The extended (p)ppGpp family: New dimensions in Stress response [PDF]
Current Research in Microbial Sciences, 2021 Second messenger (p)ppGpp mediated stress response plays a crucial role in bacterial persistence and multiple drug resistance. In E. coli, (p)ppGpp binds to RNA polymerase and upregulates the transcription of genes essential for stress response while ...
Kirtimaan Syal +2 more
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Role of (p)ppGpp in Biofilm Formation by Enterococcus faecalis [PDF]
Applied and Environmental Microbiology, 2012 ABSTRACT Enterococcus faecalis strain OG1RF and its (p)ppGpp-deficient Δ relA , Δ relQ , and Δ relA Δ relQ mutants were grown in biofilms and evaluated for growth profiles, biofilm morphology, cell viability, and ...
Chavez de Paz, Luis E. +3 more
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Novel (p)ppGpp Binding and Metabolizing Proteins of
mBio, 2018 The alarmone (p)ppGpp plays pivotal roles in basic bacterial stress responses by increasing tolerance of various nutritional limitations and chemical insults, including antibiotics.
Yong Zhang +3 more
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Bacillus subtilis produces (p)ppGpp in response to the bacteriostatic antibiotic chloramphenicol to prevent its potential bactericidal effect [PDF]
mLife, 2022 Antibiotics combat bacteria through their bacteriostatic (by growth inhibition) or bactericidal (by killing bacteria) action. Mechanistically, it has been proposed that bactericidal antibiotics trigger cellular damage, while bacteriostatic antibiotics ...
Jin Yang +3 more
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Selective Fluorescent Chemosensor for the Bacterial Alarmone (p)ppGpp
Journal of the American Chemical Society, 2008 We have developed the first selective fluorescent chemosensor (PyDPA) for (p)ppGpp, a bacterial and plant alarmone. By using pyrene-excimer fluorescence, PyDPA shows very good selectivity for (p)ppGpp from among other nucleotides in water. PyDPA was used for the real-time detection of in vitro ppGpp synthesis by bacterial ribosomal complexes.
Rhee, Hyun-Woo +7 more
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(p)ppGpp and DksA play a crucial role in reducing the efficacy of β-lactam antibiotics by modulating bacterial membrane permeability [PDF]
Microbiology SpectrumThe key signaling molecules in the bacterial stress-sensing pathway, the alarmone (p)ppGpp and the transcription factor DksA, play a crucial role in bacterial survival during nutritional deprivation and exposure to xenobiotics by modulating cellular ...
Meenal Chawla +8 more
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Diversity in E. coli (p)ppGpp Levels and Its Consequences [PDF]
Frontiers in Microbiology, 2020 (p)ppGpp is at the core of global bacterial regulation as it controls growth, the most important aspect of life. It would therefore be expected that at least across a species the intrinsic (basal) levels of (p)ppGpp would be reasonably constant.
Beny Spira, Katia Ospino
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How to quantify magic spots - a brief overview of (p)ppGpp detection and quantitation methods [PDF]
Frontiers in Molecular BiosciencesGuanosine tetra- and penta-phosphates, collectively known as (p)ppGpp, are well-known second messengers of cellular stress responses in bacteria and plants.
Filip Gąsior +2 more
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Regulation of (p)ppGpp hydrolysis by a conserved archetypal regulatory domain [PDF]
Nucleic Acids Research, 2018 Sensory and regulatory domains allow bacteria to adequately respond to environmental changes. The regulatory ACT (Aspartokinase, Chorismate mutase and TyrA) domains are mainly found in metabolic-related proteins as well as in long (p)ppGpp synthetase/hydrolase enzymes.
Séverin Ronneau +5 more
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Integrated control of bacterial growth and stress response by (p)ppGpp in Escherichia coli: A seesaw fashion [PDF]
iScienceSummary: To thrive in nature, bacteria have to reproduce efficiently under favorable conditions and persist during stress. The global strategy that integrates the growth control and stress response remains to be explored.
Manlu Zhu, Haoyan Mu, Xiongfeng Dai
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