The myotubularin MTMR4 regulates phagosomal phosphatidylinositol 3-phosphate turnover and phagocytosis. [PDF]
J Biol Chem, 2019 Macrophage phagocytosis is required for effective clearance of invading bacteria and other microbes. Coordinated phosphoinositide signaling is critical both for phagocytic particle engulfment and subsequent phagosomal maturation to a degradative organelle.
Sheffield DA +8 more
europepmc +4 more sources
Two-site recognition of phosphatidylinositol 3-phosphate by PROPPINs in autophagy. [PDF]
Mol Cell, 2012 Macroautophagy is essential to cell survival during starvation and proceeds by the growth of a double-membraned phagophore, which engulfs cytosol and other substrates. The synthesis and recognition of the lipid phosphatidylinositol 3-phosphate, PI(3)P, is essential for autophagy.
Baskaran S +3 more
europepmc +4 more sources
Determination of Cellular Phosphatidylinositol-3-phosphate (PI3P) Levels Using a Fluorescently Labelled Selective PI3P Binding Domain (PX) [PDF]
Bio-Protocol, 2016 The lipid Phosphatidylinositol-3-phosphate [PtdIns3P or PI(3)P] plays many membrane trafficking roles and is primarily produced by the Class III PI3K, VPS34. Determining the level of cellular PI(3)P however can be complex.
Michael Munson, Ian Ganley
doaj +2 more sources
Phosphatidylinositol-3-phosphate clearance plays a key role in autophagosome completion. [PDF]
Curr Biol, 2012 The biogenesis of autophagosomes, the hallmark of autophagy, depends on the function of the autophagy-related (Atg) proteins and the generation of phosphatidylinositol-3-phosphate (PtdIns3P) at the phagophore assembly site (PAS), the location where autophagosomes arise.
Cebollero E +6 more
europepmc +6 more sources
Identification of Key Phospholipids That Bind and Activate Atypical PKCs
Biomedicines, 2021 PKCζ and PKCι/λ form the atypical protein kinase C subgroup, characterised by a lack of regulation by calcium and the neutral lipid diacylglycerol. To better understand the regulation of these kinases, we systematically explored their interactions with ...
Suresh Velnati +10 more
doaj +1 more source
The Arabidopsis thaliana immunophilin ROF1 directly interacts with PI(3)P and PI(3,5)P2 and affects germination under osmotic stress. [PDF]
PLoS ONE, 2012 A direct interaction of the Arabidopsis thaliana immunophilin ROF1 with phosphatidylinositol-3-phosphate and phosphatidylinositol-3,5-bisphosphate was identified using a phosphatidylinositol-phosphate affinity chromatography of cell suspension extracts ...
Debora Karali +4 more
doaj +1 more source
Nature Communications, 2023 Exosomes are secreted to the extracellular milieu when multivesicular endosomes (MVEs) dock and fuse with the plasma membrane. However, MVEs are also known to fuse with lysosomes for degradation.
Di-Ao Liu +11 more
doaj +1 more source
Lipid kinases PIP5Ks and PIP4Ks: potential drug targets for breast cancer
Frontiers in Oncology, 2023 Phosphoinositides, a small group of lipids found in all cellular membranes, have recently garnered heightened attention due to their crucial roles in diverse biological processes and different diseases. Among these, phosphatidylinositol 4,5-bisphosphate (
Yue Jin, Jian Xue
doaj +1 more source
The phosphatidylinositol 3‐phosphate‐binding FYVE finger [PDF]
FEBS Letters, 2002 The FYVE zinc finger domain is conserved from yeast (five proteins) to man (27 proteins). It functions in the membrane recruitment of cytosolic proteins by binding to phosphatidylinositol 3‐phosphate (PI3P), which is found mainly on endosomes. Here we review recent work that sheds light on the targeting of FYVE finger proteins to PI3P‐containing ...
Stenmark, Harald +2 more
openaire +2 more sources
Crystal Structure of Human Myotubularin-Related Protein 1 Provides Insight into the Structural Basis of Substrate Specificity. [PDF]
PLoS ONE, 2016 Myotubularin-related protein 1 (MTMR1) is a phosphatase that belongs to the tyrosine/dual-specificity phosphatase superfamily. MTMR1 has been shown to use phosphatidylinositol 3-monophosphate (PI(3)P) and/or phosphatidylinositol 3,5-bisphosphate (PI(3,5 ...
Seoung Min Bong +9 more
doaj +1 more source