Results 31 to 40 of about 15,454 (234)

Determination of Methylation Site of DNA-Methyltransferase NlaX by a Hybrid Method

BioTechniques, 2002
Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M·NlaX) from Neisseria lactamica was established to be
E.A. Kubareva, J. Walter, A.S. Karyagina, O.V. Vorob'eva, P.C.K. Lau, T. Trautner   +5 more
doaj   +1 more source

Synthesis and anti-HIV activity of thiocholesteryl-coupled phosphodiester antisense oligonucleotides incorporated into immunoliposomes [PDF]

, 1994
Encapsulation of oligonucleotides in antibody-targeted liposomes (immunoliposomes) which bind to target cells permits intracellular delivery of the oligonucleotides.
Akhtar, Akhtar, Barré-Sinoussi, Boutorine, Boutorine, Boutorine, Boutorine, de Smidt, Feener, Gamper, Goodchild, Hope, Kabanov, Kim, Kinchington, Krieg, Lemke, Leonetti, Leserman, Leserman, Letsinger, Loke, Machy, Machy, Machy, Matsukura, Matthay, Oberhauser, Rebaï, Rey, Saison-Behmoaras, Senior, Shaw, Shea, Stein, Stewart, Suzuki, Svinarchuk, Thierry, Uhlmann, Yakubov, Zamecnik, Zelphati   +42 more
core   +1 more source

Microwave-assisted rapid deprotection of oligodeoxyribonucleotides [PDF]

Nucleic Acids Research, 1997
A novel method for the deprotection of oligodeoxyribonucleotides under microwave irradiation has been developed. The oligodeoxynucleotides having base labile, phenoxyacetyl (pac), protection for exocyclic amino functions were fully deprotected in 0. 2 M sodium hydroxide (methanol:water : : 1:1, v/v) = A and 1 M sodium hydroxide (methanol:water : : 1:1,
P. Kumar, Kailash C. Gupta
openaire   +3 more sources

Preparation of a disulfide-linked precipitative soluble support for solution-phase synthesis of trimeric oligodeoxyribonucleotide 3´-(2-chlorophenylphosphate) building blocks

Beilstein Journal of Organic Chemistry, 2015
The preparation of a disulfide-tethered precipitative soluble support and its use for solution-phase synthesis of trimeric oligodeoxyribonucleotide 3´-(2-chlorophenylphosphate) building blocks is described. To obtain the building blocks, N-acyl protected
Amit M. Jabgunde, Alejandro Gimenez Molina, Pasi Virta, Harri Lönnberg   +3 more
doaj   +1 more source

Non-Nucleosidic Analogues of Polyaminonucleosides and Their Influence on Thermodynamic Properties of Derived Oligonucleotides

Molecules, 2015
The rationale for the synthesis of cationic modified nucleosides is higher expected nuclease resistance and potentially better cellular uptake due to an overall reduced negative charge based on internal charge compensation.
Jolanta Brzezinska, Wojciech T. Markiewicz   +1 more
doaj   +1 more source

Transport Oligonucleotides—A Novel System for Intracellular Delivery of Antisense Therapeutics

Molecules, 2020
Biological activity of antisense oligonucleotides (asON), especially those with a neutral backbone, is often attenuated by poor cellular accumulation. In the present proof-of-concept study, we propose a novel delivery system for asONs which implies the ...
Oleg V. Markov, Anton V. Filatov, Maxim S. Kupryushkin, Ivan V. Chernikov, Olga A. Patutina, Anton A. Strunov, Elena L. Chernolovskaya, Valentin V. Vlassov, Dmitrii V. Pyshnyi, Marina A. Zenkova   +9 more
doaj   +1 more source

Imidazopurinones are markers of physiological genomic damage linked to DNA instability and glyoxalase 1-associated tumour multidrug resistance [PDF]

, 2010
Glyoxal and methylglyoxal are reactive dicarbonyl metabolites formed and metabolized in physiological systems. Increased exposure to these dicarbonyls is linked to mutagenesis and cytotoxicity and enhanced dicarbonyl metabolism by overexpression of ...
Baynes, Boyum, Brigitte M. Winklhofer-Roob, Cao, Cao, Chan, Chung, Cooke, Cooke, De Bont, Dobler, Duewelhenke, Fleming, Frischmann, Gatenby, Hou, Kang, Krymkiewicz, Larisch, Li, Luo, Michael R. Stratton, Morcos, Naila Rabbani, Nicolay, Papoulis, Paul J. Thornalley, Pfaffl, Pischetsrieder, Rock, Sahar Waris, Sakamoto, Sakamoto, Sarah J. Larkin, Schneider, Seidel, Shapiro, Synold, Thomas Fleming, Thomas Santarius, Thornalley, Thornalley, Thornalley, Thornalley, Thornalley, Vaca, Wang, Yeluri, Yuan   +48 more
core   +2 more sources

A novel approach to the synthesis of oligodeoxyribonucleotide boranophosphates [PDF]

Nucleic Acids Symposium Series, 2000
Deoxyribonucleoside 3'-boranophosphate derivatives including adenine, cytosine, guanine, and thymine bases were synthesized in good yields by the use of a new boranophosphorylation reaction. The reaction was found to be effective for the formation of internucleotidic boranophosphate linkages.
Mamoru Shimizu, Natsuhisa Oka, Kazuhiko Saigo, Takeshi Wada   +3 more
openaire   +3 more sources

Extensive trimming of short single-stranded DNA oligonucleotides during replication-coupled gene editing in mammalian cells.

PLoS Genetics, 2020
Through transfection of short single-stranded oligodeoxyribonucleotides (ssODNs) small genomic alterations can be introduced into mammalian cells with high precision. ssODNs integrate into the genome during DNA replication, but the resulting heteroduplex
Thomas W van Ravesteyn, Marcos Arranz Dols, Wietske Pieters, Marleen Dekker, Hein Te Riele   +4 more
doaj   +1 more source

Biophysical and biological properties of quadruplex oligodeoxyribonucleotides [PDF]

Nucleic Acids Research, 2003
Single-stranded guanosine-rich oligodeoxyribonucleotides (GROs) have a propensity to form quadruplex structures that are stabilized by G-quartets. In addition to intense speculation about the role of G-quartet formation in vivo, there is considerable interest in the therapeutic potential of quadruplex oligonucleotides as aptamers or non-antisense ...
Vedra Abdomerović, Paula J. Bates, John O. Trent, Virna Đapić, Jemma C. Peberdy, Rachel Marrington, Alison Rodger   +6 more
openaire   +3 more sources