Results 11 to 20 of about 3,075 (166)

Feedback Inhibition of Bacterial Nucleotidyltransferases by Rare Nucleotide l‑Sugars Restricts Substrate Promiscuity

, 2023
Bacterial glycomes are rich in prokaryote-specific or “rare” sugars that are absent in mammals. Like common sugars found across organisms, rare sugars are typically activated as nucleoside diphosphate sugars (NDP-sugars) by nucleotidyltransferases.
Maggie C. Zheng, Meng Zheng (1747132), Hanee Kim, Hanee Kim (16041674), Tania J. Lupoli, Tania J. Lupoli (1281309), Maggie C. Zheng (16041671), Meng Zheng   +7 more
core   +1 more source

On the stability of nucleoside diphosphate glucose metabolites: implications for studies of plant carbohydrate metabolism [PDF]

, 2017
Nucleoside diphosphate sugars (NDP-sugars) are the substrates for biosynthesis of oligo- and polysaccharides, such as starch and cellulose, and are also required for biosynthesis of nucleotides, ascorbic acid, several cofactors, glycoproteins and many ...
Ballicora, M., Alberto A Iglesias, Figueroa, C., Lunn, J., Benjamin L Hill, Iglesias, A., Miguel A Ballicora, Lunn, John E., Hill, B., Ballicora, Miguel A., Matías D Asencion Diez, John E Lunn, Asencion Diez, M., Figueroa, Carlos Maria, Carlos M Figueroa, Asención Diez, Matías Damián, Iglesias, Alberto Alvaro, Hill, Benjamin L.   +17 more
core   +1 more source

Expanding the Substrate Scope of a Bacterial Nucleotidyltransferase via Allosteric Mutations

, 2022
Bacterial glycoconjugates, such as cell surface polysaccharides and glycoproteins, play important roles in cellular interactions and survival. Enzymes called nucleotidyltransferases use sugar-1-phosphates and nucleoside triphosphates (NTPs) to produce ...
Meng Zheng (1747132), Maggie Zheng (5586443), Tania J. Lupoli (1281309)   +2 more
core   +1 more source

Nucleotide sugar dehydratases : structure, mechanism, substrate specificity, and application potential

, 2022
Nucleotide sugar (NS) dehydratases play a central role in the biosynthesis of deoxy and amino sugars, which are involved in a variety of biological functions in all domains of life.
Vogel, Ulrike, Beerens, Koen, Desmet, Tom   +2 more
core   +1 more source

Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls

, 2007
Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid.
Tenhaken, Raimund, Klinghammer, Michaela
core   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates

Biotechnology and Bioengineering, EarlyView.
Damm et al. report standardized enzymatic cascades for production of all canonical nucleoside triphosphates from the corresponding nucleosides, using kinase‐catalyzed phosphorylation from acetyl phosphate. Reactions afforded ∼ 100 mM product in near‐quantitative conversion.
Oliver T. Damm, Martin Pfeiffer, Johannes Zöhrer, Bernd Nidetzky   +3 more
wiley   +1 more source

The plant Nudix hydrolase family. [PDF]

, 2008
Nudix hydrolases are a family of proteins defined by a conserved amino-acid sequence GX(5)-EX(7)REUXEEXGU, where U is a hydrophobic residue. These enzymes are widely distributed among all classes of organisms and catalyze, with varying degrees of ...
Kraszewska, Elzbieta
core  

Adaptive Genomic Divergence Across Altitudes in Capsella bursa‐pastoris

Biological Diversity, EarlyView.
Genomic divergence was observed between high‐altitude and low‐altitude populations of Capsella bursa‐pastoris in China, consistent with an important role of ecological factors. Candidate adaptive loci associated with enhanced energy metabolism, photoprotection, and growth plasticity under altitudinal stress were identified.
Lu Liu, Jia Li, Xin Liu, Xue‐Jun Ge, Hui‐Run Huang   +4 more
wiley   +1 more source

Nucleotide- and nucleoside-converting ectoenzymes: Important modulators of purinergic signalling cascade

, 2008
The involvement of extracellular nucleotides and adenosine in an array of cell-specific responses has long been known and appreciated, but the integrative view of purinergic signalling as a multistep coordinated cascade has emerged recently.
Yegutkin, Gennady G., Yegutkin GG
core   +1 more source