Inducible expression of (pp)pGpp synthetases in Staphylococcus aureus is associated with activation of stress response genes [PDF]
PLoS Genetics, 2020 The stringent response is characterized by the synthesis of the messenger molecules pppGpp, ppGpp or pGpp (here collectively designated (pp)pGpp). The phenotypic consequences resulting from (pp)pGpp accumulation vary among species and can be mediated by ...
Christoph Mayer +2 more
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The nucleotide pGpp acts as a third alarmone in Bacillus, with functions distinct from those of (p)ppGpp [PDF]
Nature Communications, 2020 Nucleotides pppGpp and ppGpp regulate bacterial responses to nutritional and other stresses, while the potential roles of the related pGpp are unclear. Here, Yang et al. systematically identify proteins interacting with these nucleotides in Bacillus, and
Daniel Amador-Noguez +2 more
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Direct detection of stringent alarmones (pp)pGpp using malachite green [PDF]
Microbial CellThe alarmone (p)ppGpp serves as the signalling molecule for the bacterial universal stringent response and plays a crucial role in bacterial virulence, persistence, and stress adaptation. Consequently, there is a significant focus on developing new drugs
Dominik Rejman +2 more
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Sound the (Smaller) Alarm: The Triphosphate Magic Spot Nucleotide pGpp [PDF]
Infection and Immunity, 2023 It has recently become evident that the bacterial stringent response is regulated by a triphosphate alarmone (pGpp) as well as the canonical tetra- and pentaphosphate alarmones ppGpp and pppGpp [together, (p)ppGpp]. Often dismissed in the past as an artifact or degradation product, pGpp has been confirmed as a deliberate endpoint of multiple synthetic ...
Erin B Purcell
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PgpP is a broadly conserved phosphatase required for phosphatidylglycerol lipid synthesis [PDF]
Proceedings of the National Academy of Sciences of the United States of AmericaThe cytoplasmic membrane of bacteria is composed of a phospholipid bilayer made up of a diverse set of lipids. Phosphatidylglycerol (PG) is one of the principal constituents and its production is essential for growth in many bacteria. All the enzymes required for PG biogenesis in Escherichia coli
David Rúdner +2 more
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Small Alarmone Synthetase SasA Expression Leads to Concomitant Accumulation of pGpp, ppApp, and AppppA in Bacillus subtilis [PDF]
Frontiers in Microbiology, 2020 (p)ppGpp is a highly conserved bacterial alarmone which regulates many aspects of cellular physiology and metabolism. In Gram-positive bacteria such as B.
Daniel Amador-Noguez +2 more
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The stringent response and physiological roles of (pp)pGpp in bacteria [PDF]
Nature Reviews Microbiology, 2020 The stringent response is a stress signalling system mediated by the alarmones guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp) in response to nutrient deprivation. Recent research highlights the complexity and broad range of functions that these alarmones control. This Review provides an update on our current understanding of the
Rebecca Corrigan +2 more
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From (p)ppGpp to (pp)pGpp: Characterization of Regulatory Effects of pGpp Synthesized by the Small Alarmone Synthetase of Enterococcus faecalis [PDF]
Journal of Bacteriology, 2015 ABSTRACT The bacterial stringent response (SR) is a conserved stress tolerance mechanism that orchestrates physiological alterations to enhance cell survival. This response is mediated by the intracellular accumulation of the alarmones pppGpp and ppGpp, collectively called (p)ppGpp. In Enterococcus faecalis
Dominik Rejman +2 more
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Photoaffinity Capture Compounds to Profile the Magic Spot Nucleotide Interactomes. [PDF]
Angew Chem Int Ed Engl, 2022 Molecular fishing: A family of trifunctional photoaffinity capture compounds enables the identification of putative Magic Spot Nucleotide receptors by a chemoproteomics approach. The resulting datasets cover several bacterial species, correlate with distinct nucleotide phosphorylation patterns and represent high‐value starting points for future ...
Haas TM +12 more
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Stringent Response in Mycobacteria: From Biology to Therapeutic Potential [PDF]
Pathogens, 2021 Mycobacterium tuberculosis is a human pathogen that can thrive inside the host immune cells for several years and cause tuberculosis. This is due to the propensity of M.
Andaleeb Sajid +2 more
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