Results 11 to 20 of about 6,961 (161)

Multiple Fluorescence-Based PCR-SSCP Analysis Using Internal Fluorescent Labeling of PCR Products

BioTechniques, 1996
The method to internally label PCR products with multiple colored fluorescent dyes was developed and applied to multiple fluorescence-based PCR single-stranded conformational polymorphism (MF-PCRSSCP) analysis.
H. Iwahana, M. Fujimura, Y. Takahashi, T. Iwabuchi, K. Yoshimoto, M. Itakura   +5 more
doaj   +1 more source

Correlation between large FBN1 deletions and severe cardiovascular phenotype in Marfan syndrome: Analysis of two novel cases and analytical review of the literature

Molecular Genetics &Genomic Medicine, Volume 11, Issue 7, July 2023., 2023
Cardiovascular manifestation is a known phenomenon among Marfan patients. Several genotype–phenotype studies have been performed to find association between cardiovascular disease severity and FBN1 gene mutations. However, these studies focused on intragenic small‐scale mutations, and until now no study was performed to explore the connection between ...
Gergely Buki, Renata Szalai, Adrienn Pinter, Kinga Hadzsiev, Bela Melegh, Tibor Rauch, Judit Bene   +6 more
wiley   +1 more source

Loss‐function mutants of OsCKX gene family based on CRISPR‐Cas systems revealed their diversified roles in rice

The Plant Genome, Volume 16, Issue 2, June 2023., 2023
Abstract Cytokinin (CTK) is an important plant hormone that promotes cell division, controls cell differentiation, and regulates a variety of plant growth and development processes. Cytokinin oxidase/dehydrogenase (CKX) is an irreversible cytokinin‐degrading enzyme that affects plant growth and development by regulating the dynamic balance of CTKs ...
Xuelian Zheng, Shuting Zhang, Yanling Liang, Rui Zhang, Li Liu, Pengchen Qin, Zhe Zhang, Yan Wang, Jianping Zhou, Xu Tang, Yong Zhang   +10 more
wiley   +1 more source

PCR-SSCP analysis in detecting point mutations targeting rpoB, katG and inhA genes for determining multi-drug resistance in Mycobacterium bovis and Mycobacterium tuberculosis strains

Indian Journal of Animal Sciences, 2014
The usefulness of polymerase chain reaction-single stranded confirmation polymorphism (PCR-SSCP) for determination of rifampicin and isoniazid resistance in Mycobacterium tuberculosis and M.
ALEX KIDANGAN, RISHENDRA VERMA
doaj   +1 more source

Multiple fluorescence-based PCR-SSCP analysis with postlabeling. [PDF]

Genome Research, 1995
Multiple fluorescence-based PCR single-strand conformation polymorphism (MF-PCR-SSCP) with postlabeling was developed. The target sequence was amplified by PCR using unlabeled primers. Free dNTPs were removed from the amplified products by ethanol precipitation.
H, Iwahana, K, Adzuma, Y, Takahashi, R, Katashima, K, Yoshimoto, M, Itakura   +5 more
openaire   +2 more sources

PCR-SSCCP analysis in detecting point mutations targeting rpoB, katG and inhA genes for determining multi-drug resistance in Mycobacterium bovis and Mycobacterium tuberculosis strains

Indian Journal of Animal Sciences, 2014
The usefulness of polymerase chain reaction-single stranded confirmation polymorphism (PCR-SSCP) for determination of rifampicin and isoniazid resistance in Mycobacterium tuberculosis and M.
ALEX KIDANGAN, RISHENDRA VERMA
doaj   +1 more source

Detection of rifampin resistance patterns in Mycobacterium tuberculosis strains isolated in Iran by polymerase chain reaction-single-strand conformation polymorphism and direct sequencing methods

Memorias do Instituto Oswaldo Cruz, 2006
Mutations in the rpoB locus confer conformational changes leading to defective binding of rifampin (RIF) to rpoB and consequently resistance in Mycobacterium tuberculosis.
Bahram Nasr Isfahani, Akbar Tavakoli, Mansoor Salehi, Mehdi Tazhibi   +3 more
doaj   +1 more source

rpoB Gene Mutations in Rifampin-Resistant Mycobacterium tuberculosis Identified by Polymerase Chain Reaction Single-Stranded Conformational Polymorphism

Emerging Infectious Diseases, 2001
The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results.
Miriam Bobadilla-del-Valle, Alfredo Ponce-de-Leon, Catalina Arenas-Huertero, Gilberto Vargas-Alarcon, Midori Kato-Maeda, Peter M. Small, Patricia Couary, Guillermo M. Ruiz-Palacios, Jose Sifuentes-Osornio   +8 more
doaj   +1 more source

Polymorphisms in swine candidate genes for meat quality detected by PCR-SSCP

Italian Journal of Animal Science, 2010
In order to identify SNPs in seven candidate genes for meat quality, PCR-SSCP experiments were carried out. Statistical analyses in a population of 240 individuals identified the animals constituting the extreme tails of the Gaussian distribution for ...
Stefania Chessa, Bianca Castiglioni, Giulio Pagnacco, Daniela Rignanese, Marta Raschetti   +4 more
doaj   +1 more source

PCR Mutagenesis-Based Method for Generation of Positive Controls for SSCP Analysis

BioTechniques, 1997
We have developed a primer-mediated PCR mutagenesis-based method for the generation of positive controls to test the sensitivity of single-strand conformation polymorphism (SSCP) or any other PCRbased mutation screening method. This technique is based on
A. Ruiz, G. Antiñolo, S. Borrego, B. Sánchez, J. Sánchez   +4 more
doaj   +1 more source