Results 21 to 30 of about 2,759,679 (292)
siRNA design software for a target gene-specific RNA interference
Frontiers in Genetics, 2012 RNA interference (RNAi) is the mechanism through which small interfering RNA (siRNA) induces sequence-specific posttranscriptional gene silencing. RNAi is commonly recognized as a powerful tool not only for functional genomics but also for therapeutic ...
Yuki eNaito +3 more
doaj +1 more source
Off- and on-target effects of genome editing in mouse embryos
The Journal of Reproduction and Development, 2018 Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome.
Shinya AYABE +2 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2022 The CRISPR-Cas9 system has emerged as a powerful and efficient tool for genome editing. An important drawback of the CRISPR-Cas9 system is the constitutive endonuclease activity when Cas9 endonuclease and its sgRNA are co-expressed.
Seung Bum Park +8 more
doaj +1 more source
Off-target effects of torcetrapib [PDF]
Nature Reviews Cardiology, 2010 The adverse off-target effects of the cholesteryl ester transfer protein (CETP) inhibitor torcetrapib may be mediated by L-type calcium channels, shows a study published in the Journal of Hypertension.
openaire +2 more sources
Non-contact online thickness measurement system for metal films based on eddy current sensing with distance tracking technique [PDF]
, 2016 This paper proposes an online, non-contact metal film thickness measurement system based on eddy current sensing. The slope of the lift-off curve (LOC) is used for characterizing target thickness.
Feng, Z, Li, W, Wang, H
core +1 more source
Frontiers in Molecular Neuroscience, 2021 Variants of the SH3 and multiple ankyrin repeat domains 3 (SHANK3), which encodes postsynaptic scaffolds, are associated with brain disorders. The targeted alleles in a few Shank3 knock-out (KO) lines contain a neomycin resistance (Neo) cassette, which ...
Chunmei Jin +26 more
doaj +1 more source
Offâtarget effects of engineered nucleases [PDF]
The FEBS Journal, 2016 Recent advances in gene editing with engineered nucleases have transformed our ability to manipulate the genome from diverse organisms for applications ranging from biomedical research to disease treatment. A major complication with these engineered nucleases is the binding of the nuclease to unintended genomic sites that share sequence homology with ...
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The off-target effects of AID in carcinogenesis
Frontiers in Immunology, 2023 Activation-induced cytidine deaminase (AID) plays a crucial role in promoting B cell diversification through somatic hypermutation (SHM) and class switch recombination (CSR). While AID is primarily associated with the physiological function of humoral immune response, it has also been linked to the initiation and progression of lymphomas. Abnormalities
Junna Jiao +6 more
openaire +3 more sources
Thermodynamic control of small RNA-mediated gene silencing
Frontiers in Genetics, 2012 Small interfering RNAs (siRNAs) and microRNAs (miRNAs) are crucial regulators of posttranscriptional gene silencing, which is referred to as RNA interference (RNAi) or RNA silencing.
Kumiko eUi-Tei +3 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2022 Genome editing in pluripotent stem cells (PSCs) using CRISPR technology holds great promise for therapeutic applications. Yet, it has been reported that Cas9-mediated cleavage could cause large deletions or rearrangements of DNA, and the selection of ...
Zhenwu Zhang +7 more
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