Results 61 to 70 of about 186,282 (233)
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013 The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa +8 more
doaj
BMC Infectious Diseases, 2005 Background Laboratory diagnosis of Chlamydophila psittaci, an important turkey respiratory pathogen, is difficult. To facilitate the diagnosis, a nested PCR-enzyme immunoassay (PCR-EIA) was developed to detect the Cp.
Goddeeris Bruno M +5 more
doaj +1 more source
Diagnosis of canine coronavirus infection using nested-PCR
Journal of Virological Methods, 2000 The results of polymerase chain reaction (PCR) and nested polymerase chain reaction (n-PCR) assays for the diagnosis of canine coronavirus (CCV) infection, and the comparison with other diagnostic techniques, such as electron microscopy (EM) and virus isolation using A-72 cell line are reported.
Pratelli, Annamaria +5 more
openaire +2 more sources
Development of an Rt Nested PCR-Elisa Diagnostic Test for the Detection of Newcastle Disease Virus [PDF]
, 1999 Newcastle disease virus causes an economically important poultry disease known as Newcastle disease in Malaysia. The velogenic strain of this virus causes 1 00% mortality in infected chicken.
Kho, Chiew Ling
core
Advanced Science, EarlyView.A muscle‐bone endocrine pathway in aging is revealed in which extracellular vesicles released from atrophic skeletal muscle (Aged‐SKM‐EVs) inhibit bone formation. These EVs deliver miR‐125a‐5p to osteoblasts, thereby suppressing the SIRT7‐Sp7 signaling axis and osteogenic differentiation.
Xiaoyan Shao +22 more
wiley +1 more source
Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1. [PDF]
PLoS ONE, 2014 In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates.
Cristina P Araújo +14 more
doaj +1 more source
Nested PCR in magnetically actuated circular closed-loop PCR microchip system [PDF]
Microchimica Acta, 2012 We demonstrate a new and sensitive amplifica- tion technique (referred to as Nested Polymerase Chain Reaction; nPCR). It based on a magnetically actuated circu- lar closed-loop PCR microchip system. nPCR involves the use of two sets of primers in two successive PCR runs, and allows the amplification of a single locus from a minute quantity of template ...
Lok, Khoi Seng +3 more
openaire +3 more sources
Phytoplasma detection in coconut palm and other tropical crops [PDF]
, 2010 Phytoplasmas are small bacteria with very small genomes which also have extremely low levels of the nucleotides guanine plus cytosine (G+C). They are associated with hundreds of plant diseases globally.
Nejat, Naghmeh, Vadamalai, Ganesan
core
Detection of HIV-1 infection in dried blood spots from a 12-year-old ABO bedside test card [PDF]
, 1998 Background and Objectives: We tested dried blood from an ABO bedside test card which had been stored at room temperature for 12 years, to prove that a patient with HIV-1 infection had been infected by blood transfusion. Materials and Methods: Immunoblots
Eberle, J. +4 more
core +1 more source
Advanced Science, EarlyView.This study introduces Cadict, an EGFR‐targeted nanodrug that co‐delivers cuproptosis and disulfidptosis inducers to overcome immune resistance. Cadict synergistically enhances tumor cytotoxicity and sensitizes cancers to ICIs by upregulating PD‐L1 via an Eif5b‐dependent translation mechanism, fostering a potent antitumor immune response and ...
Shaoqing Huang +12 more
wiley +1 more source