Results 51 to 60 of about 1,052,584 (308)

In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization [PDF]

, 2013
The origin recognition complex (ORC) of Saccharomyces cerevisiae binds origin DNA and cooperates with Cdc6 and Cdt1 to load the replicative helicase MCM2–7 onto DNA. Helicase loading involves two MCM2–7 hexamers that assemble into a double hexamer around
A. Fernandez-Cid, A. Riera, Bell, Bowers, Bray, C. Evrin, C. Speck, Casas-Terradellas, Chen, Cocker, Coleman, Costa, Donovan, Dueber, Gillespie, Herbig, Holm, J. Zech, Klemm, Krupinski, Laemmli, Liang, Liang, Liu, M. C. Herrera, Mizushima, Neuwald, P. Clarke, Perkins, R. Lurz, Randell, Remus, Remus, Roy, S. Brill, Schepers, Seki, Singleton, Steven, Stoeber, Takara, Tanaka, Watanabe, Weinreich, Wendler, Williams, Ying, Zhang   +47 more
core   +1 more source

Nucleosome-directed replication origin licensing independent of a consensus DNA sequence

Nature Communications, 2022
Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions
Sai Li, Michael R. Wasserman, Olga Yurieva, Lu Bai, Michael E. O’Donnell, Shixin Liu   +5 more
doaj   +1 more source

Assembly of the Human Origin Recognition Complex [PDF]

Journal of Biological Chemistry, 2001
The six-subunit origin recognition complex (ORC) was originally identified in the yeast Saccharomyces cerevisiae. Yeast ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors, such as Cdc6 and the Mcm proteins.
S, Vashee, P, Simancek, M D, Challberg, T J, Kelly   +3 more
openaire   +2 more sources

The origin recognition complex: from simple origins to complex functions [PDF]

Genes & Development, 2002
Eukaryotic DNA replication is an intricate process that requires the coordinate and tightly regulated action of numerous molecular machines. Eukaryotic cells must choose hundreds if not thousands of origins of replication and assemble multiple proteins or protein complexes at these sites. During S phase, these complexes are activated in a reproducible,
openaire   +2 more sources

The origin recognition complex: a biochemical and structural view. [PDF]

Subcell Biochem, 2012
The origin recognition complex (ORC) was first discovered in the baker's yeast in 1992. Identification of ORC opened up a path for subsequent molecular level investigations on how eukaryotic cells initiate and control genome duplication each cell cycle.
Li H, Stillman B.
europepmc   +4 more sources

RNA‐dependent recruitment of the origin recognition complex [PDF]

The EMBO Journal, 2008
The origin recognition complex (ORC) has an important function in determining the initiation sites of DNA replication. In higher eukaryotes, ORC lacks sequence-specific DNA binding, and the mechanisms of ORC recruitment and origin determination are poorly understood.
Julie, Norseen, Andreas, Thomae, Venkatesh, Sridharan, Ashok, Aiyar, Aloys, Schepers, Paul M, Lieberman   +5 more
openaire   +2 more sources

The origin recognition complex is dispensable for endoreplication in Drosophila [PDF]

Proceedings of the National Academy of Sciences, 2008
The origin recognition complex (ORC) is an essential component of the prereplication complex (pre-RC) in mitotic cell cycles. The role of ORC as a foundation to assemble the pre-RC is conserved from yeast to human. Furthermore, in metazoans ORC plays a key role in determining the timing of replication initiation and origin usage ...
So Young Park, Maki Asano
openalex   +3 more sources

The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin

eLife, 2015
Heterochromatic domains are enriched with repressive histone marks, including histone H3 lysine 9 methylation, written by lysine methyltransferases (KMTs).
Sumanprava Giri, Vasudha Aggarwal, Julien Pontis, Zhen Shen, Arindam Chakraborty, Abid Khan, Craig Mizzen, Kannanganattu V Prasanth, Slimane Ait-Si-Ali, Taekjip Ha, Supriya G Prasanth   +10 more
doaj   +1 more source

DNA replication origins retain mobile licensing proteins

Nature Communications, 2021
Eukaryotic DNA replication is regulated to ensure copying of the genome (only) once per cell cycle. Here the authors, using optical trapping and confocal microscopy, demonstrate the dynamics of the origin recognition complex and subsequent intermediates ...
Humberto Sánchez, Kaley McCluskey, Theo van Laar, Edo van Veen, Filip M. Asscher, Belén Solano, John F. X. Diffley, Nynke H. Dekker   +7 more
doaj   +1 more source

A quantitative model of the initiation of DNA replication in Saccharomyces cerevisiae predicts the effects of system perturbations. [PDF]

, 2012
BackgroundEukaryotic cell proliferation involves DNA replication, a tightly regulated process mediated by a multitude of protein factors. In budding yeast, the initiation of replication is facilitated by the heterohexameric origin recognition complex ...
DaSilva, Lance F, Duncker, Bernard P, Gidvani, Rohan D, Ingalls, Brian P, Li, Grace, McConkey, Brendan J, Sudmant, Peter   +6 more
core   +3 more sources