Results 41 to 50 of about 61,989 (298)

Graphene Nanopores for Protein Sequencing [PDF]

Biophysical Journal, 2016
An inexpensive, reliable method for protein sequencing is essential to unraveling the biological mechanisms governing cellular behavior and disease. Current protein sequencing methods suffer from limitations associated with the size of proteins that can be sequenced, the time, and the cost of the sequencing procedures. This study reports the results of
Wilson, James, Sloman, Leila, He, Zhiren, Aksimentiev, Aleksei   +3 more
openaire   +3 more sources

From squiggle to basepair: computational approaches for improving nanopore sequencing read accuracy

Genome Biology, 2018
Nanopore sequencing is a rapidly maturing technology delivering long reads in real time on a portable instrument at low cost. Not surprisingly, the community has rapidly taken up this new way of sequencing and has used it successfully for a variety of ...
Franka J. Rang, Wigard P. Kloosterman, Jeroen de Ridder   +2 more
doaj   +1 more source

NanoSTR: A method for detection of target short tandem repeats based on nanopore sequencing data

Frontiers in Molecular Biosciences, 2023
Short tandem repeats (STRs) are widely present in the human genome. Studies have confirmed that STRs are associated with more than 30 diseases, and they have also been used in forensic identification and paternity testing.
Jidong Lang, Zhihua Xu, Yue Wang, Jiguo Sun, Zhi Yang   +4 more
doaj   +1 more source

Models and information-theoretic bounds for nanopore sequencing

, 2018
Nanopore sequencing is an emerging new technology for sequencing DNA, which can read long fragments of DNA (~50,000 bases) in contrast to most current short-read sequencing technologies which can only read hundreds of bases. While nanopore sequencers can
Diggavi, Suhas, Kannan, Sreeram, Mao, Wei   +2 more
core   +1 more source

Nanopore Sequencing Technology and Tools for Genome Assembly: Computational Analysis of the Current State, Bottlenecks and Future Directions

, 2018
Nanopore sequencing technology has the potential to render other sequencing technologies obsolete with its ability to generate long reads and provide portability.
Alkan, Can, Cali, Damla Senol, Ghose, Saugata, Kim, Jeremie S., Mutlu, Onur   +4 more
core   +2 more sources

Polymer translocation through a nanopore under an applied external field [PDF]

, 2006
We investigate the dynamics of polymer translocation through a nanopore under an externally applied field using the 2D fluctuating bond model with single-segment Monte Carlo moves.
Alberts B., Chang D.-C., Darnell J., de Gennes P. G., Doi M., Guo L., Ilkka Huopaniemi, Kaifu Luo, See-Chen Ying, Tapio Ala-Nissila   +9 more
core   +2 more sources

De novo whole-genome assembly of a wild type yeast isolate using nanopore sequencing [version 2; referees: 1 approved, 3 approved with reservations]

F1000Research, 2018
Background: The introduction of the MinION sequencing device by Oxford Nanopore Technologies may greatly accelerate whole genome sequencing. Nanopore sequence data offers great potential for de novo assembly of complex genomes without using other ...
Michael Liem, Hans J. Jansen, Ron P. Dirks, Christiaan V. Henkel, G. Paul H. van Heusden, Richard J.L.F. Lemmers, Trifa Omer, Shuai Shao, Peter J. Punt, Herman P. Spaink   +9 more
doaj   +1 more source

Application of Nanopore Sequencing for High Throughput Genotyping in Horses

Animals, 2023
Nanopore sequencing is a third-generation biopolymer sequencing technique that relies on monitoring the changes in an electrical current that occur as nucleic acids are passed through a protein nanopore.
Artur Gurgul, Igor Jasielczuk, Tomasz Szmatoła, Sebastian Sawicki, Ewelina Semik-Gurgul, Bogusława Długosz, Monika Bugno-Poniewierska   +6 more
doaj   +1 more source

Detailed evaluation of data analysis tools for subtyping of bacterial isolates based on whole genome sequencing : Neisseria meningitidis as a proof of concept [PDF]

, 2019
Whole genome sequencing is increasingly recognized as the most informative approach for characterization of bacterial isolates. Success of the routine use of this technology in public health laboratories depends on the availability of well-characterized ...
Bertrand, Sophie, De Keersmaecker, Sigrid CJ, Marchal, Kathleen, Mattheus, Wesley, Roosens, Nancy HC, Saltykova, Assia   +5 more
core   +1 more source

MethPhaser: methylation-based long-read haplotype phasing of human genomes

Nature Communications
The assignment of variants across haplotypes, phasing, is crucial for predicting the consequences, interaction, and inheritance of mutations and is a key step in improving our understanding of phenotype and disease.
Yilei Fu, Sergey Aganezov, Medhat Mahmoud, John Beaulaurier, Sissel Juul, Todd J. Treangen, Fritz J. Sedlazeck   +6 more
doaj   +1 more source