Plk4-Induced Centriole Biogenesis in Human Cells [PDF]
Developmental Cell, 2007 We show that overexpression of Polo-like kinase 4 (Plk4) in human cells induces centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole. This provided an opportunity for dissecting centriole assembly and characterizing assembly intermediates.
Kleylein-Sohn, Julia +5 more
openaire +3 more sources
GCP6 is a substrate of Plk4 and required for centriole duplication [PDF]
Journal of Cell Science, 2012 Centriole duplication occurs once per cell cycle and requires Plk4, a member of the Polo-like kinase family. A key component of the centrosome is the γ-tubulin ring complex (γ-TuRC) that nucleates microtubules. GCP6 is a member of the γ-TuRC, but its role in human cells and the regulation of its functions remain unclear.
Ramona, Bahtz +6 more
openaire +2 more sources
On the regulation of centriole duplication in human cells : exploring the interactions of polo-like kinase 4 with the centrosomal proteins Cep192 and STIL [PDF]
, 2015 Centrioles duplicate once in each cell cycle to give rise to two centrosomes that form the spindle poles during mitosis. Aberrant centriole duplication can result in the formation of supernumerary centrosomes, leading to incorrect spindle assembly and ...
Gabryjonczyk, Anna-Maria
core +1 more source
Control of centriole numbers by Plk4 autophosphorylation and βTrCP-mediated degradation [PDF]
, 2010 Proper centrosome numbers are imperative for faithful cell division, as aberrant centrosome numbers can lead to chromosomal instability, a hallmark of cancer development (Nigg 2002; Ganem et al., 2009).
Guderian, Gernot
core +1 more source
Downregulation of polo-like kinase 4 in hepatocellular carcinoma associates with poor prognosis. [PDF]
PLoS ONE, 2012 Polo-like kinase 4 (PLK4), belonging to serine/threonine kinase family, is critical for centriole replication and cell cycle progression. PLK4 has been proposed as a tumor suppressor in hepatocellular carcinoma (HCC).
Lili Liu +5 more
doaj +1 more source
A self-assembled cylindrical platform for Plk4-induced centriole biogenesis [PDF]
Open Biology, 2020 The centrosome, a unique membraneless multiprotein organelle, plays a pivotal role in various cellular processes that are critical for promoting cell proliferation.
Kyung S. Lee +4 more
doaj +1 more source
Investigation of human centrosomes - with a special focus on the function of Cep152 and Cep192 in centriole duplication [PDF]
, 2012 Centrioles are symmetrical, barrel-shaped, microtubule-based structures, that serve as building platforms for the formation of centrosomes and cilia. In dividing cells, they recruit a matrix of proteins called pericentriolar material (PCM) to form the ...
Sonnen, Katharina Friederike
core +1 more source
Asterless amplifies Plk4 [PDF]
Journal of Cell Biology, 2015 ![Figure][1] Superresolution microscopy shows the structure of stable aggregates formed by overexpressed Plk4 (green) and Asl (red). [Klebba and Galletta et al.][2] reveal that the scaffold protein Asterless (Asl) regulates centriole duplication by controlling turnover of the kinase ...
openaire +1 more source
CFI-400945 is not a selective cellular PLK4 inhibitor [PDF]
Proceedings of the National Academy of Sciences, 2018 In the PNAS paper “Polo-like kinase 4 inhibition produces polyploidy and apoptotic death of lung cancers,” Kawakami et al. (1) treat lung cancer cell lines and mouse tumor models with the small molecule CFI-400945 and interpret its effects as being solely due to inhibition of Polo-like kinase 4 (PLK4), the kinase essential for centrosome duplication (2)
Karen Oegema +4 more
openaire +2 more sources
Mechanistic study on PLK4 regulation of invasion, proliferation and apoptosis in oral squamous cell carcinoma [PDF]
Zhongguo aizheng zazhiBackground and purpose: Polo-like kinase 4 (PLK4) is a cell cycle regulatory protein, which is closely related to the occurrence and development of a variety of malignant tumors.
QIN Huan, WANG Jie, YANG Jie, HUA Yingjie, QU Huijuan, JI Honghai
doaj +3 more sources