Results 41 to 50 of about 46,423 (228)

Proline dehydrogenase is essential for proline protection against hydrogen peroxide-induced cell death

Free Radical Biology and Medicine, 2012
Proline metabolism has an underlying role in apoptotic signaling that influences tumorigenesis. Proline is oxidized to glutamate in the mitochondria, with the rate-limiting step catalyzed by proline dehydrogenase (PRODH). PRODH expression is inducible by p53, leading to increased proline oxidation, reactive oxygen species formation, and induction of ...
Natarajan, Sathish Kumar, Zhu, Weidong, Liang, Xinwen, Zhang, Lu, Demers, Andrew, Zimmerman, Matthew C., Simpson, Melanie A., Becker, Donald F.   +7 more
openaire   +3 more sources

Involvement of the β3-α3 Loop of the Proline Dehydrogenase Domain in Allosteric Regulation of Membrane Association of Proline Utilization A [PDF]

Biochemistry, 2013
Proline utilization A (PutA) from Escherichia coli is a membrane-associated trifunctional flavoenzyme that catalyzes the oxidation of proline to glutamate and moonlights as a transcriptional regulator. As a regulatory protein, PutA represses transcription of the put regulon, which contains the genes encoding PutA and the proline transporter PutP.
Zhu, Weidong, Haile, Ashley M, Singh, Ranjan K, Larson, John D, Smithen, Danielle, Chan, Jie Y, Tanner, John J, Becker, Donald F   +7 more
openaire   +2 more sources

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Proline Dehydrogenase Activity of the Transcriptional Repressor PutA Is Required for Induction of the put Operon by Proline (∗)

Journal of Biological Chemistry, 1995
The proline utilization (put) operon from Salmonella typhimurium consists of the putP gene, encoding a proline transporter, and the putA gene, encoding an enzyme with both proline dehydrogenase and 1-pyrroline-5-carboxylate dehydrogenase activities.
A M, Muro-Pastor, S, Maloy
openaire   +2 more sources

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Proline–Nitrogen Metabolic Coordination Mediates Cold Priming-Induced Freezing Tolerance in Maize

Plants
Cold stress critically restricts maize seedling growth in Northeast China, yet the mechanism by which cold priming (CP) enhances cold tolerance through proline–nitrogen metabolic networks remains unclear.
Zhijia Gai, Lei Liu, Na Zhang, Jingqi Liu, Lijun Cai, Xu Yang, Ao Zhang, Pengfei Zhang, Junjie Ding, Yifei Zhang   +9 more
doaj   +1 more source

A bienzyme electrochemical biosensor for the detection of collagen l-hydroxyproline

Sensing and Bio-Sensing Research, 2015
l-hydroxyproline (l-Hyp: trans-4-hydroxy-l-proline) is commonly found at high concentrations in connective tissue proteins such as collagen. It is a remarkably useful molecular marker because variation in the level of l-Hyp is associated with various ...
Hiroaki Sakamoto, Kazuya Watanabe, Ayako Koto, Gaku Koizumi, Takenori Satomura, Seiya Watanabe, Shin-ichiro Suye   +6 more
doaj   +1 more source

Variability of leaf pubescence characteristics in transgenic tobacco lines with partial proline dehydrogenase gene suppression

Biologia Plantarum, 2022
Proline, an amino acid, plays an important role in plants, and it is involved in stress resistance and development. Earlier, to study the proline role in maintaining stress resistance in plants, we obtained genetically modified transgenic lines of ...
S.M. IBRAGIMOVA, M.A. GENAEV, A.V. KOCHETOV, D.A. AFONNIKOV   +3 more
doaj   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Cross Kingdom Metabolic Engineering Paradigm Elevating Sustainable Protein Production

Advanced Science, EarlyView.
ABSTRACT Confronting the dual crisis of escalating global protein demand and unsustainable agriculture necessitates transformative solutions. Here, we pioneer evolutionary insights from maize nitrogen optimization via asparagine synthetase (ASNS) to rewire metabolism in Pichia pastoris.
Yuanyuan Du, Changyu Pi, Kai Hong, Le Gao, Xin Wu   +4 more
wiley   +1 more source