Structure of nucleosome-bound human PBAF complex
bioRxiv, 2022 BAF and PBAF are mammalian SWI/SNF family chromatin remodeling complexes that possess multiple histone/DNA-binding subunits and create nucleosome-depleted/free regions for transcription activation. Despite structural studies of nucleosome-bound human BAF
Li Wang +8 more
openalex +2 more sources
Analysis of nucleosome positioning landscapes enables gene discovery in the human malaria parasite Plasmodium falciparum. [PDF]
, 2015 BackgroundPlasmodium falciparum, the deadliest malaria-causing parasite, has an extremely AT-rich (80.7 %) genome. Because of high AT-content, sequence-based annotation of genes and functional elements remains challenging.
Bunnik, Evelien M +5 more
core +4 more sources
FACT, the Bur kinase pathway, and the histone co-repressor HirC have overlapping nucleosome-related roles in yeast transcription elongation. [PDF]
PLoS ONE, 2011 Gene transcription is constrained by the nucleosomal nature of chromosomal DNA. This nucleosomal barrier is modulated by FACT, a conserved histone-binding heterodimer.
Jennifer R Stevens +6 more
doaj +3 more sources
Nucleosome wrapping energy in CpG islands and the role of epigenetic base modifications. [PDF]
ElifeGiniūnaitė R +4 more
europepmc +2 more sources
Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT
Science, 2022 During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin, but the mechanism has remained elusive. Using cryo–electron microscopy, we obtained structures of the RNAPII elongation complex (EC) passing through a nucleosome in
H. Ehara +4 more
semanticscholar +1 more source
Structural Transition of the Nucleosome during Transcription Elongation
Cells, 2023 In eukaryotes, genomic DNA is tightly wrapped in chromatin. The nucleosome is a basic unit of chromatin, but acts as a barrier to transcription. To overcome this impediment, the RNA polymerase II elongation complex disassembles the nucleosome during ...
Tomoya Kujirai +3 more
doaj +1 more source
Predicting human nucleosome occupancy from primary sequence. [PDF]
PLoS Computational Biology, 2008 Nucleosomes are the fundamental repeating unit of chromatin and comprise the structural building blocks of the living eukaryotic genome. Micrococcal nuclease (MNase) has long been used to delineate nucleosomal organization.
Shobhit Gupta +5 more
doaj +1 more source
H3.3-H4 tetramer splitting events feature cell-type specific enhancers. [PDF]
PLoS Genetics, 2013 Previously, we reported that little canonical (H3.1-H4)(2) tetramers split to form "hybrid" tetramers consisted of old and new H3.1-H(4) dimers, but approximately 10% of (H3.3-H4)2 tetramers split during each cell cycle.
Chang Huang +7 more
doaj +1 more source
Histone H1 binding to nucleosome arrays depends on linker DNA length and trajectory
Nature Structural & Molecular Biology, 2022 Throughout the genome, nucleosomes often form regular arrays that differ in nucleosome repeat length (NRL), occupancy of linker histone H1 and transcriptional activity. Here, we report cryo-EM structures of human H1-containing tetranucleosome arrays with
Marco Dombrowski +4 more
semanticscholar +1 more source
Structural basis of nucleosome retention during transcription elongation
Science, 2022 In eukaryotes, RNA polymerase (Pol) II transcribes chromatin and must move past nucleosomes, often resulting in nucleosome displacement. How Pol II unwraps the DNA from nucleosomes to allow transcription and how DNA rewraps to retain nucleosomes has been
Martin Filipovski +3 more
semanticscholar +1 more source