Results 31 to 40 of about 19,829 (313)

A Novel Small Molecular Antibody, HER2-Nanobody, Inhibits Tumor Proliferation in HER2-Positive Breast Cancer Cells In Vitro and In Vivo

Frontiers in Oncology, 2021
Breast cancer is the most common malignant cancer in women worldwide, especially in developing countries. Herceptin is a monoclonal antibody with an antitumor effect in HER2-positive breast cancer. However, the large molecular weight of Herceptin limited
Yan Yan, Yan Yan, Xiao Cheng, Lin Li, Rumeng Zhang, Yong Zhu, Zhengsheng Wu, Zhengsheng Wu, Keshuo Ding, Keshuo Ding   +9 more
doaj   +1 more source

Specific Detection of Proteins by a Nanobody-Functionalized Nanopore Sensor

ACS Nano, 2023
Nanopores are label-free single-molecule analytical tools that show great potential for stochastic sensing of proteins. Here, we described a ClyA nanopore functionalized with different nanobodies through a 5–6 nm DNA linker at its periphery. Ty1, 2Rs15d,
Xialin Zhang, N. S. Galenkamp, N. J. van der Heide, Julián Moreno, G. Maglia, J. Kjems   +5 more
semanticscholar   +1 more source

Evaluation of [89Zr]Zr-DFO-2Rs15d Nanobody for Imaging of HER2-Positive Breast Cancer.

Molecular Pharmaceutics, 2023
One of the most aggressive forms of breast cancer involves the overexpression of human epidermal growth factor receptor 2 (HER2). HER2 is overexpressed in ∼25% of all breast cancers and is associated with increased proliferation, increased rates of ...
Maxwell Ducharme, Lucinda A Hall, Whitney Eckenroad, Shelbie J. Cingoranelli, Hailey A. Houson, Luke Jaskowski, C. Hunter, B. Larimer, S. Lapi   +8 more
semanticscholar   +1 more source

Fully synthetic platform to rapidly generate tetravalent bispecific nanobody–based immunoglobulins

Proceedings of the National Academy of Sciences of the United States of America, 2023
Significance Nanobodies are a promising class of biologics that can be used to prevent or treat viral infections. Here, we describe the production and validation of a discovery library that produces single-domain nanobodies using an engineered human ...
Laetitia E Misson Mindrebo, Hejun Liu, G. Ozorowski, Quoc Tran, J. Woehl, Irene S. Khalek, Jessica M Smith, Shawn Barman, Fangzhu Zhao, Celina Keating, Oliver Limbo, Megan Verma, Jingjia Liu, R. Stanfield, Xueyong Zhu, H. Turner, D. Sok, Po-Ssu Huang, D. Burton, A. Ward, I. Wilson, J. Jardine   +21 more
semanticscholar   +1 more source

A guide to: generation and design of nanobodies [PDF]

The FEBS Journal, 2020
A nanobody (Nb) is a registered trademark of Ablynx, referring to the single antigen‐binding domain of heavy chain‐only antibodies (HCAbs) that are circulating in Camelidae. Nbs are produced recombinantly in micro‐organisms and employed as research tools or for diagnostic and therapeutic applications.
Serge Muyldermans, Serge Muyldermans
openaire   +3 more sources

A Multivalent and Thermostable Nanobody Neutralizing SARS-CoV-2 Omicron (B.1.1.529)

International Journal of Nanomedicine, 2023
Background The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variants have risen to dominance, which contains far more mutations in the spike protein in comparison to previously reported variants, compromising the efficacy of most ...
Yuying Lu, Qianlin Li, Huahao Fan, Conghui Liao, Jingsong Zhang, Huan Hu, H. Yi, Yu-Chi Peng, Jiahai Lu, Zeliang Chen   +9 more
semanticscholar   +1 more source

Nanobody cocktails potently neutralize SARS-CoV-2 D614G N501Y variant and protect mice

Proceedings of the National Academy of Sciences of the United States of America, 2021
Significance Neutralizing antibodies are important for immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and as therapeutics for the prevention and treatment of COVID-19. We identified high-affinity nanobodies against SARS-CoV-
P. Pymm, A. Adair, L. Chan, James P. Cooney, F. Mordant, C. Allison, E. Lopez, E. Haycroft, Matthew T. O’Neill, L. L. Tan, M. Dietrich, D. Drew, M. Doerflinger, M. Dengler, Nichollas E. Scott, A. Wheatley, N. Gherardin, H. Venugopal, D. Cromer, M. Davenport, R. Pickering, D. Godfrey, D. Purcell, S. Kent, A. Chung, K. Subbarao, M. Pellegrini, A. Glukhova, W. Tham   +28 more
semanticscholar   +1 more source

Nanobody-Targeted Photodynamic Therapy: Nanobody Production and Purification

, 2022
Nanobodies have recently been introduced to the field of photodynamic therapy (PDT) as a very promising strategy to target photosensitizers selectively to cancer cells. Nanobodies are known for their characteristic small size (15 kDa), high specificity, and high binding affinities.
Mashayekhi, Vida, Schooten, Erik, van Bergen En Henegouwen, Paul M P, Kijanka, Marta M, Oliveira, Sabrina   +4 more
openaire   +4 more sources

Applications of nanobodies in brain diseases

Frontiers in Immunology, 2022
Nanobodies are antibody fragments derived from camelids, naturally endowed with properties like low molecular weight, high affinity and low immunogenicity, which contribute to their effective use as research tools, but also as diagnostic and therapeutic agents in a wide range of diseases, including brain diseases. Also, with the success of Caplacizumab,
Fang Zheng, Yucheng Pang, Luyao Li, Yuxing Pang, Jiaxin Zhang, Xinyi Wang, Geert Raes, Geert Raes   +7 more
openaire   +4 more sources

Exploring cellular biochemistry with nanobodies [PDF]

Journal of Biological Chemistry, 2020
Reagents that bind tightly and specifically to biomolecules of interest remain essential in the exploration of biology and in their ultimate application to medicine. Besides ligands for receptors of known specificity, agents commonly used for this purpose are monoclonal antibodies derived from mice, rabbits, and other animals.
Charlotte Wijne, Charlotte Wijne, Ross W. Cheloha, Ross W. Cheloha, Thomas U. Schwartz, Thibault J. Harmand, Thibault J. Harmand, Hidde L. Ploegh, Hidde L. Ploegh   +8 more
openaire   +3 more sources