Results 51 to 60 of about 70,917 (227)
Cell cycle-specified fluctuation of nucleosome occupancy at gene promoters.
PLoS Genetics, 2006 The packaging of DNA into nucleosomes influences the accessibility of underlying regulatory information. Nucleosome occupancy and positioning are best characterized in the budding yeast Saccharomyces cerevisiae, albeit in asynchronous cell populations or
Gregory J Hogan +2 more
doaj +1 more source
Characteristic H3 N-tail dynamics in the nucleosome core particle, nucleosome, and chromatosome
iScience, 2022 Summary: The nucleosome core particle (NCP) comprises a histone octamer, wrapped around by ∼146-bp DNA, while the nucleosome additionally contains linker DNA.
Ayako Furukawa +7 more
doaj +1 more source
Embedded CRISPRi Enhances Gene‐Silencing Efficiency in Drosophila
Advanced Science, EarlyView.Current CRISPR interference (CRISPRi) technology in Drosophila has limited efficiency. This study introduces the emCRISPRi platform, which significantly enhances transcriptional silencing efficacy by embedding inhibitory domains within the dCas9 architecture.
Pengchong Fu +7 more
wiley +1 more source
Asymmetrically Modified Nucleosomes [PDF]
Cell, 2012 Mononucleosomes, the basic building blocks of chromatin, contain two copies of each core histone. The associated posttranslational modifications regulate essential chromatin-dependent processes, yet whether each histone copy is identically modified in vivo is unclear.
Voigt, Philipp +8 more
openaire +2 more sources
Promotion of DFU Wound Healing via BRG1–COL16A1 Axis in Fibroblasts
Advanced Science, EarlyView.In normal wound healing, transcription factor BRG1 is upregulated and binds the COL16A1 promoter to enhance its expression, promoting fibroblast proliferation, migration, contraction, extracellular matrix deposition, and granulation tissue formation, thus accelerating wound closure.
Penghui Wang +10 more
wiley +1 more source
NAP1-assisted nucleosome assembly on DNA measured in real time by single-molecule magnetic tweezers.
PLoS ONE, 2012 While many proteins are involved in the assembly and (re)positioning of nucleosomes, the dynamics of protein-assisted nucleosome formation are not well understood.
Rifka Vlijm +3 more
doaj +1 more source
Diversity of eukaryotic DNA replication origins revealed by genome-wide analysis of chromatin structure. [PDF]
PLoS Genetics, 2010 Eukaryotic DNA replication origins differ both in their efficiency and in the characteristic time during S phase when they become active. The biological basis for these differences remains unknown, but they could be a consequence of chromatin structure ...
Nicolas M Berbenetz +2 more
doaj +1 more source
Advanced Science, EarlyView.Donor‐derived tdTomato+ mature hepatocytes were FACS‐isolated and transplanted into Fah−/− host mice. During regeneration, these cells convert into proliferative, unipotent Afp+ rHeps. Their plasticity is governed by a PPARγ/AFP‐dependent metabolic switch, segregating into pro‐proliferative Afplow and pro‐survival Afphigh subpopulations.
Ting Fang +12 more
wiley +1 more source
Advanced Science, EarlyView.This study reveals a pivotal epigenetic regulatory role for the histone acetyltransferase p300, demonstrating that its acetylation of histone 3 at lysine 18 and 27 (H3K18ac and H3K27ac), alongside the formation of the p300/BRD4/SP1 complex, drives AR activation and ovarian fibrosis in PCOS.
Zhengquan Zhu +11 more
wiley +1 more source
Defining transcription factor nucleosome binding with Pioneer-seq.
PLoS GeneticsGene expression requires the targeting of transcription factors (TFs) to regulatory sequences often occluded within nucleosomes. To comprehensively examine TF nucleosome binding, we developed Pioneer-Seq.
Maria Tsompana +4 more
doaj +1 more source