Results 31 to 40 of about 12,492 (212)
Molecular Oncology, 2022 Alteration in glycosylation pattern of MUC1 mucin tandem repeats during carcinomas has been shown to negatively affect adhesive properties of malignant cells and enhance tumor invasiveness and metastasis.
Parnaz Merikhian +12 more
doaj +1 more source
Biotechnology & Biotechnological Equipment, 2021 Nanobodies belong to a new generation of antibodies produced by recombinant engineering and have high perspectives in biopharmaceutical drug formulations.
Mohamed Karam Qassas +3 more
doaj +1 more source
Molecular and Cellular Endocrinology, 2019 The family of G protein-coupled receptors (GPCRs) is the largest class of membrane proteins and an important drug target due to their role in many (patho)physiological processes. Besides small molecules, GPCRs can be targeted by biologicals including antibodies and antibody fragments.
De Groof, Timo +3 more
openaire +2 more sources
Nanobody Based Dual Specific CARs [PDF]
International Journal of Molecular Sciences, 2018 Recent clinical trials have shown that adoptive chimeric antigen receptor (CAR) T cell therapy is a very potent and possibly curative option in the treatment of B cell leukemias and lymphomas. However, targeting a single antigen may not be sufficient, and relapse due to the emergence of antigen negative leukemic cells may occur. A potential strategy to
Stijn De Munter +8 more
openaire +3 more sources
PLoS ONE, 2017 Bovine viral diarrhea-mucosal disease (BVD-MD) is caused by bovine viral diarrhea virus (BVDV), and results in abortion, stillbirth, and fetal malformation in cows. Here, we constructed the phage display vector pCANTAB 5E-VHH and then transformed it into
Tiansen Li +8 more
doaj +1 more source
Recombinant expression of nanobodies and nanobody-derived immunoreagents
Protein Expression and Purification, 2020 Antibody fragments for which the sequence is available are suitable for straightforward engineering and expression in both eukaryotic and prokaryotic systems. When produced as fusions with convenient tags, they become reagents which pair their selective binding capacity to an orthogonal function.
openaire +3 more sources
Nanobodies and Nanobody-Based Human Heavy Chain Antibodies As Antitumor Therapeutics [PDF]
Frontiers in Immunology, 2017 Monoclonal antibodies have revolutionized cancer therapy. However, delivery to tumor cells in vivo is hampered by the large size (150 kDa) of conventional antibodies. The minimal target recognition module of a conventional antibody is composed of two non-covalently associated variable domains (VH and VL).
Peter Bannas +2 more
openaire +3 more sources
FEBS Open Bio, EarlyView.Tandem VHH targeting distinct EGFR epitopes were engineered into a monovalent bispecific antibody (7D12‐EGA1‐Fc) with more potent ADCC without increasing affinity to EGFR. Structural modeling of 7D12‐EGA1‐Fc showed cross‐linking of separate EGFR domains to enhance CD16a engagement on NK cells.
Yuqiang Xu +5 more
wiley +1 more source
CDH17 nanobodies facilitate rapid imaging of gastric cancer and efficient delivery of immunotoxin
Biomaterials Research, 2022 Background It is highly desirable to develop new therapeutic strategies for gastric cancer given the low survival rate despite improvement in the past decades.
Jingbo Ma +19 more
doaj +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source