Results 21 to 30 of about 11,991 (190)

Sequence requirements for oligodeoxyribonucleotide inhibitory activity [PDF]

International Immunology, 2005
Inhibitory oligonucleotides (IN-ODN) differing from stimulatory CpG ODN (ST-ODN) by as few as two bases can block ST-ODN-induced proliferation, apoptosis protection and IL-6 secretion in B lymphocytes and the production of IL-12p40 by non-B cells. The main objective of this study was to determine the ODN sequence requirements for inhibition in mice ...
Robert F, Ashman, J Adam, Goeken, Jennifer, Drahos, Petar, Lenert   +3 more
openaire   +2 more sources

Transport Oligonucleotides—A Novel System for Intracellular Delivery of Antisense Therapeutics

Molecules, 2020
Biological activity of antisense oligonucleotides (asON), especially those with a neutral backbone, is often attenuated by poor cellular accumulation. In the present proof-of-concept study, we propose a novel delivery system for asONs which implies the ...
Oleg V. Markov, Anton V. Filatov, Maxim S. Kupryushkin, Ivan V. Chernikov, Olga A. Patutina, Anton A. Strunov, Elena L. Chernolovskaya, Valentin V. Vlassov, Dmitrii V. Pyshnyi, Marina A. Zenkova   +9 more
doaj   +1 more source

Preparation of a disulfide-linked precipitative soluble support for solution-phase synthesis of trimeric oligodeoxyribonucleotide 3´-(2-chlorophenylphosphate) building blocks

Beilstein Journal of Organic Chemistry, 2015
The preparation of a disulfide-tethered precipitative soluble support and its use for solution-phase synthesis of trimeric oligodeoxyribonucleotide 3´-(2-chlorophenylphosphate) building blocks is described. To obtain the building blocks, N-acyl protected
Amit M. Jabgunde, Alejandro Gimenez Molina, Pasi Virta, Harri Lönnberg   +3 more
doaj   +1 more source

Non-Nucleosidic Analogues of Polyaminonucleosides and Their Influence on Thermodynamic Properties of Derived Oligonucleotides

Molecules, 2015
The rationale for the synthesis of cationic modified nucleosides is higher expected nuclease resistance and potentially better cellular uptake due to an overall reduced negative charge based on internal charge compensation.
Jolanta Brzezinska, Wojciech T. Markiewicz   +1 more
doaj   +1 more source

Extensive trimming of short single-stranded DNA oligonucleotides during replication-coupled gene editing in mammalian cells.

PLoS Genetics, 2020
Through transfection of short single-stranded oligodeoxyribonucleotides (ssODNs) small genomic alterations can be introduced into mammalian cells with high precision. ssODNs integrate into the genome during DNA replication, but the resulting heteroduplex
Thomas W van Ravesteyn, Marcos Arranz Dols, Wietske Pieters, Marleen Dekker, Hein Te Riele   +4 more
doaj   +1 more source

Anomalous hairpin formation in an oligodeoxyribonucleotide [PDF]

Nucleic Acids Research, 1985
An accurate method for deriving molar absorptivity-temperature profiles applied to a set of single-stranded oligodeoxyribonucleotides shows that the undecamer CGAGTTTGACGp exists in a hairpin conformation involving Watson-Crick base pairing between the two terminal CG dinucleotides.
J G, Nadeau, P T, Gilham
openaire   +2 more sources

An update on targeted gene repair in mammalian cells: methods and mechanisms

Journal of Biomedical Science, 2011
Transfer of full-length genes including regulatory elements has been the preferred gene therapy strategy for clinical applications. However, with significant drawbacks emerging, targeted gene alteration (TGA) has recently become a promising alternative ...
Bolund Lars, Sørensen Charlotte B, Nielsen Roni R, Jakobsen Maria, Dalsgaard Trine, Jensen Nanna M, Jensen Thomas G   +6 more
doaj   +1 more source

Oligonucleotides blocking glucosylceramide synthase expression selectively reverse drug resistance in cancer cells

Journal of Lipid Research, 2004
High glucosylceramide synthase (GCS) activity is one factor contributing to multidrug resistance (MDR) in breast cancer. Enforced GCS overexpression has been shown to disrupt ceramide-induced apoptosis and to confer resistance to doxorubicin.
Yong-Yu Liu, Tie Yan Han, Jing Yuan Yu, Arie Bitterman, Ahn Le, Armando E. Giuliano, Myles C. Cabot   +6 more
doaj   +1 more source

Experimental optimization of probe length to increase the sequence specificity of high-density oligonucleotide microarrays

BMC Genomics, 2007
Background High-density oligonucleotide arrays are widely used for analysis of genome-wide expression and genetic variation. Affymetrix GeneChips – common high-density oligonucleotide arrays – contain perfect match (PM) and mismatch (MM) probes generated
Kashiwagi Akiko, Furusawa Chikara, Ono Naoaki, Suzuki Shingo, Yomo Tetsuya   +4 more
doaj   +1 more source

Antibodies elicited by defined oligodeoxyribonucleotide sequences

Nucleic Acids Research, 1978
Antibodies to the oligodeoxyribonucleotides d(pT)3, d(pT)4, d(pT)6 and d(pA-A-T-T) were elicited in rabbits by immunization with electrostatic complexes of the respective haptens with methylated bovine serum albumin (MBSA). The antisera were assayed by complement fixation using denatured DNA's of various sources as antigens.
H J, Störl, H, Simon, H, Barthelmes
openaire   +3 more sources