Results 41 to 50 of about 3,788 (176)

Protein-Interaction Affinity Gradient Drives [4Fe–4S] Cluster Insertion in Human Lipoyl Synthase

Journal of the American Chemical Society, 2022
Human lipoyl synthase (LIAS) is an enzyme containing two [4Fe-4S] clusters (named FeSRS and FeSaux) involved in the biosynthesis of the lipoyl cofactor. The mechanism by which a [4Fe-4S] cluster is inserted into LIAS has thus far remained elusive. Here we show that NFU1 and ISCA1 of the mitochondrial iron-sulfur cluster assembly machinery, via forming ...
Saudino, Giovanni, Ciofi-Baffoni, Simone, Banci, Lucia   +2 more
openaire   +3 more sources

Cuproptosis predicts the risk and clinical outcomes of lung adenocarcinoma

Frontiers in Oncology, 2022
Copper is an essential microelement for the body and a necessary coregulator for enzymatic reactions, yet an unbalanced copper level promotes reactive oxidation and cytotoxicity, which ultimately induces cell death.
Qin Hu, Qin Hu, Runtian Wang, Huiyun Ma, Huiyun Ma, Zhouwei Zhang, Zhouwei Zhang, Qun Xue   +7 more
doaj   +1 more source

Glycine Cleavage System H Protein Is Essential for Embryonic Viability, Implying Additional Function Beyond the Glycine Cleavage System

Frontiers in Genetics, 2021
Glycine cleavage system H protein (GCSH) is a component of the glycine cleavage system (GCS), a conserved protein complex that acts to decarboxylate glycine.
Kit-Yi Leung, Sandra C. P. De Castro, Gabriel L. Galea, Andrew J. Copp, Nicholas D. E. Greene   +4 more
doaj   +1 more source

Expression, Lipoylation and Structure Determination of Recombinant Pea H‐Protein in Escherichia coli [PDF]

European Journal of Biochemistry, 1996
A synthetic gene encoding the entire mature H protein of the glycine decarboxylase complex from pea (Pisum sativum L.) was constructed and expressed in Escherichia coli. The recombinant H protein, which after the induction period constituted more than half of the E. coli protein, was found in a soluble form.
D, Macherel, J, Bourguignon, E, Forest, M, Faure, C, Cohen-Addad, R, Douce   +5 more
openaire   +2 more sources

Lipidomic Analysis of Plastidial Octanoyltransferase Mutants of Arabidopsis thaliana

Metabolites, 2019
Plant de novo fatty acid synthesis takes place in the plastid using acetyl-coenzyme A (acetyl-CoA) as the main precursor. This first intermediate is produced from pyruvate through the action of the plastidial pyruvate dehydrogenase complex (PDH), which ...
Raquel Martins-Noguerol, Antonio Javier Moreno-Pérez, Sebastien Acket, Salim Makni, Rafael Garcés, Adrián Troncoso-Ponce, Joaquín J. Salas, Brigitte Thomasset, Enrique Martínez-Force   +8 more
doaj   +1 more source

Multiple molecular mechanisms cause reproductive isolation between three yeast species. [PDF]

PLoS Biology, 2010
Nuclear-mitochondrial conflict (cytonuclear incompatibility) is a specific form of Dobzhansky-Muller incompatibility previously shown to cause reproductive isolation in two yeast species.
Jui-Yu Chou, Yin-Shan Hung, Kuan-Huei Lin, Hsin-Yi Lee, Jun-Yi Leu   +4 more
doaj   +1 more source

Characterization of Helianthus annuus Lipoic Acid Biosynthesis: The Mitochondrial Octanoyltransferase and Lipoyl Synthase Enzyme System

Frontiers in Plant Science, 2021
Lipoic acid (LA, 6,8-dithiooctanoic acid) is a sulfur containing coenzyme essential for the activity of several key enzymes involved in oxidative and single carbon metabolism in most bacteria and eukaryotes. LA is synthetized by the concerted activity of
Raquel Martins-Noguerol, Sébastien Acket, M. Adrián Troncoso-Ponce, Rafael Garcés, Brigitte Thomasset, Mónica Venegas-Calerón, Joaquín J. Salas, Enrique Martínez-Force, Antonio J. Moreno-Pérez   +8 more
doaj   +1 more source

Pseudomonas aeruginosa and Staphylococcus aureus Display Differential Proteomic Responses to the Silver(I) Compound, SBC3

Antibiotics, 2023
The urgent need to combat antibiotic resistance and develop novel antimicrobial therapies has triggered studies on novel metal-based formulations. N-heterocyclic carbene (NHC) complexes coordinate transition metals to generate a broad range of anticancer
Magdalena Piatek, Cillian O’Beirne, Zoe Beato, Matthias Tacke, Kevin Kavanagh   +4 more
doaj   +1 more source

Identification and Solution Structures of a Single Domain Biotin/Lipoyl Attachment Protein from Bacillus subtilis [PDF]

Journal of Biological Chemistry, 2006
Protein biotinylation and lipoylation are post-translational modifications, in which biotin or lipoic acid is covalently attached to specific proteins containing biotin/lipoyl attachment domains. All the currently reported natural proteins containing biotin/lipoyl attachment domains are multidomain proteins and can only be modified by either biotin or ...
Gaofeng, Cui, Beiyan, Nan, Jicheng, Hu, Yiping, Wang, Changwen, Jin, Bin, Xia   +5 more
openaire   +2 more sources

Cuproptosis-related gene FDX1 expression correlates with the prognosis and tumor immune microenvironment in clear cell renal cell carcinoma

Frontiers in Immunology, 2022
BackgroundCuproptosis, a newly discovered form of cell death, is regulated by protein lipoylation and is related to mitochondrial metabolism. However, further research is needed to determine how the cuproptosis-related gene ferredoxin 1 (FDX1) affects ...
Tao Wang, Yufeng Liu, Qing Li, Yang Luo, Dawei Liu, Bin Li   +5 more
doaj   +1 more source