Results 251 to 260 of about 7,884,495 (281)
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Post-transcriptional gene-silencing: RNAs on the attack or on the defense?
BioEssays, 2000Post-transcriptional gene-silencing (PTGS) was first discovered in plants and results from the sequence-specific degradation of RNA. Degradation can be activated by introducing transgenes, RNA viruses or DNA sequences that are homologous to expressed genes. A similar RNA degradation mechanism which is inducible by double-stranded RNA (dsRNAs), has been
Sijen, L.M.T., Kooter, J.M.
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The diversity of post-transcriptional gene silencing mediated by small silencing RNAs in plants
Essays in Biochemistry, 2020Abstract In plants, post-transcriptional gene silencing (PTGS) tightly regulates development, maintains genome stability and protects plant against foreign genes. PTGS can be triggered by virus infection, transgene, and endogenous transcript, thus commonly serves as an RNA-based immune mechanism.
Huijuan Tan, Bosheng Li, Hongwei Guo
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Graft transmission of induced and spontaneous post-transcriptional silencing of chitinase genes [PDF]
Plant Journal, 2001 SummarySense and antisense tobacco chitinase (CHN) transgenes, Luciferase‐CHN transcriptional fusions, and promoterless CHN cDNAs were introduced biolistically into CHN transformants of tobacco that never exhibit spontaneous gene silencing. All of the constructs tested induced systemic silencing of the resident CHN transgene and endogenes.
Frederick Meins, F Meins
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Post-transcriptional gene silencing: conservation and sequences
Trends in Plant Science, 2000Gene expression is controlled by a variety of mechanisms. Post-transcriptional gene silencing (PTGS) is one mechanism that degrades specific messenger RNAs and thereby reduces the expression of a specific gene. PTGS has many names: cosuppression in plants, quelling in fungi and RNA interference in animals, but in all cases, degraded mRNA decreases gene
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2022
Loss-of-function analyses are essential to dissect the complex nature of biological processes, including gametogenesis. Virus-induced gene silencing (VIGS) has been widely used in crop species as an amenable and rapid way to generate gene knockdowns. As a transient assay, VIGS circumvents the generation of stable transgenic lines through laborious and ...
Wang, Zhengming +3 more
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Loss-of-function analyses are essential to dissect the complex nature of biological processes, including gametogenesis. Virus-induced gene silencing (VIGS) has been widely used in crop species as an amenable and rapid way to generate gene knockdowns. As a transient assay, VIGS circumvents the generation of stable transgenic lines through laborious and ...
Wang, Zhengming +3 more
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RNA viruses as inducers, suppressors and targets of post-transcriptional gene silencing
Plant Molecular Biology, 2000Post-transcriptional gene silencing (PTGS) is a fundamental regulatory mechanism operating in diverse types of organisms, but the cellular components of the gene silencing machinery and the regulation of the process are not understood. Recent findings that cytoplasmically replicating RNA viruses act as both targets and inducers of PTGS has led to the ...
Gail J Pruss, Vicki Vance
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RNA degradation and models for post-transcriptional gene silencing
Plant Molecular Biology, 2000Post-transcriptional gene silencing (PTGS) is a form of stable but potentially reversible epigenetic modification, which frequently occurs in transgenic plants. The interaction in trans of genes with similar transcribed sequences results in sequence-specific degradation of RNAs derived from the genes involved.
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The Design and Exogenous Delivery of siRNA for Post-transcriptional Gene Silencing
Journal of Drug Targeting, 2004RNA interference (RNAi) is a natural cellular process that effects post-transcriptional gene silencing in eukaryotic systems. Small interfering RNA (siRNA) molecules are the key intermediaries in this process which when exogenously administered can inhibit or "silence" the expression of any given target gene. Thus, siRNA molecules hold great promise as
Ian R, Gilmore +4 more
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Post-transcriptional Gene Silencing and Translation in Giardia
2011The control of gene expression in Giardia lamblia includes several mechanisms already described in higher eukaryotes, but with some interesting features for this early-branching organism. Here we describe two gene expression control systems in Giardia, posttranscriptional gene silencing (PTGS) and translation, and the close interaction between them ...
Gargantini, Pablo Ruben +2 more
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Conservation of transgene-induced post-transcriptional gene silencing in plants and fungi
Trends in Plant Science, 1997Different types of post-transcriptional transgene-induced gene silencing have been shown to occur in Neurospora (quelling) and plants (co-suppression). Common mechanistic features include triggering by duplicated coding sequences, simultaneous silencing of the transgene and endogenous genes, reversibility of silencing and post-transcriptional ...
COGONI, Carlo, MACINO, Giuseppe
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