Results 11 to 20 of about 2,759,679 (292)
siDirect 2.0: updated software for designing functional siRNA with reduced seed-dependent off-target effect [PDF]
BMC Bioinformatics, 2009 Background RNA interference (RNAi), mediated by 21-nucleotide (nt)-length small interfering RNAs (siRNAs), is a powerful tool not only for studying gene function but also for therapeutic applications.
Morishita Shinichi +3 more
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Frontiers in Microbiology, 2021 Viral DNA integrated in host cells is a major barrier to completely curing HIV-1. However, genome editing using the recently developed technique of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has the potential to eradicate HIV-
Yufan Xu +8 more
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Quantifying CRISPR off-target effects [PDF]
Emerging Topics in Life Sciences, 2019 Abstract Recent advances in the era of genetic engineering have significantly improved our ability to make precise changes in the genomes of human cells. Throughout the years, clinical trials based on gene therapies have led to the cure of diseases such as X-linked severe combined immunodeficiency (SCID-X1), adenosine deaminase ...
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CRISPR-Cas9 System for Plant Genome Editing: Current Approaches and Emerging Developments
Agronomy, 2020 Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic engineering tool in various biological systems. This editing technology has been in the limelight due to its simplicity and versatility compared to other previously known ...
Jake Adolf V. Montecillo +2 more
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Journal of Integrative Agriculture, 2022 A recent breakthrough in agricultural biotechnology is the introduction of RNAi-mediated strategies in pest control. However, the off-target effects of RNAi pest control are still not fully understood.
Wei-hua MA +10 more
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Strategies for High-Efficiency Mutation Using the CRISPR/Cas System
Frontiers in Cell and Developmental Biology, 2022 Clustered regularly interspaced short palindromic repeats (CRISPR)-associated systems have revolutionized traditional gene-editing tools and are a significant tool for ameliorating gene defects.
Shuying Feng +10 more
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Off-Target Effects of MEK Inhibitors [PDF]
Biochemistry, 2013 The mitogen-activated protein kinases (MAPKs) ERK1/2 regulate numerous cellular processes, including gene transcription, proliferation, and differentiation. The only known substrates of the MAP2Ks MEK1/2 are ERK1/2; thus, MEK inhibitors PD98059, U0126, and PD0325901 have been important tools in determining the functions of ERK1/2.
Eric M, Wauson +4 more
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Prediction of off-target effects of the CRISPR/Cas9 system for design of sgRNA [PDF]
E3S Web of Conferences, 2020 CRISPR/Cas9 genome editing technology is the frontier of life science research. They have been used to cure human genetic diseases, achieve cell personalized treatment, develop new drugs, and improve the genetic characteristics of crops and other fields.
Guo Calvin, Zhen David
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Molecular Therapy: Nucleic Acids, 2023 Paired SpCas9 nickases (SpCas9n) are an effective strategy to reduce off-target effect in genome editing. However, this approach is not efficient with 3′-overhanging ends, limiting its applications.
Yue Wang +6 more
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Frontiers in Oncology, 2019 Background: Localized radiation therapy (RT) is known to infrequently cause off-target or “abscopal” effects at distant metastatic lesions. The mechanism through which abscopal effects occur remains unknown, but is thought to be caused by a humoral ...
Danielle Golub +4 more
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