Results 71 to 80 of about 9,815 (163)

Selection and validation of appropriate reference genes for RT–qPCR analysis of Nitraria sibirica under various abiotic stresses

BMC Plant Biology, 2022
Background Nitraria sibirica Pall. is a halophytic shrub with strong environmental adaptability that can survive in extremely saline-alkali and drought-impacted environments. Gene expression analysis aids in the exploration of the molecular mechanisms of
Aishuang Hu, Xiuyan Yang, Jianfeng Zhu, Xiuping Wang, Jiaxin Liu, Jiping Wang, Haiwen Wu, Huilong Zhang, Huaxin Zhang   +8 more
doaj   +1 more source

Comprehensive profiling of lncRNAs and mRNAs enriched in small extracellular vesicles for early noninvasive detection of colorectal cancer: diagnostic panel assembly and extensive validation

Molecular Oncology, Volume 19, Issue 11, Page 3445-3462, November 2025.
Small extracellular vesicles are a promising source of diagnostic molecules. We conducted a comprehensive study, including transcriptome profiling and RT‐qPCR validation on large cohorts of samples. Diagnostic panels enabling sensitive detection of colorectal cancer and precancerous lesions were established. Some molecules were differentially expressed
Petra Vychytilova‐Faltejskova, Marketa Pavlikova, Lucie Pifkova, Robin Jugas, Tana Machackova, Lenka Radova, Milana Sachlova, Renata Bartosova, Tetiana Samoilenko, Zuzana Feckova, Jana Orlickova, Erika Slamova, Elleni Ponechal Michu, Dagmar Al Tukmachi, Michaela Ruckova, Martina Vodinska, Jan Kotoucek, Tina Catela Ivkovic, Marie Boudna, Lucia Bohovicova, Teodor Stanek, Jana Halamkova, Martin Svoboda, Vladimir Prochazka, Tomas Grolich, Zdenek Kala, Ondrej Slaby   +26 more
wiley   +1 more source

Leukocyte count affects expression of reference genes in canine whole blood samples

BMC Research Notes, 2011
Background The dog is frequently used as a model for hematologic human diseases. In this study the suitability of nine potential reference genes for quantitative RT-PCR studies in canine whole blood was investigated.
Dekker Aldo, Rothuizen Jan, Brinkhof Bas, Piek Christine J, Penning Louis C   +4 more
doaj   +1 more source

Geometric Mean of 5S rRNA and MiR-16 As A Suitable Normalizer In Esophageal Cancer [PDF]

Journal of Cell and Molecular Research, 2016
Esophageal squamous cell carcinoma (ESCC) is a deadly cancer with poor prognosis. In this regard, early diagnosis is of vital importance to cure the tumor in its early stages.
Samaneh Khazaei, Sedigheh Gharbi, Seyed Javad Mowla   +2 more
doaj   +1 more source

A transcriptomic atlas facilitating systems biology approaches in pea

Crop Science, Volume 65, Issue 6, November/December 2025.
Abstract Pea is a model organism in biology and an agronomic crop, entering the genomics era. Although RNA‐sequencing (RNA‐seq) has become the main transcriptomic approach in biology, there is currently no genome‐wide expression atlas covering a wide range of biological conditions for this species.
Gatepe Cedoine Kodjovi, Mathias Coulon, Lisa Couturier, Amélie Morin, Ingrid Goma‐Louamba, Caroline Artault, Guillaume Tcherkez, Cécile Vriet, Sylvain La Camera, Nathalie Pourtau, Bouziane Moumen, Joan Doidy   +11 more
wiley   +1 more source

Optimal use of statistical methods to validate reference gene stability in longitudinal studies.

PLoS ONE, 2019
Multiple statistical approaches have been proposed to validate reference genes in qPCR assays. However, conflicting results from these statistical methods pose a major hurdle in the choice of the best reference genes. Recent studies have proposed the use
Venkat Krishnan Sundaram, Nirmal Kumar Sampathkumar, Charbel Massaad, Julien Grenier   +3 more
doaj   +1 more source

Identification of stably expressed Internal Control Genes (ICGs) for normalization of expression data in liver of C57BL/6 mice injected with beta casomorphins.

PLoS ONE, 2023
In recent years, beta-casomorphin peptides (BCM7/BCM9) derived from the digestion of cow milk have drawn a lot of attention world over because of their proposed impact on human health.
Anurag Kumar, Monika Sodhi, Manishi Mukesh, Amandeep Kaur, Gaurav Bhakri, Vipul Chaudhary, Preeti Swami, Vishal Sharma, Ashok Kumar Mohanty, Ranjit S Kataria   +9 more
doaj   +1 more source

Lactate Promotes the Second Cell Fate Decision in Blastocysts by Prompting Primitive Endoderm Formation Through an Intercellular Positive Feedback Loop That Couples Paracrine FGF Signalling

Cell Proliferation, Volume 58, Issue 10, October 2025.
Paracrine FGF4 from EPI precursor cells promotes lactate synthesis within PrE precursor cells, while the increased lactate in turn stimulates FGF‐ERK signalling via lactate shuttling. The increased lactate in PrE precursor cells directly promotes PrE formation by elevating the level of histone lactylation. ABSTRACT Lactate has been widely recognised as
Xiao Hu, Yawen Tang, Wei Zhao, Juan Liu, Zhize Liu, Qianyin Yang, Meiqiang Chu, Jianhui Tian, Lei An, Shumin Wang   +9 more
wiley   +1 more source

Endogenous gene selection for relative quantification PCR and IL6 transcript levels in the PBMC’s of severe and non-severe dengue cases

BMC Research Notes, 2018
Objectives Dengue viral infection ranges from dengue fever to dengue haemorrhagic fever and lethal dengue shock syndrome. Currently no means are available to monitor the progression of disease.
Vigneshwari Easwar Kumar, Cleetus Cherupanakkal, Minna Catherine, Tamilarasu Kadhiravan, Narayanan Parameswaran, Soundravally Rajendiran, Agieshkumar Balakrishna Pillai   +6 more
doaj   +1 more source

Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR [PDF]

, 2009
Background: Considering the broad variation in the expression of housekeeping genes among tissues and experimental situations, studies using quantitative RT-PCR require strict definition of adequate endogenous controls.
Valeria Valente, Silvia A Teixeira, Luciano Neder, Oswaldo K Okamoto, Sueli M Oba-Shinjo, Suely KN Marie, Carlos A Scrideli, Maria L Paco-Larson, Carlos G Carlotti   +8 more
core   +2 more sources