Results 11 to 20 of about 12,492 (212)

Structure of Nanobody Nb23 [PDF]

Molecules, 2021
Background: Nanobodies, or VHHs, are derived from heavy chain-only antibodies (hcAbs) found in camelids. They overcome some of the inherent limitations of monoclonal antibodies (mAbs) and derivatives thereof, due to their smaller molecular size and higher stability, and thus present an alternative to mAbs for therapeutic use.
Percipalle M., Hunashal Y., Steyaert J., Fogolari F., Esposito G.   +4 more
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Nanobodies in cancer

Seminars in Immunology, 2021
For treatment and diagnosis of cancer, antibodies have proven their value and now serve as a first line of therapy for certain cancers. A unique class of antibody fragments called nanobodies, derived from camelid heavy chain-only antibodies, are gaining increasing acceptance as diagnostic tools and are considered also as building blocks for chimeric ...
Elisha R, Verhaar, Andrew W, Woodham, Hidde L, Ploegh   +2 more
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Special Issue: Nanobody [PDF]

Antibodies, 2020
Since their first description in 1993 [...]
Patrick Chames, Ulrich Rothbauer
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Immunogenicity Risk Profile of Nanobodies [PDF]

Frontiers in Immunology, 2021
Nanobodies (Nbs), the variable domains of camelid heavy chain-only antibodies, are a promising class of therapeutics or in vivo imaging reagents entering the clinic. They possess unique characteristics, including a minimal size, providing fast pharmacokinetics, high-target specificity, and an affinity in the (sub-)nanomolar range in conjunction with an
Chloé Ackaert, Natalia Smiejkowska, Catarina Xavier, Yann G. J. Sterckx, Yann G. J. Sterckx, Sofie Denies, Benoit Stijlemans, Benoit Stijlemans, Yvon Elkrim, Nick Devoogdt, Vicky Caveliers, Vicky Caveliers, Tony Lahoutte, Tony Lahoutte, Serge Muyldermans, Karine Breckpot, Marleen Keyaerts, Marleen Keyaerts   +17 more
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Nanobody-Targeted Photodynamic Therapy: Nanobody Production and Purification

, 2022
Nanobodies have recently been introduced to the field of photodynamic therapy (PDT) as a very promising strategy to target photosensitizers selectively to cancer cells. Nanobodies are known for their characteristic small size (15 kDa), high specificity, and high binding affinities.
Mashayekhi, Vida, Schooten, Erik, van Bergen En Henegouwen, Paul M P, Kijanka, Marta M, Oliveira, Sabrina   +4 more
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A Novel Small Molecular Antibody, HER2-Nanobody, Inhibits Tumor Proliferation in HER2-Positive Breast Cancer Cells In Vitro and In Vivo

Frontiers in Oncology, 2021
Breast cancer is the most common malignant cancer in women worldwide, especially in developing countries. Herceptin is a monoclonal antibody with an antitumor effect in HER2-positive breast cancer. However, the large molecular weight of Herceptin limited
Yan Yan, Yan Yan, Xiao Cheng, Lin Li, Rumeng Zhang, Yong Zhu, Zhengsheng Wu, Zhengsheng Wu, Keshuo Ding, Keshuo Ding   +9 more
doaj   +1 more source

Chemogenetic Control of Nanobodies [PDF]

Nature Methods, 2019
ABSTRACTWe introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows to study their role in basic biological processes, an approach that should be applicable to numerous previously ...
Farrants, Helen, Tarnawski, Miroslaw, Müller, Thorsten G., Otsuka, Shotaro, Hiblot, Julien, Koch, Birgit, Kueblbeck, Moritz, Kräusslich, Hans-Georg, Ellenberg, Jan, Johnsson, Kai   +9 more
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Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

Microbial Cell Factories, 2023
Background Ablynx NV, a subsidiary of Sanofi, has a long-standing focus on the development of Nanobody® molecules as biopharmaceuticals (Nanobody® is a registered trademark of Ablynx NV). Nanobody molecules are single variable domains, and they have been
Manu De Groeve, Bram Laukens, Peter Schotte   +2 more
doaj   +1 more source

Rapid Delivery of Nanobodies/VHHs into Living Cells via Expressing In Vitro-Transcribed mRNA

Molecular Therapy: Methods & Clinical Development, 2020
Intracellular antigen labeling and manipulation by antibodies have been long-thought goals in the field of cell research and therapy. However, a central limitation for this application is that antibodies are not able to penetrate into the cytosol of ...
Xuechen Zhou, Rui Hao, Chen Chen, Zhipeng Su, Linhong Zhao, Zhuojuan Luo, Wei Xie   +6 more
doaj   +1 more source

Nanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein–Protein Interaction Analysis, and Protein Function Exploration

Frontiers in Immunology, 2017
Over the last two decades, nanobodies or single-domain antibodies have found their way in research, diagnostics, and therapy. These antigen-binding fragments, derived from Camelid heavy chain only antibodies, possess remarkable characteristics that favor
Els Beghein, Jan Gettemans
doaj   +1 more source