Analysis of cilia dysfunction phenotypes in zebrafish embryos depleted of Origin recognition complex factors. [PDF]
Eur J Hum Genet, 2019 Meier–Gorlin syndrome (MGS) is a rare, congenital primordial microcephalic dwarfism disorder. MGS is caused by genetic variants of components of the origin recognition complex (ORC) consisting of ORC1–6 and the pre-replication complex, which together ...
Maerz LD +5 more
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Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation. [PDF]
Nucleic Acids Res, 2016 Initiation of DNA replication depends upon recognition of genomic sites, termed origins, by AAA+ ATPases. In prokaryotes a single factor binds each origin, whereas in eukaryotes this role is played by a six-protein origin recognition complex (ORC).
Marques CA +7 more
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The remarkable gymnastics of ORC
eLife, 2022 As a cell prepares to divide, a molecular actor known as the Origin Recognition Complex makes intricate ATP-driven movements to recruit proteins required to duplicate DNA.
Bruce Stillman
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Nature Communications, 2023 Chromatin replication involves the assembly and activity of the replisome within the nucleosomal landscape. At the core of the replisome is the Mcm2-7 complex (MCM), which is loaded onto DNA after binding to the Origin Recognition Complex (ORC). In yeast,
Humberto Sánchez +5 more
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Reinventing heterochromatin in budding yeasts: Sir2 and the origin recognition complex take center stage. [PDF]
Eukaryot Cell, 2011 Hickman MA, Froyd CA, Rusche LN.
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Identification of a Novel 81-kDa Component of the Xenopus Origin Recognition Complex [PDF]
, 1998 The Xenopus origin recognition complex is essential for chromosomal DNA replication in cell-free extracts. We have immunopurified the Xenopus origin recognition complex with anti-Xorc2 antibodies and analyzed its composition and properties.
Carpenter, Phillip B. +1 more
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Nature Communications, 2023 The dynamics of DNA replication factors is crucial for cell cycle progression. Arabidopsis encode two origin recognition complex 1 proteins, ORC1a and ORC1b, with distinct roles in DNA replication and deposition of heterochromatic H3K27me1, respectively.
Zaida Vergara +10 more
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In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization [PDF]
, 2013 The origin recognition complex (ORC) of Saccharomyces cerevisiae binds origin DNA and cooperates with Cdc6 and Cdt1 to load the replicative helicase MCM2–7 onto DNA. Helicase loading involves two MCM2–7 hexamers that assemble into a double hexamer around
A. Fernandez-Cid +47 more
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Nucleosome-directed replication origin licensing independent of a consensus DNA sequence
Nature Communications, 2022 Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions
Sai Li +5 more
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ATP dependent assembly of the human origin recognition complex [PDF]
, 2007 The Origin Recognition Complex (ORC) was initially discovered in budding yeast extracts as a protein complex that binds with high affinity to Autonomously Replicating Sequences (ARS) in an ATP dependent manner.
Siddiqui, K., Stillman, B.
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