The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans [PDF]
Biology, 2023 Understanding human DNA replication through the study of yeast has been an extremely fruitful journey. The minichromosome maintenance (MCM) 2–7 genes that encode the catalytic core of the eukaryotic replisome were initially identified through forward ...
Bik-Kwoon Tye, Yuanliang Zhai
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Origin recognition complex subunit 1(ORC1) is a potential biomarker and therapeutic target in cancer [PDF]
BMC Medical Genomics, 2023 Background The origin recognition complex 1 (ORC1) is a large subunit of the origin recognition complex and acts as the master subunit of the precoding complex. Objective To explore potential function and clinical significance of ORC1 in cancers. Methods
Linling Wu, Hui Chen, Chao Yang
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Developmental regulation of the Tetrahymena thermophila origin recognition complex. [PDF]
PLoS Genetics, 2015 The Tetrahymena thermophila DNA replication machinery faces unique demands due to the compartmentalization of two functionally distinct nuclei within a single cytoplasm, and complex developmental program.
Po-Hsuen Lee +2 more
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Elevated origin recognition complex subunit 6 expression promotes non-small cell lung cancer cell growth [PDF]
Cell Death and DiseaseExploring novel targets for non-small cell lung cancer (NSCLC) remains of utmost importance. This study focused on ORC6 (origin recognition complex subunit 6), investigating its expression and functional significance within NSCLC.
Yong-hua Sang +6 more
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Humanizing the yeast origin recognition complex [PDF]
Nature Communications, 2021 In most model yeast species the Origin Recognition Complex (ORC) binds defined and species-specific base sequences while in humans what determines the binding appears to be more complex.
Clare S. K. Lee +9 more
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Depletion of the Origin Recognition Complex Subunits Delays Aging in Budding Yeast [PDF]
Cells, 2022 Precise DNA replication is pivotal for ensuring the accurate inheritance of genetic information. To avoid genetic instability, each DNA fragment needs to be amplified only once per cell cycle.
Karolina Stępień +4 more
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The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure [PDF]
eLife, 2021 The origin recognition complex (ORC) cooperates with CDC6, MCM2-7, and CDT1 to form pre-RC complexes at origins of DNA replication. Here, using tiling-sgRNA CRISPR screens, we report that each subunit of ORC and CDC6 is essential in human cells. Using an
Hsiang-Chen Chou +12 more
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The dynamic nature of the human origin recognition complex revealed through five cryoEM structures [PDF]
eLife, 2020 Genome replication is initiated from specific origin sites established by dynamic events. The Origin Recognition Complex (ORC) is necessary for orchestrating the initiation process by binding to origin DNA, recruiting CDC6, and assembling the MCM ...
Matt J Jaremko +4 more
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Origin recognition complex 6 overexpression promotes growth of glioma cells [PDF]
Cell Death and DiseaseThe discovery of novel oncotargets for glioma is of immense significance. We here explored the expression patterns, biological functions, and underlying mechanisms associated with ORC6 (origin recognition complex 6) in glioma.
Wen-lei Yang +5 more
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Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6 [PDF]
Nature Communications, 2020 The origin recognition complex (ORC) is essential for loading the Mcm2–7 replicative helicase onto DNA during DNA replication initiation. Here, the authors describe several cryo-electron microscopy structures of Drosophila ORC bound to DNA and its ...
Jan Marten Schmidt, Franziska Bleichert
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