Results 71 to 80 of about 9,796 (161)

Beet Chlorosis Virus Infection Mitigates Aphid‐Induced Plant Defences and Improves Plant Acceptability to Aphid Vectors

open access: yesMolecular Ecology, Volume 34, Issue 20, October 2025.
ABSTRACT Plant viruses often alter host traits in ways that affect interactions with herbivores, potentially facilitating their own acquisition and transmission by insect vectors. However, little is known about the molecular mechanisms underlying this phenomenon.
Thomas Armand   +5 more
wiley   +1 more source

Leukocyte count affects expression of reference genes in canine whole blood samples

open access: yesBMC Research Notes, 2011
Background The dog is frequently used as a model for hematologic human diseases. In this study the suitability of nine potential reference genes for quantitative RT-PCR studies in canine whole blood was investigated.
Dekker Aldo   +4 more
doaj   +1 more source

Geometric Mean of 5S rRNA and MiR-16 As A Suitable Normalizer In Esophageal Cancer [PDF]

open access: yesJournal of Cell and Molecular Research, 2016
Esophageal squamous cell carcinoma (ESCC) is a deadly cancer with poor prognosis. In this regard, early diagnosis is of vital importance to cure the tumor in its early stages.
Samaneh Khazaei   +2 more
doaj   +1 more source

Epithelial Memory After Respiratory Viral Infection in Mice Results in Prolonged Enhancement of Antigen Presentation

open access: yesAllergy, Volume 80, Issue 9, Page 2501-2518, September 2025.
RSV infection induces lasting epigenetic and transcriptional changes in LECs, upregulating MHC‐I and MHC‐II, further amplified by LPS exposure. This is accompanied by increased antigen uptake, processing, and presentation. These persistent alterations may influence immune responses, potentially impacting both immunity to infection and respiratory ...
Piotr P. Janas   +11 more
wiley   +1 more source

Optimal use of statistical methods to validate reference gene stability in longitudinal studies.

open access: yesPLoS ONE, 2019
Multiple statistical approaches have been proposed to validate reference genes in qPCR assays. However, conflicting results from these statistical methods pose a major hurdle in the choice of the best reference genes. Recent studies have proposed the use
Venkat Krishnan Sundaram   +3 more
doaj   +1 more source

Identification of stably expressed Internal Control Genes (ICGs) for normalization of expression data in liver of C57BL/6 mice injected with beta casomorphins.

open access: yesPLoS ONE, 2023
In recent years, beta-casomorphin peptides (BCM7/BCM9) derived from the digestion of cow milk have drawn a lot of attention world over because of their proposed impact on human health.
Anurag Kumar   +9 more
doaj   +1 more source

microRNAs for qPCR Normalization Under Morphofunctional Conditions in Bovine Sperm (Bos taurus)

open access: yesMolecular Reproduction and Development, Volume 92, Issue 8, August 2025.
Identification of the most stable microRNAs in Bos taurus semen for qPCR analysis under sperm motility and morphology conditions. ABSTRACT Cattle represents one of the most common and widely distributed categories of large ruminants, with well‐established production practices.
Lucas Petitemberte de Souza   +14 more
wiley   +1 more source

Endogenous gene selection for relative quantification PCR and IL6 transcript levels in the PBMC’s of severe and non-severe dengue cases

open access: yesBMC Research Notes, 2018
Objectives Dengue viral infection ranges from dengue fever to dengue haemorrhagic fever and lethal dengue shock syndrome. Currently no means are available to monitor the progression of disease.
Vigneshwari Easwar Kumar   +6 more
doaj   +1 more source

Selection and validation of reference genes for real-time quantitative PCR in hyperaccumulating ecotype of Sedum alfredii under different heavy metals stresses. [PDF]

open access: yesPLoS ONE, 2013
Real-time Quantitative PCR (RT-qPCR) has become an effective method for accurate analysis of gene expression in several biological systems as well as under different experimental conditions.
Jian Sang   +5 more
doaj   +1 more source

Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies [PDF]

open access: yes, 2019
Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results.
Martel, An   +2 more
core   +1 more source

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