The stringent response and physiological roles of (pp)pGpp in bacteria [PDF]
Nature Reviews Microbiology, 2020 The stringent response is a stress signalling system mediated by the alarmones guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp) in response to nutrient deprivation. Recent research highlights the complexity and broad range of functions that these alarmones control. This Review provides an update on our current understanding of the
Sophie E Irving +2 more
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From (p)ppGpp to (pp)pGpp: Characterization of Regulatory Effects of pGpp Synthesized by the Small Alarmone Synthetase of Enterococcus faecalis [PDF]
Journal of Bacteriology, 2015 ABSTRACT The bacterial stringent response (SR) is a conserved stress tolerance mechanism that orchestrates physiological alterations to enhance cell survival. This response is mediated by the intracellular accumulation of the alarmones pppGpp and ppGpp, collectively called (p)ppGpp. In Enterococcus faecalis
Anthony O Gaca +2 more
exaly +4 more sources
The Ps and Qs of alarmone synthesis in Staphylococcus aureus. [PDF]
PLoS ONE, 2019 During the stringent response, bacteria synthesize guanosine-3',5'-bis(diphosphate) (ppGpp) and guanosine-5'-triphosphate 3'-diphosphate (pppGpp), which act as secondary messengers to promote cellular survival and adaptation.
Ning Yang +5 more
doaj +3 more sources
Unique Features of Alarmone Metabolism in Clostridioides difficile [PDF]
Journal of Bacteriology, 2022 The “magic spot” alarmones (pp)pGpp, previously implicated in Clostridioides difficile antibiotic survival, are synthesized by the RelA-SpoT homolog (RSH) of C. difficile (RSH(Cd)) and RelQ(Cd).
Erin B Purcell
exaly +4 more sources
Photoaffinity Capture Compounds to Profile the Magic Spot Nucleotide Interactomes. [PDF]
Angew Chem Int Ed Engl, 2022 Molecular fishing: A family of trifunctional photoaffinity capture compounds enables the identification of putative Magic Spot Nucleotide receptors by a chemoproteomics approach. The resulting datasets cover several bacterial species, correlate with distinct nucleotide phosphorylation patterns and represent high‐value starting points for future ...
Haas TM +12 more
europepmc +5 more sources
Metabolic Promiscuity of an Orphan Small Alarmone Hydrolase Facilitates Bacterial Environmental Adaptation [PDF]
mBio, 2022 Small alarmone hydrolases (SAHs) are alarmone metabolizing enzymes found in both metazoans and bacteria. In metazoans, the SAH homolog Mesh1 is reported to function in cofactor metabolism by hydrolyzing NADPH to NADH.
Danny K. Fung +7 more
doaj +2 more sources
Functional Characterization of Small Alarmone Synthetase and Small Alarmone Hydrolase Proteins from Treponema denticola [PDF]
Microbiology Spectrum, 2023 The stringent response enables bacteria to survive nutrient starvation, antibiotic challenge, and other threats to cellular survival. Two alarmone (magic spot) second messengers, guanosine pentaphosphate (pppGpp) and guanosine tetraphosphate (ppGpp ...
Miao Wang +3 more
doaj +2 more sources
The extended (p)ppGpp family: New dimensions in Stress response [PDF]
Current Research in Microbial Sciences, 2021 Second messenger (p)ppGpp mediated stress response plays a crucial role in bacterial persistence and multiple drug resistance. In E. coli, (p)ppGpp binds to RNA polymerase and upregulates the transcription of genes essential for stress response while ...
Kirtimaan Syal +2 more
doaj +2 more sources
Crosstalk between guanosine nucleotides regulates cellular heterogeneity in protein synthesis during nutrient limitation. [PDF]
PLoS Genetics, 2022 Phenotypic heterogeneity of microbial populations can facilitate survival in dynamic environments by generating sub-populations of cells that may have differential fitness in a future environment.
Simon Diez +3 more
doaj +2 more sources
Effective Biosorption of Phosphate from Water Using Fe(III)-Loaded Pomegranate Peel [PDF]
Chemical and Biochemical Engineering Quarterly, 2023 Removal of phosphate from wastewater is necessary for the safety of public health and environmental protection. The present study used an easily available and affordable biosorbent obtained from the pomegranate peel for the excision of phosphate from ...
N. Bashyal +6 more
doaj +2 more sources