Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus [PDF]
Current Issues in Molecular Biology, 2023 Anti-DNA antibodies are known to be classical serological hallmarks of systemic lupus erythematosus (SLE). In addition to high-affinity antibodies, the autoantibody pool also contains natural catalytic anti-DNA antibodies that recognize and hydrolyze DNA.
Tatiana S. Novikova +6 more
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Experimental optimization of probe length to increase the sequence specificity of high-density oligonucleotide microarrays [PDF]
BMC Genomics, 2007 Background High-density oligonucleotide arrays are widely used for analysis of genome-wide expression and genetic variation. Affymetrix GeneChips – common high-density oligonucleotide arrays – contain perfect match (PM) and mismatch (MM) probes generated
Kashiwagi Akiko +4 more
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Efficiency of genome editing using modified single-stranded oligodeoxyribonucleotides in human cells [PDF]
Scientific ReportsSingle-stranded oligodeoxyribonucleotide (ssODN) gene editing has emerged as a promising therapeutic strategy. However, further improvements in efficiency are desired for practical application.
Seryoung Kim +3 more
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Molecules, 2023 Raman nanoparticle probes are a potent class of optical labels for the interrogation of pathological and physiological processes in cells, bioassays, and tissues.
Monica-Cornelia Sardaru +4 more
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Biomolecules, 2020 Human neutrophils are the first line of defense against bacterial and viral infections. They eliminate pathogens through phagocytosis, which activate the 5-lipoxygenase (5-LOX) pathway resulting in synthesis of leukotrienes.
Ekaterina A. Golenkina +6 more
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Facile enzymatic synthesis of base J-containing oligodeoxyribonucleotides and an analysis of the impact of base J on DNA replication in cells. [PDF]
PLoS ONE, 2014 We reported here the use of T4 bacteriophage β-glucosyltransferase (T4 β-GT) for the facile synthesis of base J-containing oligodeoxyribonucleotides (ODNs). We found that the enzyme could catalyze the glucosylation of 5-hydroxymethyl-2-deoxyuridine (5hmU)
Debin Ji, Yinsheng Wang
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DNA (Cytosine-C5) methyltransferase inhibition by oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone (zebularine aglycon) at the enzymatic target site [PDF]
, 2009 20 pages, 7 figures, 1 table.-- PMID: 19467223 [PubMed].-- PMCID: PMC2756644.-- NIHMSID: NIHMS130041.-- Printed version published Sep 15, 2009.Aberrant cytosine methylation in promoter regions leads to gene silencing associated with cancer progression. A
Van Bemmel, Dana M. +4 more
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A Solution to the Common Problem of the Synthesis and Applications of Hexachlorofluorescein Labeled Oligonucleotides. [PDF]
PLoS ONE, 2016 A common problem of the preparation of hexachlorofluorescein labeled oligonucleotides is the transformation of the fluorophore to an arylacridine derivative under standard ammonolysis conditions. We show here that the arylacridine byproduct with distinct
Andrey N Chuvilin +4 more
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Pathogens, 2021 Mimicking bacterial DNA, synthetic CpG-containing oligodeoxyribonucleotides (CpG-ODNs) have a powerful immunomodulatory potential. Their practical application is mainly associated with the production of vaccines, where they are used as adjuvants, as well
Ekaterina A. Golenkina +5 more
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Rainbow™ Universal CPG: A Versatile Solid Support for Oligonucleotide Synthesis
BioTechniques, 1997 We have developed a universal solid support, termed Rainbow™ Universal CPG, for use in automated oligonucleotide synthesis. The universal solid support allows any oligodeoxyribonucleotide sequence to be synthesized from a single type of controlled pore ...
P.S. Nelson +4 more
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