Results 71 to 80 of about 8,158 (226)

Organ Specificity and Commonality of Epigenetic Aging in Low‐ and High‐Running Capacity Rats

Aging Cell, EarlyView.
Cardiorespiratory fitness (CRF) is not associated with epigenetic clocks, including newly developed ones. On the other hand, CRF is associated with global methylation profiles and promoter methylation in organ‐specific and organ‐common manner. ABSTRACT Epigenetic drift, which is gradual age‐related changes in DNA methylation patterns, plays a ...
Takuji Kawamura, Csaba Kerepesi, Juliet Polok Sarkar, Ferenc Torma, Zoltan Bori, Lei Zhou, Peter Bakonyi, Attila Kolonics, Laszlo Balogh, Mitsuru Higuchi, Vivien Pillár, Karolina Pircs, Lauren Gerard Koch, Steven Loyal Britton, Erika Koltai, Zsolt Radak   +15 more
wiley   +1 more source

A cellular model of TDP‐43 induces phosphorylated TDP‐43 aggregation with distinct changes in solubility and autophagy dysregulation

The FEBS Journal, EarlyView.
TDP‐43 protein plays a pathological role in sporadic and familial amyotrophic lateral sclerosis (ALS). Here, we developed a cellular model overexpressing TDP‐43 with three mutations linked to familial ALS, termed ‘3X‐TDP‐43’. Mutant 3X‐TDP‐43 expression showed deficits in autophagy flux and colocalization with stress granules.
Matthew B. Dopler, Muhammad I. Abeer, Sanaz Arezoumandan, Keyshawn Cox, Tyler L. Petersen, Esther H. Daniel, Carlton L. Cannon III, Angelica Bautista, Kennedy D. Blancher, Alysia M. Bland, Kylie J. Bond, Dominque A. Davis, Jessica M. Francois, Eliana J. McCray, Justin M. Morgan, Jessica L. Pulliam, Zymir A. Robinson, Mykia J. Taylor, James A. Dowell, Nigel J. Cairns, Michael A. Gitcho   +20 more
wiley   +1 more source

Two structurally distinct domains of the nucleoporin Nup170 cooperate to tether a subset of nucleoporins to nuclear pores [PDF]

Journal of Cell Biology, 2009
How individual nucleoporins (Nups) perform their role in nuclear pore structure and function is largely unknown. In this study, we examined the structure of purified Nup170 to obtain clues about its function. We show that Nup170 adopts a crescent moon shape with two structurally distinct and separable domains, a β-propeller N terminus and an α-solenoid
Flemming, Dirk, Sarges, Phillip, Stelter, Philipp, Hellwig, Andrea, Boettcher, Bettina, Hurt, Ed   +5 more
openaire   +4 more sources

Redox-Sensitive Cysteines Confer Proximal Control of the Molecular Crowding Barrier in the Nuclear Pore

Cell Reports, 2020
Summary: The nuclear pore complex forms a highly crowded selective barrier with intrinsically disordered regions at the nuclear membrane to coordinate nucleocytoplasmic molecular communications.
Wanzhen Zhang, Ryuji Watanabe, Hide A. Konishi, Takahiro Fujiwara, Shige H. Yoshimura, Masahiro Kumeta   +5 more
doaj  

A CRISPR‐Cas9‐based system for the dose‐dependent study of DNA double‐strand break sensing and repair

The FEBS Journal, EarlyView.
Using CRISPR‐Cas9, we engineered a genetic system allowing the dose‐dependent induction of a controllable number of DNA double‐strand breaks in Saccharomyces cerevisiae. The tool was used to study the kinetics of DNA break sensing and repair, including the spatial distribution of Tel1ATM kinase, which initiates the DNA damage response.
Morgane Auboiron, Jocelyn Coiffard, Sylvain Kumanski, Olivier Santt, Benjamin Pardo, María Moriel‐Carretero   +5 more
wiley   +1 more source

In vivo modeling of lethal congenital contracture syndrome 1 suggests pathomechanisms in cellular stress responses

The FEBS Journal, EarlyView.
Gle1 knockout mice fail to segregate cell lineages at the blastocyst stage, resulting in very early embryonic lethality. Gle1 knock‐in (KI) mice harboring a pathogenic variant giving rise to lethal congenital contracture syndrome 1 show both known and novel innervation defects, supportive of multiorgan pathology in human fetuses.
Tomáš Zárybnický, Sonja Lindfors, Saana Metso, Julia Koivula, Zoltan Szabo, Rasmus Valtonen, Mikko Tulppo, Johanna Magga, Samu Saarimäki, Sonja Bläuer, Ilkka Miinalainen, Risto Kerkelä, Petteri. T. Piepponen, Vootele Voikar, Juho Väänänen, Riikka Kivelä, Bhagwan Yadav, Hanna Lindgren, Pirkko Mattila, Fu‐Ping Zhang, Petra Sipilä, Reetta Hinttala, Satu Kuure   +22 more
wiley   +1 more source

Complex Commingling: Nucleoporins and the Spindle Assembly Checkpoint [PDF]

Cells, 2015
The segregation of the chromosomes during mitosis is an important process, in which the replicated DNA content is properly allocated into two daughter cells. To ensure their genomic integrity, cells present an essential surveillance mechanism known as the spindle assembly checkpoint (SAC), which monitors the bipolar attachment of the mitotic spindle ...
Ikram Mossaid, Birthe Fahrenkrog
openaire   +5 more sources

Cell type‐specific nuclear pores: a case in point for context‐dependent stoichiometry of molecular machines

Molecular Systems Biology, 2013
To understand the structure and function of large molecular machines, accurate knowledge of their stoichiometry is essential. In this study, we developed an integrated targeted proteomics and super‐resolution microscopy approach to determine the absolute
Alessandro Ori, Niccolò Banterle, Murat Iskar, Amparo Andrés‐Pons, Claudia Escher, Huy Khanh Bui, Lenore Sparks, Victor Solis‐Mezarino, Oliver Rinner, Peer Bork, Edward A Lemke, Martin Beck   +11 more
doaj   +1 more source

Genotyping by Amplicon Sequencing (GBAS) With Newly Developed SSR and EPIC Markers Reveals Structure in Populations of the Green Toad (Bufotes viridis) Across Rural and Urban Environments

Ecology and Evolution, Volume 15, Issue 7, July 2025.
We developed and tested SSR and EPIC markers for the green toad (Bufotes viridis), demonstrating their effectiveness using genotyping by high‐throughput amplicon sequencing (GBAS) across urban and rural populations. EPIC markers exhibited fewer null alleles and provided more ecologically coherent clustering results, while SSRs reflected drift‐induced ...
Vincent Kendlbacher, Yoko Philipina Krenn, Thapasya Vijayan, Christina Rupprecht, Magdalena Spießberger, Manuel Curto, Lukas Landler, Harald Meimberg   +7 more
wiley   +1 more source

Evolution of a transcriptional regulator from a transmembrane nucleoporin [PDF]

Genes & Development, 2016
Nuclear pore complexes (NPCs) emerged as nuclear transport channels in eukaryotic cells ∼1.5 billion years ago. While the primary role of NPCs is to regulate nucleo–cytoplasmic transport, recent research suggests that certain NPC proteins have additionally acquired the role of affecting gene expression at the nuclear periphery and in the nucleoplasm in
Franks, Tobias M, Benner, Chris, Narvaiza, Iñigo, Marchetto, Maria CN, Young, Janet M, Malik, Harmit S, Gage, Fred H, Hetzer, Martin W   +7 more
openaire   +5 more sources