Results 31 to 40 of about 998,588 (389)

Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteria

BMC Genomics, 2023
Background Oxford Nanopore Technologies (ONT) offers an accessible platform for long-read sequencing, which improves the reconstruction of genomes and helps to resolve complex genomic contexts, especially in the case of metagenome analysis.
Mathieu Gand, Bram Bloemen, K. Vanneste, N. Roosens, S. D. De Keersmaecker   +4 more
semanticscholar   +1 more source

Direct oligonucleotide sequencing with nanopores [PDF]

Open Research Europe, 2020
Third-generation DNA sequencing has enabled sequencing of long, unamplified DNA fragments with minimal steps. Direct sequencing of ssDNA or RNA gives valuable insights like base-level modifications, phosphoramidite synthesis yield estimates and strand quality analysis, without the need to add the complimentary strand.
Sachin Chalapati, Conor A Crosbie, Dixita Limbachiya, Nimesh Pinnamaneni   +3 more
openaire   +2 more sources

Genotyping of familial Mediterranean fever gene (MEFV)—Single nucleotide polymorphism—Comparison of Nanopore with conventional Sanger sequencing

PLoS ONE, 2022
Background Through continuous innovation and improvement, Nanopore sequencing has become a powerful technology. Because of its fast processing time, low cost, and ability to generate long reads, this sequencing technique would be particularly suitable ...
Jonas Schmidt, Sandro Berghaus, Frithjof Blessing, Holger Herbeck, Josef Blessing, Peter Schierack, Stefan Rödiger, Dirk Roggenbuck, Folker Wenzel   +8 more
doaj   +2 more sources

Drug resistance and vaccine target surveillance of Plasmodium falciparum using nanopore sequencing in Ghana

Nature Microbiology, 2023
Malaria results in over 600,000 deaths annually, with the highest burden of deaths in young children living in sub-Saharan Africa. Molecular surveillance can provide important information for malaria control policies, including detection of antimalarial ...
Sophia T. Girgis, Edem Adika, Felix E. Nenyewodey, Dodzi K. Senoo Jnr, J. Ngoi, Kukua Bandoh, Oliver Lorenz, Guus van de Steeg, Alexandria J. R. Harrott, Sebastian Nsoh, Kim Judge, Richard D. Pearson, Jacob Almagro-Garcia, Samirah Saiid, Solomon Atampah, Enock K. Amoako, C. Morang’a, V. Asoala, Elrmion S. Adjei, William Burden, William Roberts-Sengier, Eleanor Drury, Megan L Pierce, S. Gonçalves, G. Awandare, Dominic P. Kwiatkowski, L. Amenga-Etego, W. Hamilton   +27 more
semanticscholar   +1 more source

Three decades of nanopore sequencing [PDF]

Nature Biotechnology, 2016
A long-held goal in sequencing has been to use a voltage-biased nanoscale pore in a membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule through that pore. With the development of enzyme-based methods that ratchet polynucleotides through the nanopore, nucleobase-by-nucleobase, measurements of changes in the current ...
David, Deamer, Mark, Akeson, Daniel, Branton   +2 more
openaire   +2 more sources

Recent advances in nanopore sequencing [PDF]

ELECTROPHORESIS, 2012
The prospect of nanopores as a next‐generation sequencing platform has been a topic of growing interest and considerable government‐sponsored research for more than a decade. Oxford Nanopore Technologies recently announced the first commercial nanopore sequencing devices, to be made available by the end of 2012, while other companies (Life, Roche, and ...
Raj D, Maitra, Jungsuk, Kim, William B, Dunbar   +2 more
openaire   +2 more sources

Nanopore DNA sequencing with MspA [PDF]

Proceedings of the National Academy of Sciences, 2010
Nanopore sequencing has the potential to become a direct, fast, and inexpensive DNA sequencing technology. The simplest form of nanopore DNA sequencing utilizes the hypothesis that individual nucleotides of single-stranded DNA passing through a nanopore will uniquely modulate an ionic current flowing through the pore, allowing the record ...
Ian M, Derrington, Tom Z, Butler, Marcus D, Collins, Elizabeth, Manrao, Mikhail, Pavlenok, Michael, Niederweis, Jens H, Gundlach   +6 more
openaire   +2 more sources

Modification mapping by nanopore sequencing

Frontiers in Genetics, 2022
Next generation sequencing (NGS) has provided biologists with an unprecedented view into biological processes and their regulation over the past 2 decades, fueling a wave of development of high throughput methods based on short read DNA and RNA sequencing. For nucleic acid modifications, NGS has been coupled with immunoprecipitation, chemical treatment,
Laura K. White, Jay R. Hesselberth
openaire   +3 more sources

Accuracy of Nanopore Sequencing as a Diagnostic Assay for Pulmonary Tuberculosis versus Smear, Culture and Xpert MTB/RIF: A Head-to-Head Comparison

Tropical Medicine and Infectious Disease, 2023
Early diagnosis of pulmonary tuberculosis (PTB) is pivotal for achieving effective tuberculosis (TB) control. This study aimed to assess the effectiveness of nanopore sequencing of sputum, bronchoalveolar lavage fluid (BALF), and pleural fluid samples ...
Juan Yang, Wei Ye, Chao Zhang, Wenhong Lin, Lin Mei, Shengsheng Liu, Jie Liu   +6 more
semanticscholar   +1 more source

Reversible positioning of single molecules inside zero-mode waveguides [PDF]

, 2014
We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to
Foquet, Mathieu, Korlach, Jonas, Larkin, Joseph, Turner, Stephen W., Wanunu, Meni   +4 more
core   +2 more sources