Results 11 to 20 of about 192,633 (279)

Recognizing Phosphatidylinositol 3-Phosphate [PDF]

Cell, 2001
Phosphatidylinositol 3-phosphate directs the endosomal localization of regulatory proteins by binding to FYVE and PX domains. New structures of these domains complexed with the phosphoinositide headgroup show how interactions with phosphate and hydroxyl groups differentiate this lipid from all others.
Misra, Saurav, Miller, Gregory J, Hurley, James H   +2 more
openaire   +4 more sources

Phosphatidylinositol 3-phosphate regulates iron transport via PI3P-binding CgPil1 protein

Cell Reports, 2023
Summary: Iron homeostasis, which is pivotal to virulence, is regulated by the phosphatidylinositol 3-kinase CgVps34 in the human fungal pathogen Candida glabrata.
Fizza Askari, Bhogadi Vasavi, Rupinder Kaur   +2 more
doaj   +3 more sources

Phosphatidylinositol-3-phosphate regulates response of cells to proteotoxic stress [PDF]

The International Journal of Biochemistry & Cell Biology, 2016
Human Nedd4 ubiquitin ligase, or its variants, inhibit yeast cell growth by disturbing the actin cytoskeleton organization and dynamics, and lead to an increase in levels of ubiquitinated proteins.
Ayscough, K., Bala, K., Flis, K., Grynberg, M., Kaliszewski, P., Kaminska, J., Polak, A., Rzepnikowska, W., Sienko, M., Soczewka, P., Urbanek, A., Zoladek, T.   +11 more
core   +5 more sources

The phosphatidylinositol-3-phosphate 5-kinase inhibitor apilimod blocks filoviral entry and infection. [PDF]

PLoS Neglected Tropical Diseases, 2017
Phosphatidylinositol-3-phosphate 5-kinase (PIKfyve) is a lipid kinase involved in endosome maturation that emerged from a haploid genetic screen as being required for Ebola virus (EBOV) infection.
Elizabeth A Nelson, Julie Dyall, Thomas Hoenen, Alyson B Barnes, Huanying Zhou, Janie Y Liang, Julia Michelotti, William H Dewey, Lisa Evans DeWald, Richard S Bennett, Patrick J Morris, Rajarshi Guha, Carleen Klumpp-Thomas, Crystal McKnight, Yu-Chi Chen, Xin Xu, Amy Wang, Emma Hughes, Scott Martin, Craig Thomas, Peter B Jahrling, Lisa E Hensley, Gene G Olinger, Judith M White   +23 more
doaj   +2 more sources

Structural, thermodynamic, and phosphatidylinositol 3-phosphate binding properties of Phafin2. [PDF]

Protein Sci, 2017
AbstractPhafin2 is a phosphatidylinositol 3‐phosphate (PtdIns(3)P) binding protein involved in the regulation of endosomal cargo trafficking and lysosomal induction of autophagy. Binding of Phafin2 to PtdIns(3)P is mediated by both its PH and FYVE domains.
Tang TX, Jo A, Deng J, Ellena JF, Lazar IM, Davis RM, Capelluto DG.   +6 more
europepmc   +4 more sources

Protocol for determination of phosphatidylinositol 3-phosphate levels and localization in Candida glabrata by confocal microscopy [PDF]

STAR Protocols
Summary: Phosphatidylinositol 3-phosphate (PI3P) levels govern membrane trafficking in Candida glabrata. Here, we present a confocal imaging-based protocol for PI3P localization analysis using the GFP-FYVE (found in Fab1, YOTB, Vac1, and EEA1) fusion ...
Fizza Askari, Rupinder Kaur
doaj   +2 more sources

Insulin induces phosphatidylinositol-3-phosphate formation through TC10 activation [PDF]

The EMBO Journal, 2003
Phosphatidylinositol-3-phosphate (PtdIns-3-P) is considered as a lipid constitutively present on endosomes; it does not seem to have a dynamic role in signalling. In contrast, phosphatidylinositol-3,4,5-trisphosphate (PtdIns-3,4,5-P(3)) plays a crucial role in different signalling pathways including translocation of the glucose transporter protein ...
Maffucci T, Brancaccio A, Piccolo E, Stein RC, Falasca M   +4 more
openaire   +5 more sources

Time-resolved ultrastructural detection of phosphatidylinositol 3-phosphate. [PDF]

J Histochem Cytochem, 2010
Phosphatidylinositol 3-phosphate [Ptdlns(3)P] playsan important role in recruitment of various effector proteins in the endocytic and autophagic pathways. In an attempt to follow the distribution of Ptdlns(3)P at the ultrastructural level, we are using the Fab1, YOTB, Vac1, and EEA1 (FYVE) domain, which is a zinc finger motif specifically binding to ...
Stuffers S, Malerød L, Schink KO, Corvera S, Stenmark H, Brech A.   +5 more
europepmc   +4 more sources

Understanding phosphatidylinositol-3-phosphate dynamics during autophagosome biogenesis. [PDF]

Autophagy, 2012
Autophagosomes, the hallmark of autophagy, are double-membrane vesicles sequestering cytoplasmic components. They are generated at the phagophore assembly site (PAS), the phagophore being the precursor structure of these carriers. According to the current model, autophagosomes result from the elongation and reorganization of membranes at the PAS ...
Cebollero E, van der Vaart A, Reggiori F.   +2 more
europepmc   +4 more sources

How Atg18 and the WIPIs sense phosphatidylinositol 3-phosphate. [PDF]

Autophagy, 2012
The key autophagic lipid sensors are Atg18 in yeast and the WIPI proteins in mammals. Atg18 and the WIPIs belong to the PROPPIN family of proteins. PROPPINs are seven- bladed β-propellers that bind to phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P2].
Baskaran S, Ragusa MJ, Hurley JH.
europepmc   +4 more sources