Results 41 to 50 of about 53,758 (224)
Analysis of Oct4-dependent transcriptional networks regulating self-renewal and pluripotency in human embryonic stem cells [PDF]
, 2006 The POU domain transcription factor OCT4 is a key regulator of pluripotency in the early mammalian embryo and is highly expressed in the inner cell mass of the blastocyst. Consistent with its essential role in maintaining pluripotency, Oct4 expression is
Adjaye, J. +8 more
core +2 more sources
microRNA-mediated regulation of microRNA machinery controls cell fate decisions
eLife, 2021 microRNAs associate with Argonaute proteins, forming the microRNA-induced silencing complex (miRISC), to repress target gene expression post-transcriptionally.
Qiuying Liu +4 more
doaj +1 more source
Speeding to Pluripotency [PDF]
Cell, 2014 Finding a cell that reprograms in a nonstochastic manner without genetic manipulation has proven elusive. In this issue, Guo et al. report the identification of a cell defined by an ultrafast cycle whose progeny reprogram in a synchronous and rapid manner.
Chenoweth, Josh G., McKay, Ronald D.
openaire +2 more sources
Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]
, 2013 Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra +6 more
core +2 more sources
An Endogenously Tagged Fluorescent Fusion Protein Library in Mouse Embryonic Stem Cells
Stem Cell Reports, 2017 Embryonic stem cells (ESCs), with their dual capacity to self-renew and differentiate, are commonly used to study differentiation, epigenetic regulation, lineage choices, and more. Using non-directed retroviral integration of a YFP/Cherry exon into mouse
Arigela Harikumar +11 more
doaj +1 more source
Cells, 2022 Pluripotency maintenance and exit in embryonic stem cells is a focal topic in stem cell biology. However, the effects of screening under very stringent culture conditions (e.g., differentiation medium, no leukemia inhibitory factor, no chemical ...
Chen Gao +9 more
doaj +1 more source
Endogenous Wnt signalling in human embryonic stem cells generates an equilibrium of distinct lineage-specified progenitors. [PDF]
, 2012 The pluripotent nature of human embryonic stem cells (hESCs) makes them convenient for deriving therapeutically relevant cells. Here we show using Wnt reporter hESC lines that the cells are heterogeneous with respect to endogenous Wnt signalling activity.
Ardehali, Reza +4 more
core +1 more source
Stem Cell Reports, 2017 Promyelocytic leukemia protein (PML), the main constituent of PML nuclear bodies, regulates various physiological processes in different cell types. However, little is known about its functions in embryonic stem cells (ESC).
Christiana Hadjimichael +7 more
doaj +1 more source
Systematic optimization of human pluripotent stem cells media using Design of Experiments. [PDF]
, 2015 Human pluripotent stem cells (hPSC) are used to study the early stages of human development in vitro and, increasingly due to somatic cell reprogramming, cellular and molecular mechanisms of disease.
Chailangkarn, Thanathom +2 more
core +1 more source
Frontiers in Oncology, 2014 The striking similarity displayed at the mechanistic level between tumorigenesis and the generation of induced pluripotent stem cells and the fact that genes and pathways relevant for embryonic development are reactivated during tumor progression ...
Juan Manuel eIglesias +20 more
doaj +1 more source