Results 21 to 30 of about 196,060 (141)

The origin recognition complex: a biochemical and structural view. [PDF]

Subcell Biochem, 2012
The origin recognition complex (ORC) was first discovered in the baker's yeast in 1992. Identification of ORC opened up a path for subsequent molecular level investigations on how eukaryotic cells initiate and control genome duplication each cell cycle.
Li H, Stillman B.
europepmc   +4 more sources

Origin recognition complex subunit 6 (ORC6) is a key mediator of LPS-induced NFκB activation and the pro-inflammatory response [PDF]

Cell Communication and Signaling
Lipopolysaccharide (LPS)-activated pro-inflammatory responses play a critical role in sepsis, a life-threatening condition. This study investigates the role of origin recognition complex subunit 6 (ORC6) in LPS responses in macrophages and monocytes ...
Zichen Xie, Haisu Lu, Jiayi Zheng, Jianfeng Song, Keyu Sun   +4 more
doaj   +2 more sources

Phosphorylation of ORC2 protein dissociates origin recognition complex from chromatin and replication origins. [PDF]

J Biol Chem, 2012
During the late M to the G(1) phase of the cell cycle, the origin recognition complex (ORC) binds to the replication origin, leading to the assembly of the prereplicative complex for subsequent initiation of eukaryotic chromosome replication. We found that the cell cycle-dependent phosphorylation of human ORC2, one of the six subunits of ORC ...
Lee KY, Bang SW, Yoon SW, Lee SH, Yoon JB, Hwang DS.   +5 more
europepmc   +4 more sources

The metazoan origin recognition complex [PDF]

Frontiers in Bioscience, 1999
Regulated initiation of DNA replication relies on the firing of initiator proteins that bind specifically to origin DNA. The discovery of the first eukaryotic initiator, the Saccharomyces cerevisiae Origin Recognition Complex (ORC) has allowed us to discern some aspects of how the onset of replication is regulated.
D G, Quintana, A, Dutta
openaire   +2 more sources

Origin recognition complex binding to a metazoan replication origin [PDF]

Current Biology, 2001
The initiation of DNA replication in eukaryotic cells at the onset of S phase requires the origin recognition complex (ORC) [1]. This six-subunit complex, first isolated in Saccharomyces cerevisiae [2], is evolutionarily conserved [1]. ORC participates in the formation of the prereplicative complex [3], which is necessary to establish replication ...
Bielinsky, Anja-Katrin, Blitzblau, Hannah, Beall, Eileen L., Ezrokhi, Michael, Smith, Heidi S., Botchan, Michael R., Gerbi, Susan A.   +6 more
openaire   +2 more sources

A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint

Nature Communications, 2023
Chromatin replication involves the assembly and activity of the replisome within the nucleosomal landscape. At the core of the replisome is the Mcm2-7 complex (MCM), which is loaded onto DNA after binding to the Origin Recognition Complex (ORC). In yeast,
Humberto Sánchez, Zhaowei Liu, Edo van Veen, Theo van Laar, John F. X. Diffley, Nynke H. Dekker   +5 more
doaj   +1 more source

The remarkable gymnastics of ORC

eLife, 2022
As a cell prepares to divide, a molecular actor known as the Origin Recognition Complex makes intricate ATP-driven movements to recruit proteins required to duplicate DNA.
Bruce Stillman
doaj   +1 more source

Architecture of the Human Origin Recognition Complex [PDF]

Journal of Biological Chemistry, 2001
All the human homologs of the six subunits of Saccharomyces cerevisiae origin recognition complex have been reported so far. However, not much has been reported on the nature and the characteristics of the human origin recognition complex. In an attempt to purify recombinant human ORC from insect cells infected with baculoviruses expressing HsORC ...
S K, Dhar, L, Delmolino, A, Dutta
openaire   +2 more sources

Distinct roles of Arabidopsis ORC1 proteins in DNA replication and heterochromatic H3K27me1 deposition

Nature Communications, 2023
The dynamics of DNA replication factors is crucial for cell cycle progression. Arabidopsis encode two origin recognition complex 1 proteins, ORC1a and ORC1b, with distinct roles in DNA replication and deposition of heterochromatic H3K27me1, respectively.
Zaida Vergara, María S. Gomez, Bénédicte Desvoyes, Joana Sequeira-Mendes, Kinda Masoud, Celina Costas, Sandra Noir, Elena Caro, Victoria Mora-Gil, Pascal Genschik, Crisanto Gutierrez   +10 more
doaj   +1 more source

Nucleosome-directed replication origin licensing independent of a consensus DNA sequence

Nature Communications, 2022
Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions
Sai Li, Michael R. Wasserman, Olga Yurieva, Lu Bai, Michael E. O’Donnell, Shixin Liu   +5 more
doaj   +1 more source