The cDNA and deduced amino acid sequence of the γ subunit of the L-type calcium channel from rabbit skeletal muscle [PDF]
, 1990 Complementary DNAs for the γ subunit of the calcium channel of rabbit skeletal muscle were isolated on the basis of peptide sequences derived from the purified protein.
Biel +30 more
core +1 more source
An update on targeted gene repair in mammalian cells: methods and mechanisms
Journal of Biomedical Science, 2011 Transfer of full-length genes including regulatory elements has been the preferred gene therapy strategy for clinical applications. However, with significant drawbacks emerging, targeted gene alteration (TGA) has recently become a promising alternative ...
Bolund Lars +6 more
doaj +1 more source
Identifying the impact of G-quadruplexes on Affymetrix 3' arrays using cloud computing. [PDF]
, 2010 A tetramer quadruplex structure is formed by four parallel strands of DNA/ RNA containing runs of guanine. These quadruplexes are able to form because guanine can Hoogsteen hydrogen bond to other guanines, and a tetrad of guanines can form a stable ...
Harrison, AP +4 more
core +2 more sources
Reversible DNA micro-patterning using the fluorous effect [PDF]
, 2017 We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of ...
Burley, Glenn A. +7 more
core +1 more source
Sequence requirements for oligodeoxyribonucleotide inhibitory activity [PDF]
International Immunology, 2005 Inhibitory oligonucleotides (IN-ODN) differing from stimulatory CpG ODN (ST-ODN) by as few as two bases can block ST-ODN-induced proliferation, apoptosis protection and IL-6 secretion in B lymphocytes and the production of IL-12p40 by non-B cells. The main objective of this study was to determine the ODN sequence requirements for inhibition in mice ...
Petar Lenert +3 more
openaire +3 more sources
Two-dimensional protein crystallization via metal-ion coordination by naturally occurring surface histidines [PDF]
, 1996 A powerful and potentially general approach to the targeting and crystallization of proteins on lipid interfaces through coordination of surface histidine residues to lipid-chelated divalent metal ions is presented.
Arnold, Frances H. +7 more
core +1 more source
, 2005 Toll-like receptor (TLR)-9 recognizes CpG motifs in microbial DNA. TLR9 signalling stimulates innate antimicrobial immunity and modulates adaptive immune responses including autoimmunity against chromatin, e.g., in systemic lupus erythematosus (SLE ...
Anders HJ, H-J Anders, Stacey KJ
core +1 more source
New findings on the d(TGGGAG) sequence: Surprising anti-HIV-1 activity [PDF]
, 2018 The biological relevance of tetramolecular G-quadruplexes especially as anti-HIV agents has been extensively reported in the literature over the last years. In the light of our recent results regarding the slow G-quadruplex folding kinetics of ODNs based
Di Fabio, Giovanni +5 more
core +1 more source
Vortex fluidics-mediated DNA rescue from formalin-fixed museum specimens. [PDF]
, 2020 DNA from formalin-preserved tissue could unlock a vast repository of genetic information stored in museums worldwide. However, formaldehyde crosslinks proteins and DNA, and prevents ready amplification and DNA sequencing.
Girguis, Peter R +6 more
core +1 more source
Immobilization of oligodeoxyribonucleotides with multiple anchors to microchips [PDF]
Nucleic Acids Research, 2001 Facile modification of oligodeoxyribonucleotides is required for efficient immobilization to a pre-activated glass surface. This report presents an oligodeoxyribonucleotide which contains a hairpin stem-loop structure with multiple phosphorothioate moieties in the loop.
Satyam Nampalli +3 more
openaire +3 more sources