Proteins Connecting the Nuclear Pore Complex with the Nuclear Interior [PDF]
The Journal of Cell Biology, 1999 While much has been learned in recent years about the movement of soluble transport factors across the nuclear pore complex (NPC), comparatively little is known about intranuclear trafficking. We isolated the previously identified Saccharomyces protein Mlp1p (myosin-like protein) by an assay designed to find nuclear envelope (NE) associated proteins ...
C, Strambio-de-Castillia +2 more
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Expression of DNAJB12 or DNAJB14 causes coordinate invasion of the nucleus by membranes associated with a novel nuclear pore structure. [PDF]
PLoS ONE, 2014 DNAJB12 and DNAJB14 are transmembrane proteins in the endoplasmic reticulum (ER) that serve as co-chaperones for Hsc70/Hsp70 heat shock proteins. We demonstrate that over-expression of DNAJB12 or DNAJB14 causes the formation of elaborate membranous ...
Edward C Goodwin +4 more
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The Nuclear Pore Complex Is a Key Target of Viral Proteases to Promote Viral Replication
Viruses, 2021 Various viruses alter nuclear pore complex (NPC) integrity to access the nuclear content favoring their replication. Alteration of the nuclear pore complex has been observed not only in viruses that replicate in the nucleus but also in viruses with a ...
Luis Adrián De Jesús-González +7 more
doaj +1 more source
Types of nuclear localization signals and mechanisms of protein import into the nucleus
Cell Communication and Signaling, 2021 Nuclear localization signals (NLS) are generally short peptides that act as a signal fragment that mediates the transport of proteins from the cytoplasm into the nucleus.
Juane Lu +6 more
doaj +1 more source
Systematic Protein–Protein Interaction Analysis Reveals Intersubcomplex Contacts in the Nuclear Pore Complex [PDF]
Molecular & Cellular Proteomics, 2016 The nuclear pore complex (NPC) enables transport across the nuclear envelope. It is one of the largest multiprotein assemblies in the cell, built from about 30 proteins called nucleoporins (Nups), organized into distinct subcomplexes. Structure determination of the NPC is a major research goal.
Luise Apelt +5 more
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Biochemical characterization of nuclear pore complex protein gp210 oligomers [PDF]
European Journal of Biochemistry, 2001 The membrane‐spanning glycoprotein gp210 is a major component of the nuclear pore complex. This nucleoporin contains a large cisternal N‐terminal domain, a short C‐terminal cytoplasmic tail, and a single transmembrane segment. We show here that dimers of native gp210 can be isolated from cell extracts by immunoprecipitation, and from purified rat liver
C, Favreau +4 more
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Structure-function mapping of a heptameric module in the nuclear pore complex. [PDF]
, 2012 The nuclear pore complex (NPC) is a multiprotein assembly that serves as the sole mediator of nucleocytoplasmic exchange in eukaryotic cells. In this paper, we use an integrative approach to determine the structure of an essential component of the yeast ...
Chait, Brian T +10 more
core +2 more sources
RanBP2-Mediated SUMOylation Promotes Human DNA Polymerase Lambda Nuclear Localization and DNA Repair [PDF]
, 2020 Cellular DNA is under constant attack by a wide variety of agents, both endogenous and exogenous. To counteract DNA damage, human cells have a large collection of DNA repair factors. Among them, DNA polymerase lambda (Polλ) stands out for its versatility,
Cortés Ledesma, Felipe +4 more
core +1 more source
Herpesvirus Nuclear Egress across the Outer Nuclear Membrane
Viruses, 2021 Herpesvirus capsids are assembled in the nucleus and undergo a two-step process to cross the nuclear envelope. Capsids bud into the inner nuclear membrane (INM) aided by the nuclear egress complex (NEC) proteins UL31/34.
Richard J. Roller, David C. Johnson
doaj +1 more source
Mitogen activated protein kinase at the nuclear pore complex [PDF]
Journal of Cellular and Molecular Medicine, 2011 Mitogen activated protein (MAP) kinases control eukaryotic proliferation, and import of kinases into the nucleus through the nuclear pore complex (NPC) can influence gene expression to affect cellular growth, cell viability and homeostatic function. The NPC is a critical regulatory checkpoint for nucleocytoplasmic traffic that regulates gene expression
Faustino, Randolph S +2 more
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