Proline Dehydrogenase and Pyrroline 5 Carboxylate Dehydrogenase from Mycobacterium tuberculosis: Evidence for Substrate Channeling [PDF]
Pathogens, 2023 In Mycobacterium tuberculosis, proline dehydrogenase (PruB) and ∆1-pyrroline-5-carboxylate (P5C) dehydrogenase (PruA) are monofunctional enzymes that catalyze proline oxidation to glutamate via the intermediates P5C and L-glutamate-γ-semialdehyde.
Santosh Kumar +6 more
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Can proline dehydrogenase—a key enzyme involved in proline metabolism—be a novel target for cancer therapy? [PDF]
Frontiers in Oncology, 2023 Emerging evidence suggests that proline metabolism is important for regulating the survival and death of different types of cancer cells. Proline dehydrogenase (PRODH), an enzyme catalyzing proline catabolism, and the degradation products of proline by ...
Xiangyuan Xu +10 more
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Crystallographic Fragment Screening of a Bifunctional Proline Catabolic Enzyme Reveals New Inhibitor Templates for Proline Dehydrogenase and L-Glutamate-γ-semialdehyde Dehydrogenase [PDF]
MoleculesThe proline catabolic pathway consisting of proline dehydrogenase (PRODH) and L-glutamate-γ-semialdehyde (GSAL) dehydrogenase (GSALDH) catalyzes the four-electron oxidation of L-proline to L-glutamate. Chemical probes to these enzymes are of interest for
Kaylen R. Meeks +3 more
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Structure-affinity relationships of reversible proline analog inhibitors targeting proline dehydrogenase. [PDF]
Org Biomol Chem, 2022 PRODH plays a central role in the metabolic rewiring of cancer cells. Analysis of 18 proline analogs using kinetics and crystallography revealed an unexpected synergism between inhibitor ring size and hydrogen bonding to an active site water molecule.
Bogner AN, Tanner JJ.
europepmc +3 more sources
Collagen metabolism as a regulator of proline dehydrogenase/proline oxidase-dependent apoptosis/autophagy. [PDF]
Amino Acids, 2021 AbstractRecent studies on the regulatory role of amino acids in cell metabolism have focused on the functional significance of proline degradation. The process is catalysed by proline dehydrogenase/proline oxidase (PRODH/POX), a mitochondrial flavin-dependent enzyme converting proline into ∆1-pyrroline-5-carboxylate (P5C).
Palka J, Oscilowska I, Szoka L.
europepmc +4 more sources
NSAIDs Induce Proline Dehydrogenase/Proline Oxidase-Dependent and Independent Apoptosis in MCF7 Breast Cancer Cells. [PDF]
Int J Mol Sci, 2022 Non-steroidal anti-inflammatory drugs (NSAIDs) are considered in cancer therapy for their inhibitory effect on cyclooxygenase-2 (COX-2), which is overexpressed in most cancers. However, we found that NSAIDs as ligands of peroxisome proliferator-activated receptor-γ (PPARγ)-induced apoptosis independent of the COX-2 inhibition, and the process was ...
Kazberuk A +4 more
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Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine [PDF]
Animals, 2020 Proline was reported to improve sperm quality in rams, stallions, cynomolgus monkeys, donkeys, and canines during cryopreservation. However, the underlying mechanism remains unclear.
Chengwen Feng +9 more
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Photoinduced Covalent Irreversible Inactivation of Proline Dehydrogenase by S-Heterocycles. [PDF]
ACS Chem Biol, 2021 Proline dehydrogenase (PRODH) is a flavoenzyme that catalyzes the first step of proline catabolism, the oxidation of l-proline to Δ1-pyrroline-5-carboxylate. PRODH has emerged as a cancer therapy target because of its involvement in the metabolic reprogramming of cancer cells.
Campbell AC +6 more
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Proline dehydrogenase (oxidase) in cancer. [PDF]
Biofactors, 2012 AbstractProline dehydrogenase (oxidase, PRODH/POX), the first enzyme in the proline degradative pathway, plays a special role in tumorigenesis and tumor development. Proline metabolism catalyzed by PRODH/POX is closely linked with the tricarboxylic acid (TCA) cycle and urea cycle.
Liu W, Phang JM.
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Appropriate Activity Assays Are Crucial for the Specific Determination of Proline Dehydrogenase and Pyrroline-5-Carboxylate Reductase Activities [PDF]
Frontiers in Plant Science, 2020 Accumulation of proline is a widespread plant response to a broad range of environmental stress conditions including salt and osmotic stress. Proline accumulation is achieved mainly by upregulation of proline biosynthesis in the cytosol and by inhibition
Sandrine Lebreton +4 more
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