Results 1 to 10 of about 19,716 (205)

Development of an Anti-Zearalenone Nanobody Phage Display Library and Preparation of Specific Nanobodies [PDF]

Current Issues in Molecular Biology
Zearalenone (ZEN), a toxic estrogenic mycotoxin in cereals, threatens human and animal health through reproductive, immune, and cytotoxic effects, necessitating sensitive detection methods.
Ying Zeng, Yiying Hu, Ganying Chen, Qingqing Feng, Ruiting Wang, Zhilin Zhang, Jinxian Chen, Junbin Liao, Danrong Lin, Wei Zhu   +9 more
doaj   +2 more sources

Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

Microbial Cell Factories, 2023
Background Ablynx NV, a subsidiary of Sanofi, has a long-standing focus on the development of Nanobody® molecules as biopharmaceuticals (Nanobody® is a registered trademark of Ablynx NV). Nanobody molecules are single variable domains, and they have been
Manu De Groeve, Bram Laukens, Peter Schotte   +2 more
doaj   +2 more sources

NanoBERTa-ASP: predicting nanobody paratope based on a pretrained RoBERTa model

BMC Bioinformatics
Background Nanobodies, also known as VHH or single-domain antibodies, are unique antibody fragments derived solely from heavy chains. They offer advantages of small molecules and conventional antibodies, making them promising therapeutics.
Shangru Li, Xiangpeng Meng, Rui Li, Bingding Huang, Xin Wang   +4 more
doaj   +2 more sources

Innovative CDR grafting and computational methods for PD-1 specific nanobody design [PDF]

Frontiers in Bioinformatics
IntroductionThe development of nanobodies targeting Programmed Cell Death Protein-1 (PD-1) offers a promising approach in cancer immunotherapy. This study aims to design and characterize a PD-1-specific nanobody using an integrated computational and ...
Jagadeeswara Reddy Devasani, Girijasankar Guntuku, Nalini Panatula, Murali Krishna Kumar Muthyala, Mary Sulakshana Palla, Teruna J. Siahaan   +5 more
doaj   +2 more sources

Exploiting Nanobodies in the Detection and Quantification of Human Growth Hormone via Phage-Sandwich Enzyme-Linked Immunosorbent Assay [PDF]

Frontiers in Endocrinology, 2017
BackgroundMonitoring blood levels of human growth hormone (hGH) in most children with short stature deficiencies is crucial for taking a decision of treatment with extended course of daily and expensive doses of recombinant hGH (rhGH or Somatropin ...
Hossam Murad, Jana Mir Assaad, Rasha Al-Shemali, Abdul Qader Abbady   +3 more
doaj   +2 more sources

Development of camelid monoclonal nanobody against SLC39A6 zinc transporter protein [PDF]

Iranian Journal of Basic Medical Sciences, 2021
Objective(s): SLC39A6 (solute carrier family 39) or LIV-1, is a zinc-transporter protein associated with estrogen-positive breast cancer and its metastatic spread.
Hajarsadat Ghaderi, Zahra Noormohammadi, Mahdi Habibi-Anbouhi, Fatemeh Kazemi-Lomedasht, Mahdi Behdani   +4 more
doaj   +1 more source

NLR immune receptor–nanobody fusions confer plant disease resistance

Science, 2023
Plant pathogens cause recurrent epidemics, threatening crop yield and global food security. Efforts to retool the plant immune system have been limited to modifying natural components and can be nullified by the emergence of new pathogen strains. Made-to-
Jiorgos Kourelis, Clémence Marchal, Andrés Posbeyikian, A. Harant, S. Kamoun   +4 more
semanticscholar   +1 more source

TROP2-directed nanobody-drug conjugate elicited potent antitumor effect in pancreatic cancer

Journal of Nanobiotechnology, 2023
Background Pancreatic cancer is a highly aggressive malignancy with limited treatment options and a poor prognosis. Trophoblast cell surface antigen 2 (TROP2), a cell surface antigen overexpressed in the tumors of more than half of pancreatic cancer ...
Cailing Xu, Min Zhu, Qian Wang, Jiajun Cui, Yuping Huang, Xiting Huang, Jing Huang, J. Gai, Guanghui Li, P. Qiao, Xian Zeng, Dianwen Ju, Yakun Wan, Xuyao Zhang   +13 more
semanticscholar   +1 more source

Screening, Expression, and Identification of Nanobody against SARS-CoV-2 Spike Protein

Cells, 2022
Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an infectious disease that has become a serious burden on global public health.
Qianling Su, Wei Shi, Xianing Huang, Yakun Wan, Guanghui Li, Bengang Xing, Zhi Ping Xu, Hongbo Liu, Bruce D. Hammock, Xiaomei Yang, Shihua Yin, Xiaoling Lu   +11 more
doaj   +1 more source

Nanobody-based chimeric antigen receptor T cells designed by CRISPR/Cas9 technology for solid tumor immunotherapy

Signal Transduction and Targeted Therapy, 2021
Chimeric antigen receptor-based T-cell immunotherapy is a promising strategy for treatment of hematological malignant tumors; however, its efficacy towards solid cancer remains challenging.
Fengzhen Mo, Siliang Duan, Xiaobing Jiang, Xiaomei Yang, Xiaoqiong Hou, Wei Shi, Cueva Jumbo Juan Carlos, Aiqun Liu, Shihua Yin, Wu Wang, Hua Yao, Zihang Yu, Zhuoran Tang, Shenxia Xie, Ziqiang Ding, Xinyue Zhao, Bruce D. Hammock, Xiaoling Lu   +17 more
doaj   +1 more source