Results 61 to 70 of about 9,796 (161)

Integrative miRNOMe profiling reveals the miR‐195‐5p–CHEK1 axis and its impact on luminal breast cancer outcomes

Molecular Oncology, Volume 19, Issue 11, Page 3409-3426, November 2025.
In luminal (ER+) breast carcinoma (BC), miRNA profiling identified miR‐195‐5p as a key regulator of proliferation that targets CHEK1, CDC25A, and CCNE1. High CHEK1 expression correlates with worse relapse‐free survival after chemotherapy, especially in patients with luminal A subtype.
Veronika Boušková, Marie Ehrlichová, Alžběta Spálenková, Ivona Krus, Simona Šůsová, Viktor Hlaváč, Vlasta Němcová, Renata Koževnikovová, Markéta Trnková, David Vrána, Jiří Gatěk, Kateřina Kopečková, Marcela Mrhalová, Soňa Měšťáková, Pavel Souček   +14 more
wiley   +1 more source

Testing reference genes for transcript profiling in Uromyces appendiculatus during urediospore infection of common bean.

PLoS ONE, 2020
Uromyces appendiculatus is a major pathogen on common bean. Like other rust fungi, it uses effectors to influence its host plant. Effectors are assumed to possess characteristic expression profiles, reflecting their activity during the infection process.
Tobias I Link
doaj   +1 more source

Felis catus papillomavirus type 2 infection and skin cancer in domestic cats : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatū, New Zealand [PDF]

, 2016
Felis catus papillomavirus type 2 (FcaPV-2) is a virus which commonly infects the skin of domestic cats. While most infections are asymptomatic, there is growing evidence that FcaPV-2 may play a role in the development of a subset of feline cutaneous ...
Thomson, Neroli Anne
core  

Comprehensive profiling of lncRNAs and mRNAs enriched in small extracellular vesicles for early noninvasive detection of colorectal cancer: diagnostic panel assembly and extensive validation

Molecular Oncology, Volume 19, Issue 11, Page 3445-3462, November 2025.
Small extracellular vesicles are a promising source of diagnostic molecules. We conducted a comprehensive study, including transcriptome profiling and RT‐qPCR validation on large cohorts of samples. Diagnostic panels enabling sensitive detection of colorectal cancer and precancerous lesions were established. Some molecules were differentially expressed
Petra Vychytilova‐Faltejskova, Marketa Pavlikova, Lucie Pifkova, Robin Jugas, Tana Machackova, Lenka Radova, Milana Sachlova, Renata Bartosova, Tetiana Samoilenko, Zuzana Feckova, Jana Orlickova, Erika Slamova, Elleni Ponechal Michu, Dagmar Al Tukmachi, Michaela Ruckova, Martina Vodinska, Jan Kotoucek, Tina Catela Ivkovic, Marie Boudna, Lucia Bohovicova, Teodor Stanek, Jana Halamkova, Martin Svoboda, Vladimir Prochazka, Tomas Grolich, Zdenek Kala, Ondrej Slaby   +26 more
wiley   +1 more source

18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells [PDF]

, 2012
Background: One requisite of quantitative reverse transcription PCR (qRT-PCR) is to normalise the data with an internal reference gene that is invariant regardless of treatment, such as virus infection.
Baquero Perez, Belinda, Brookes, Sharon M., Brown, Ian H., Chang, Kin-Chow, Dunham, Stephen P., Kuchipudi, Suresh V., Nelli, Rahul K., Sebastian, Sujith, Slomka, Marek J., Tellabati, Meenu, White, Gavin A.   +10 more
core   +1 more source

A transcriptomic atlas facilitating systems biology approaches in pea

Crop Science, Volume 65, Issue 6, November/December 2025.
Abstract Pea is a model organism in biology and an agronomic crop, entering the genomics era. Although RNA‐sequencing (RNA‐seq) has become the main transcriptomic approach in biology, there is currently no genome‐wide expression atlas covering a wide range of biological conditions for this species.
Gatepe Cedoine Kodjovi, Mathias Coulon, Lisa Couturier, Amélie Morin, Ingrid Goma‐Louamba, Caroline Artault, Guillaume Tcherkez, Cécile Vriet, Sylvain La Camera, Nathalie Pourtau, Bouziane Moumen, Joan Doidy   +11 more
wiley   +1 more source

Identifying the most suitable endogenous control for determining gene expression in hearts from organ donors [PDF]

, 2013
BACKGROUND: Quantitative real-time reverse transcription PCR (qRT-PCR) is a useful tool for assessing gene expression in different tissues, but the choice of adequate controls is critical to normalise the results, thereby avoiding differences and ...
Astudillo, Aurora, Escudero, Dolores, Gómez, Enrique, Otero, Jesús, Pérez, Silvia, Rodríguez, Aida, Royo, L.J. (Luis), Álvarez, Francisco   +7 more
core  

Lactate Promotes the Second Cell Fate Decision in Blastocysts by Prompting Primitive Endoderm Formation Through an Intercellular Positive Feedback Loop That Couples Paracrine FGF Signalling

Cell Proliferation, Volume 58, Issue 10, October 2025.
Paracrine FGF4 from EPI precursor cells promotes lactate synthesis within PrE precursor cells, while the increased lactate in turn stimulates FGF‐ERK signalling via lactate shuttling. The increased lactate in PrE precursor cells directly promotes PrE formation by elevating the level of histone lactylation. ABSTRACT Lactate has been widely recognised as
Xiao Hu, Yawen Tang, Wei Zhao, Juan Liu, Zhize Liu, Qianyin Yang, Meiqiang Chu, Jianhui Tian, Lei An, Shumin Wang   +9 more
wiley   +1 more source

Selection and validation of appropriate reference genes for RT–qPCR analysis of Nitraria sibirica under various abiotic stresses

BMC Plant Biology, 2022
Background Nitraria sibirica Pall. is a halophytic shrub with strong environmental adaptability that can survive in extremely saline-alkali and drought-impacted environments. Gene expression analysis aids in the exploration of the molecular mechanisms of
Aishuang Hu, Xiuyan Yang, Jianfeng Zhu, Xiuping Wang, Jiaxin Liu, Jiping Wang, Haiwen Wu, Huilong Zhang, Huaxin Zhang   +8 more
doaj   +1 more source

Identification of stably expressed reference small non-coding RNAs for microRNA quantification in high-grade serous ovarian carcinoma tissues [PDF]

, 2016
MicroRNAs (miRNAs) belong to a family of small non‐coding RNAs (sncRNAs) playing important roles in human carcinogenesis. Multiple investigations reported miRNAs aberrantly expressed in several cancers, including high‐grade serous ovarian carcinoma (HGS ...
Bandiera, Elisabetta, Bignotti, Eliana, Calza, Stefano, Odicino, Franco., Ravaggi, Antonella, Romani, Chiara, Sartori, Enrico, Tassi, Renata A., Todeschini, Paola, Zanotti, Laura   +9 more
core   +1 more source