Results 81 to 90 of about 137,602 (289)
Veterinary World, 2012 Aim: The present study deals with molecular technique Nested RT-PCR for detection of rabies viral RNA from biological fluid samples (Saliva, Milk and Urine) collected from animal suspected for rabies and to compare the sensitivity of Nested RT-PCR ...
M Dandale +6 more
doaj +1 more source
Hierarchical DNA Memory Based on Nested PCR [PDF]
, 2003 This paper presents a hierarchical DNA memory based on nested PCR. Each DNA strand in memory consists of address blocks and a data block. In order to access specific data, we specify the order of the address primers, and nested PCR are performed by using these primers.
Satoshi Kashiwamura +4 more
openaire +1 more source
Bioenergy Cropping Reduces the Spatiotemporal Scaling of Soil Bacterial Biodiversity
Advanced Science, EarlyView.Consistent with patterns observed in plant and animal communities, soil bacterial communities exhibit significant species–time–area and phylogenetic–time–area relationships independent of nested structure. Bioenergy cropping significantly reduces the spatiotemporal scaling rates, particularly in sandy loam soils.
Zhencheng Ye +19 more
wiley +1 more source
Malaria Journal, 2012 Background A reliable and simple test for the detection of malaria parasite is crucial in providing effective treatment and therapeutic follow-up, especially in malaria elimination programmes.
Lee Pei-Wen +6 more
doaj +1 more source
Single-tube nested PCR in the diagnosis of tuberculosis. [PDF]
Journal of Clinical Pathology, 1996 To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens.A single-tube nested PCR, utilising two sets of primers with different melting temperatures (88 degrees C for external primers; 70 degrees C for internal primers) to augment ...
Chan, KS +6 more
openaire +3 more sources
Advanced Science, EarlyView.Tumor‐derived extracellular vesicles program inflammatory lung premetastatic niches through selective delivery of long noncoding RNAs. This work reveals EVs‐associated lncOSLMT as a key driver of lung fibroblast activation via m6A‐dependent PTGS2 stabilization.
Hongbo Li +10 more
wiley +1 more source
Identification of transporter genes from the fungal endophyte Neotyphodium lolii : this thesis is presented in partial fulfilment of the requirements for the degree of Master of Science (MSc) in Plant Biology at Massey University, Palmerston North, New Zealand / [PDF]
, 2006 Neotyphodium lolii is an endophytic fungus that lives in the pasture grass, Lolium perenne. They share a mutualistic symbiotic relationship. N. lolii lives out its life cycle within the plant and produces secondary metabolites, including alkaloids ...
Knox, Deborah Sian
core
DNA Microarray Probe Preparation by Gel Isolation Nested PCR [PDF]
BMB Reports, 2004 To develop a simplified method that can rapidly prepare DNA microarray probes in a massive scale, a lambda phage genomic DNA-fragments library was constructed for the microarray-probes collection. Four methods of DNA band recovery from the first PCR products were tested and compared.
Hong-min, Wang +5 more
openaire +2 more sources
Advanced Science, EarlyView.In hypoxic microenvironment, WNT5A is predominantly secreted by tumor‐associated macrophages. Hypoxia‐induced WTAP mediates ROR1 stability by m6A modifications in a HuR‐dependent manner in Glioma stem cells (GSCs). WNT5A activates the WNT pathway via ROR1 binding on GSCs, driving glioma‐derived endothelial cells (GDECs) differentiation.
Xiaoyong Chen +15 more
wiley +1 more source
Performance of nested-RT-PCR assays for Zika and Dengue detection
Acta Scientiarum: Biological SciencesArboviruses represent a public health problem in developing countries. Accurate diagnosis has been challenging in Dengue virus endemic countries, especially since the introduction of the Zika and Chikungunya viruses.
José Henrique Francisco Roma +7 more
doaj +1 more source