Results 71 to 80 of about 7,467 (157)

(p)ppGpp and CodY Promote Enterococcus faecalis Virulence in a Murine Model of Catheter-Associated Urinary Tract Infection

mSphere, 2019
In Firmicutes, the nutrient-sensing regulators (p)ppGpp, the effector molecule of the stringent response, and CodY work in tandem to maintain bacterial fitness during infection. Here, we tested (p)ppGpp and codY mutant strains of Enterococcus faecalis in
C. Colomer-Winter, A. L. Flores-Mireles, S. Kundra, S. J. Hultgren, J. A. Lemos   +4 more
doaj   +1 more source

Small Alarmone Synthetase SasA Expression Leads to Concomitant Accumulation of pGpp, ppApp, and AppppA in Bacillus subtilis

Frontiers in Microbiology, 2020
(p)ppGpp is a highly conserved bacterial alarmone which regulates many aspects of cellular physiology and metabolism. In Gram-positive bacteria such as B.
Danny K. Fung, Jin Yang, David M. Stevenson, Daniel Amador-Noguez, Jue D. Wang   +4 more
doaj   +1 more source

Controlled Delivery and Light‐Induced Release of Magic Spot Nucleotides in Escherichia coli

Angewandte Chemie International Edition, Volume 65, Issue 15, 6 April 2026.
We synthesized photocaged, clickable and isotope‐labeled “magic spot” Nucleotides and delivered them into Escherichia coli comparing different approaches. Light‐controlled release inside living cells enabled tracking of their conversion from pppGpp to ppGpp by capillary electrophoresis mass spectrometry and revealed their impact on the growth rate of a
Christoph Popp, Patrick Moser, Anselm Schwoerbel, Xinwei Liu, Johannes Freitag, Pinku Sarmah, Isabel Prucker, Robert Zscherp, Xuan Wang, Philipp Klahn, Hans‐Georg Koch, Gert Bange, Henning J. Jessen   +12 more
wiley   +1 more source

Effects of (p)ppGpp on the Progression of the Cell Cycle of Caulobacter crescentus [PDF]

Journal of Bacteriology, 2014
ABSTRACT Bacteria must control the progression of their cell cycle in response to nutrient availability. This regulation can be mediated by guanosine tetra- or pentaphosphate [(p)ppGpp], which are synthesized by enzymes of the RelA/SpoT homologue (Rsh) family, particularly under starvation conditions.
Gonzalez Diego, Collier Justine
openaire   +5 more sources

Regulation of (p)ppGpp and Its Homologs on Environmental Adaptation, Survival, and Pathogenicity of Streptococci

Frontiers in Microbiology, 2020
Most streptococci are commensals, pathogens, or opportunistic pathogens for humans and animals. Therefore, it is important for streptococci to adapt to the various challenging environments of the host during the processes of infection or colonization, as
Tengfei Zhang, Tengfei Zhang, Jiawen Zhu, Jiawen Zhu, Jiajia Xu, Huabin Shao, Rui Zhou, Rui Zhou, Rui Zhou   +8 more
doaj   +1 more source

The Use of DeepQSAR Models for the Discovery of Peptides With Enhanced Antimicrobial and Antibiofilm Potential

Molecular Informatics, Volume 45, Issue 4, April 2026.
A DeepQSAR framework integrating a feed‐forward neural network and a recurrent neural network (RNN) was developed to identify antimicrobial peptides with enhanced antibiofilm and antimicrobial activity. The feed‐forward model was trained on in‐house antibiofilm IC50 data to capture quantitative structure–activity relationships, and its learned features
Jiaying You, Hazem Mslati, Evan F. Haney, Noushin Akhoundsadegh, Robert E. W. Hancock, Artem Cherkasov   +5 more
wiley   +1 more source

Recent functional insights into the role of (p)ppGpp in bacterial physiology [PDF]

Nature Reviews Microbiology, 2015
The alarmones guanosine tetraphosphate and guanosine pentaphosphate (collectively referred to as (p)ppGpp) are involved in regulating growth and several different stress responses in bacteria. In recent years, substantial progress has been made in our understanding of the molecular mechanisms of (p)ppGpp metabolism and (p)ppGpp-mediated regulation.
Hauryliuk, Vasili, Atkinson, Gemma C., Murakami, Katsuhiko S., Tenson, Tanel, Gerdes, Kenn   +4 more
openaire   +3 more sources

Studies on the Regulation of (p)ppGpp Metabolism and Its Perturbation Through the Over-Expression of Nudix Hydrolases in Escherichia coli

Frontiers in Microbiology, 2020
Stringent response mediated by modified guanosine nucleotides is conserved across bacteria and is regulated through the Rel/Spo functions. In Escherichia coli, RelA and SpoT proteins synthesize the modified nucleotides ppGpp and pppGpp, together referred
Rajeshree Sanyal, Allada Vimala, Rajendran Harinarayanan   +2 more
doaj   +1 more source

Quinolone tolerance in Escherichia coli due to defects in the adenosine ribonucleotides de novo biosynthesis pathway

mLife, Volume 5, Issue 2, Page 217-228, April 2026.
Abstract Central carbon metabolism is thought to link reactive oxygen species (ROS) with antibiotic‐mediated bacterial death. During enrichment screening of Escherichia coli with the first‐generation quinolone oxolinic acid, unstable antibiotic‐tolerant mutants containing deficiencies in purB were obtained.
Weiwei Zhu, Yuejuan Nong, Jie Su, Jingwen Yang, Lina Ma, Yunxin Xue, Dai Wang, Jianjun Niu, Karl Drlica, Xilin Zhao   +9 more
wiley   +1 more source

Genome‐wide screen reveals a universal role of ATP in ciprofloxacin tolerance among genetically distinct Escherichia coli persisters

mLife, Volume 5, Issue 2, Page 180-198, April 2026.
Abstract Bacterial persisters show tolerance to bactericidal antibiotics and play essential roles in chronic infections; however, the general mechanisms underlying persister formation and antibiotic tolerance remain insufficiently characterized. In this study, the Escherichia coli Keio library was used to identify genes involved in ciprofloxacin ...
Zhenfang Mei, Yawen Cai, Jianfeng Huang, Kedong Zhao, Zuqin Zhang, Dandan Yu, Shiyan Lu, Zeying Lai, Thomas K. Wood, Xinmiao Fu   +9 more
wiley   +1 more source