahn, O. +4 more
openaire +3 more sources
Alzheimer disease pathology and the cerebrospinal fluid proteome
Alzheimer’s Research & Therapy, 2018 Background Altered proteome profiles have been reported in both postmortem brain tissues and body fluids of subjects with Alzheimer disease (AD), but their broad relationships with AD pathology, amyloid pathology, and tau-related neurodegeneration have ...
Loïc Dayon +11 more
doaj +2 more sources
Mitochondria in Myelinating Oligodendrocytes: Slow and Out of Breath?
Metabolites, 2021 Myelin is a lipid-rich membrane that wraps around axons and facilitates rapid action potential propagation. In the brain, myelin is synthesized and maintained by oligodendrocytes.
Niklas Meyer, Johanne Egge Rinholm
doaj +1 more source
The proteome of remyelination is different from that of developmental myelination [PDF]
, 2021 Abstract Loss of myelin underlies the pathology of several neurological disorders of diverse etiology. CNS remyelination by adult oligodendrocyte progenitor cells (OPCs) can occur but it differs from developmental myelination carried out by neonatal OPCs. We asked whether the myelin proteome of remyelinated regions is
Joana Paes de Faria +13 more
openaire +1 more source
Study on Tissue Homogenization Buffer Composition for Brain Mass Spectrometry-Based Proteomics
Biomedicines, 2022 Mass spectrometry-based proteomics aims to study the proteome both qualitatively and quantitatively. A key step in proteomic analysis is sample preparation, which is crucial for reliable results.
Adam Aleksander Karpiński +7 more
doaj +1 more source
Biology, 2022 Proteomic analysis revealed the preservation of many proteins in the Heslington brain (which is at least 2600-year-old brain tissue uncovered within the skull excavated in 2008 from a pit in Heslington, Yorkshire, England).
Aaron S. Mohammed, Vladimir N. Uversky
doaj +1 more source
Neural Plasticity, 2023 Several microRNAs (miRNAs), including miR-23 and miR-27a have been reportedly involved in regulating myelination in the central nervous system. Although miR-23 and miR-27a form clusters in vivo and the clustered miRNAs are known to perform complementary ...
Yuji Tsuchikawa +8 more
doaj +1 more source
Proteomic mapping provides powerful insights into functional myelin biology [PDF]
Proceedings of the National Academy of Sciences, 2004 Myelin is a dynamic, functionally active membrane necessary for rapid action potential conduction, axon survival, and cytoarchitecture. The number of debilitating neurological disorders that occur when myelin is disrupted emphasizes its importance. Using high-resolution 2D gel electrophoresis, mass spectrometry, and immunoblotting, we have developed an
Christopher M, Taylor +6 more
openaire +2 more sources
Identification of Myelin Basic Protein Proximity Interactome Using TurboID Labeling Proteomics
Cells, 2023 Myelin basic protein (MBP) is one of the key structural elements of the myelin sheath and has autoantigenic properties in multiple sclerosis (MS). Its intracellular interaction network is still partially deconvoluted due to the unfolded structure, abnormally basic charge, and specific cellular localization.
Evgeniya V. Smirnova +6 more
openaire +3 more sources