Results 71 to 80 of about 8,365 (192)
A. Wild type or relA mutant strains grown at 37°C in the MOPS derivative medium with or without arginine hydroxamate (100 μg/ml) were labeled with H332PO4 for 4 to 5 generations.
Etienne Dervyn (730354) +3 more
core +1 more source
Complementary roles of (p)ppGpp and Spx under heat stress.
(A) Heat mediated down-regulation of rrnJp1-lacZ transcription in wild type (BHS220), Δspx (BHS222), (p)ppGpp° (BHS319) and Δspx (p)ppGpp° (BHS766) strains as determined by RT-qPCR. Means and SEM of three independent experiments are shown.
Volkhard Kaever (152216) +14 more
core +1 more source
Metabolism‐Based Biomarkers for Rapid Phenotypic Antibiotic Susceptibility Testing
Metabolic differences emerging after antibiotic exposure provide rapid phenotypic signals of susceptibility and resistance. This review summarizes metabolic biomarkers for rapid phenotypic AST, associated with nutrient uptake, respiratory activity, metabolic reprogramming, and enzymatic function. ABSTRACT The accelerating global crisis of antimicrobial
Sha Yu +7 more
wiley +1 more source
Transcriptional profiling of the primary cell envelope stress response (CESR) of Bacillus subtilis exposed to a range of cell wall‐targeting antimicrobials, combining RNAseq and high‐resolution tiling arrays. We revealed the complexity of the CESR, the contribution of extracytoplasmic function sigma factors (ECFs) and two‐component signal transduction ...
Qian Zhang +12 more
wiley +1 more source
Calcium-activated (p)ppGpp Synthetase in Chloroplasts of Land Plants [PDF]
The genetic system of chloroplasts, including the machinery for transcription, translation, and DNA replication, exhibits substantial similarity to that of eubacteria. Chloroplasts are also thought to possess a system for generating guanosine 5'-triphosphate ((p)ppGpp), which triggers the stringent response in eubacteria, with genes encoding ...
Tozawa, Y. +6 more
openaire +2 more sources
Transcription of photosynthetic genes in the plant chloroplast: lessons from bacteria
SUMMARY The RNA polymerase that transcribes photosynthetic genes in the plant chloroplast is the largest known transcription enzyme across all domains of life, comprising 21 subunits of bacterial and eukaryotic origin. Recent structural analyses revealed that the core polymerase, inherited from the cyanobacterial ancestor of the chloroplast, is encased
Jessica Moon +5 more
wiley +1 more source
Viable but Nonculturable State, a Survival Strategy for Salmonella in Aquatic Environments
Transition of Salmonella from a culturable state to a viable but nonculturable state under various environmental stresses and current methods for detecting viable Salmonella in environmental samples. ABSTRACT In the relentless battle for survival, Salmonella has evolved mechanisms to withstand harsh conditions such as extreme temperatures, fluctuating ...
Sanelisiwe Thinasonke Duze +3 more
wiley +1 more source
Replication of plasmids derived from Shiga toxinconverting bacteriophages in starved Escherichia coli [PDF]
The pathogenicity of Shiga toxin-producing Escherichia coli (STEC) depends on the expression of stx genes that are located on lambdoid prophages. Effective toxin production occurs only after prophage induction, and one may presume that replication of the
Nejman, Bożena +4 more
core
We synthesized photocaged, clickable and isotope‐labeled “magic spot” Nucleotides and delivered them into Escherichia coli comparing different approaches. Light‐controlled release inside living cells enabled tracking of their conversion from pppGpp to ppGpp by capillary electrophoresis mass spectrometry and revealed their impact on the growth rate of a
Christoph Popp +12 more
wiley +1 more source
Bacterial Chromosome Replication and DNA Repair During the Stringent Response
The stringent response regulates bacterial growth rate and is important for cell survival under changing environmental conditions. The effect of the stringent response is pleiotropic, affecting almost all biological processes in the cell including ...
Anurag Kumar Sinha +2 more
doaj +1 more source

