Results 261 to 270 of about 255,778 (299)
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Post-transcriptional gene silencing by siRNAs and miRNAs
Current Opinion in Structural Biology, 2005Recent years have seen a rapid increase in our understanding of how double-stranded RNA (dsRNA) and 21- to 25-nucleotide small RNAs, microRNAs (miRNAs) and small interfering RNAs (siRNAs), control gene expression in eukaryotes. This RNA-mediated regulation generally results in sequence-specific inhibition of gene expression; this can occur at levels as
Witold, Filipowicz +3 more
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Post-transcriptional gene silencing activity of human GIGYF2
Biochemical and Biophysical Research Communications, 2016In mammalian post-transcriptional gene silencing, the Argonaute protein AGO2 indirectly recruits translation inhibitors, deadenylase complexes, and decapping factors to microRNA-targeted mRNAs, thereby repressing mRNA translation and accelerating mRNA decay.
Marie-Hélène, Kryszke +4 more
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2022
Loss-of-function analyses are essential to dissect the complex nature of biological processes, including gametogenesis. Virus-induced gene silencing (VIGS) has been widely used in crop species as an amenable and rapid way to generate gene knockdowns. As a transient assay, VIGS circumvents the generation of stable transgenic lines through laborious and ...
Wang, Zhengming +3 more
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Loss-of-function analyses are essential to dissect the complex nature of biological processes, including gametogenesis. Virus-induced gene silencing (VIGS) has been widely used in crop species as an amenable and rapid way to generate gene knockdowns. As a transient assay, VIGS circumvents the generation of stable transgenic lines through laborious and ...
Wang, Zhengming +3 more
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Spreading of post‐transcriptional gene silencing along the target gene promotes systemic silencing
The Plant Journal, 2004SummaryTransitive silencing and grafting‐induced gene silencing phenomena were combined to investigate whether a primary target β‐glucuronidase (gus) gene could promote the generation of systemic transitive silencing signals. Tobacco plants with hemizygous or homozygous silencer locus and in trans silenced primary target were used as a source of post ...
Rubén Darío, García-Pérez +2 more
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The diversity of post-transcriptional gene silencing mediated by small silencing RNAs in plants
Essays in Biochemistry, 2020Abstract In plants, post-transcriptional gene silencing (PTGS) tightly regulates development, maintains genome stability and protects plant against foreign genes. PTGS can be triggered by virus infection, transgene, and endogenous transcript, thus commonly serves as an RNA-based immune mechanism.
Huijuan Tan, Bosheng Li, Hongwei Guo
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Post-transcriptional gene silencing: conservation and sequences
Trends in Plant Science, 2000Gene expression is controlled by a variety of mechanisms. Post-transcriptional gene silencing (PTGS) is one mechanism that degrades specific messenger RNAs and thereby reduces the expression of a specific gene. PTGS has many names: cosuppression in plants, quelling in fungi and RNA interference in animals, but in all cases, degraded mRNA decreases gene
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Intrinsic direct repeats generate consistent post‐transcriptional gene silencing in tobacco
The Plant Journal, 2002SummaryIt is well documented that transgenes with inverted repeats can efficiently trigger post‐transcriptional gene silencing (PTGS), presumably via a double stranded RNA induced by complementary sequences in their transcripts. We show here that transgenes with intrinsic direct repeats can also induce PTGS at a very high frequency (80–100%).
Chonglie, Ma, Amitava, Mitra
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Phosphorus starvation induces post-transcriptional CHS gene silencing in Petunia corolla
Plant Cell Reports, 2013The corolla of Petunia 'Magic Samba' exhibits unstable anthocyanin expression depending on its phosphorus content. Phosphorus deficiency enhanced post-transcriptional gene silencing of chalcone synthase - A in the corolla. Petunia (Petunia hybrida) 'Magic Samba' has unstable red-white bicolored corollas that respond to nutrient deficiency. We grew this
Munetaka, Hosokawa +10 more
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RNA degradation and models for post-transcriptional gene silencing
Plant Molecular Biology, 2000Post-transcriptional gene silencing (PTGS) is a form of stable but potentially reversible epigenetic modification, which frequently occurs in transgenic plants. The interaction in trans of genes with similar transcribed sequences results in sequence-specific degradation of RNAs derived from the genes involved.
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Post-transcriptional Gene Silencing and Translation in Giardia
2011The control of gene expression in Giardia lamblia includes several mechanisms already described in higher eukaryotes, but with some interesting features for this early-branching organism. Here we describe two gene expression control systems in Giardia, posttranscriptional gene silencing (PTGS) and translation, and the close interaction between them ...
Pablo R. Gargantini +2 more
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