Results 101 to 110 of about 3,321 (184)

Anti-Neu5Gc Antibody Kit Protocol - ELISA v4 [PDF]

open access: yes, 2019
This protocol is essentially the same as a standard ELISA protocol, with one major exception - all animal compounds must be avoided in buffers, etc. because they contain Neu5Gc, which will interfere with antibody activity and result in misleading data.
openaire   +1 more source

A systematic review reveals conflicting evidence for the prevalence of antibodies against the sialic acid ‘xenoautoantigen’ Neu5Gc in humans and the need for a standardised approach to quantification

open access: yesFrontiers in Molecular Biosciences
Despite an array of hypothesised implications for health, disease, and therapeutic development, antibodies against the non-human sialic acid N-glycolylneuraminic acid (Neu5Gc) remain a subject of much debate.
Esme Hutton   +5 more
doaj   +1 more source

Correlation between total and inhibitable anti-Neu5Gc IgG titers in the NHS cohort.

open access: yes, 2018
Plasma anti-Neu5Gc IgG levels were quantified by ELISA using wild type mouse serum that contains naturally occurring Neu5Gc-containing epitopes as the target antigen (N = 46).
Annie N. Samraj (5410286)   +15 more
core   +1 more source

Characterization of immunogenic Neu5Gc in bioprosthetic heart valves

open access: yes
Background: The two common sialic acids (Sias) in mammals are N-acetylneuraminic acid (Neu5Ac) and its hydroxylated form N-glycolylneuraminic acid (Neu5Gc). Unlike most mammals, humans cannot synthesize Neu5Gc that is considered foreign and recognized by
Cozzi, Emanuele   +14 more
core  

Variability of Sialic Acids in Beef Breeds and Nutritional Implications in Red Meat

open access: yesMolecules
This study examines the variability of sialic acids, specifically N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), in beef from seven cattle breeds (Holstein Friesian, Red Pied, Maremmana, Chianina, Charolais, Limousin, and ...
Michela Contò   +3 more
doaj   +1 more source

Assessment of Sialic Acid Content in Dairy Products from Buffalo’s and Goat’s Milk Compared to Cow’s Milk

open access: yesDairy
Sialic acids (SIAs) are bioactive compounds present in glycoproteins and glycolipids, playing key roles in human physiology. N-acetylneuraminic acid (Neu5Ac), the only sialic acid synthesized by humans, contributes to neurodevelopment and immune function,
Simona Rinaldi   +5 more
doaj   +1 more source

Anti-Neu5Gc Antibody Kit Protocol - Western Blot v3 [PDF]

open access: yes, 2019
The Anti-Neu5Gc Antibody Kit contains the essential monospecific polyclonal chicken IgY antibody, along with a negative control primary antibody to detect the presence of Neu5Gc on glycoconjugates by Western blot (WB). Samples to be evaluated are first subjected to SDS-PAGE, followed by transfer to a nitrocellulose or polyvinylidenedifluoride (PVDF ...
openaire   +1 more source

Co-localization of Neu5Gc staining with markers for endosomes and Golgi in BMD and DMD muscle.

open access: yes, 2014
(A) BMD muscle co-stained for Neu5Gc (green) and clathrin (red), a marker of endosomes. Merged image on right shows overlap of Neu5Gc and clathrin expression in yellow. Arrow marks several examples of co-staining.
Bethannie Golden (520799)   +8 more
core   +1 more source

The avian influenza A virus receptor SA-α2,3-Gal is expressed in the porcine nasal mucosa sustaining the pig as a mixing vessel for new influenza viruses

open access: yesVirus Research
Influenza A viruses (IAVs) originate from wild birds but have on several occasions jumped host barriers and are now also circulating in humans and mammals.
Charlotte Kristensen   +3 more
doaj   +1 more source

Distribution of Anti-Neu5Gc IgG titers against a mixture of epitopes in the EPIC-Norfolk cohort.

open access: yes, 2018
(A) Serum anti-Neu5Gc IgG antibodies in the EPIC-Norfolk population assayed as in Fig 1. Serum total and inhibitable anti-Neu5Gc IgG levels were quantified by ELISA using wild type mouse serum that contains naturally occurring Neu5Gc containing epitopes ...
Annie N. Samraj (5410286)   +15 more
core   +1 more source

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